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Title Clinical applications of PCR [electronic resource] / edited by Rajyalakshmi Luthra, Rajesh R. Singh, and Keyur P. Patel
Imprint New York : Humana Press ; Springer, c2016
book jacket
Edition 3rd ed
Descript 1 online resource (x, 191 p.) : illustrations
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
Series Methods in molecular biology, 1064-3745 ; 1392
Methods in molecular biology ; 1392
Note Includes bibliographical references and index
Targeted Q-PCR-based method for point mutation testing by analyzing circulating DNA for cancer management care / Alain R. Thierry -- COLD-PCR : applications and advantages / Zhuang Zuo and Kausar J. Jabbar -- PCR-based detection of DNA copy number variation / Meenakshi Mehrotra -- Emulsion PCR : techniques and applications / Rashmi Kanagal-Shamanna -- Digital PCR : principles and applications / Rashmi Kanagal-Shamanna -- Quantitative PCR for plasma epstein-barr virus loads in cancer diagnostics / Sanam Loghavi -- High-resolution melt curve analysis in cancer mutation screen / Meenakshi Mehrotra and Keyur P. Patel -- Locked nucleic acid probes (LNA) for enhanced detection of low-level, clinically significant mutations / Khedoudja Nafa, Meera Hameed, and Marie E. Arcila -- Genotyping of frequent mutations in solid tumors by PCR- based single-base extension and MassARRAY analysis / Alberto Paniz Mondolfi and Rajesh R. Singh -- Microfluidics-based PCR for fusion transcript detection / Hui Chen -- Polymerase chain reaction diagnosis of Leishmaniasis : a species-specific approach / Eglys González-Marcano ... [et al.] -- Detection of Trypanosoma cruzi by Polymerase Chain Reaction / María Elizabeth Márquez ... [et al.] -- PCR techniques in next-generation sequencing / Rashmi S. Goswami -- Single-cell quantitative PCR : advances and potential in cancer diagnostics / Chi Young Ok, Rajesh R. Singh, and Alaa A. Salim -- Quantitative real-time PCR : recent advances / Charanjeet Singh and Sinchita Roy-Chowdhuri -- PCR techniques in characterizing DNA methylation / Khalida Wani and Kenneth D. Aldape
This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques. The application of PCR has shown incredible value in the study of genomics and transcriptomics, not only for discovery but also for routineclinical applications, and it forms the cornerstone of personalized medicine. Written for the highly successfulMethods in Molecular Biologyseries, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative,Clinical Applications of PCR, Third Editionserves as an ideal guide for researchers aiming to understand the principles behind each application and for their implementation in the laboratory
Link Print version: Clinical applications of PCR. 3rd ed. 9781493933587
Subject Polymerase chain reaction -- Diagnostic use
Polymerase Chain Reaction -- methods
Electronic books
Alt Author Luthra, Rajyalakshmi
Singh, Rajesh R
Patel, Keyur P
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