Title Site-specific protein labeling [electronic resource] : methods and protocols / edited by Arnaud Gautier, Marlon J. Hinner
Imprint New York, NY : Humana Press ; Springer, c2015
book jacket
Descript 1 online resource (xii, 267 p.) : ill. (some col.)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
Series Methods in Molecular Biology, 1064-3745 ; 1266
Methods in molecular biology ; 1266
Note Includes bibliographical references and index
How FlAsH got its dparkle : historical recollections of the biarsenical-tetracysteine tag / B. Albert Griffin, Stephen R. Adams, and Roger Y. Tsien - - Site-specific protein labeling in the pharmaceutical industry : experiences from novartis drug discovery / Lukas Leder -- Getting across the cell membrane : an overview for small molecules, peptides, and proteins / Nicole J. Yang and Marlon J. Hinner -- Considerations and protocols for the synthesis of custom protein labeling probes / Ivan R. Corrêa Jr. -- 2-Cyanobenzothiazole (CBT) condensation for site- specific labeling of proteins at the terminal cysteine residues / Lina Cui and Jianghong Rao -- Fluorescent labeling for patch-clamp fluorometry (PCF) measurements of real-time protein motion in ion channels / Thomas K. Berger and Ehud Y. Isacoff -- Fluorescent labeling of SNAP-tagged proteins in cells / Gražvydas Lukinavičius, Luc Reymond, and Kai Johnsson -- Halotag technology for specific and covalent labeling of fusion proteins / Hélène A. Benink and Marjeta Urh -- Ligation of synthetic peptides to proteins using semisynthetic protein trans-splicing / Julian C.J. Matern ... [et al.] -- Chemical-tag labeling of proteins using fully recombinant split inteins / Anne-Lena Bachmann ... [et al.] -- Phage selection assisted by Sfp phosphopantetheinyl transferase-catalyzed site-specific protein labeling / Bo Zhao ... [et al.] -- Site-specific biotinylation of purified proteins using BirA / Michael Fairhead and Mark Howarth -- Site-specific labeling of proteins via sortase : protocols for the molecular biologist / Maximilian Wei-Lin Popp -- BONCAT: metabolic labeling, click chemistry, and affinity purification of newly synthesized proteomes / Peter Landgraf, Elmer R. Antileo, Erin M. Schuman, and Daniela C. Dieterich -- Genetic encoding of unnatural amino acids for labeling proteins / Kathrin Lang , Lloyd Davis, and Jason W. Chin -- Labeling proteins by affinity-guided DMAP chemistry / Tomonori Tamura and Itaru Hamachi -- Ligand-directed tosyl chemistry for selective native protein labeling in vitro, in cells, and in vivo / Shinya Tsukiji and Itaru Hamachi
Online resource; title from PDF title page (SpringerLink, viewed January 23, 2015)
Link Print version: Site-specific protein labeling : methods and protocols 9781493922710
Subject Proteins -- Analysis -- Laboratory manuals
Electronic books
Alt Author Gautier, Arnaud
Hinner, Marlon J