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001 EBC259254
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006 m o d |
007 cr cnu||||||||
008 200713s2005 xx o ||||0 eng d
020 9781860946783|q(electronic bk.)
020 |z9781860944284
035 (MiAaPQ)EBC259254
035 (Au-PeEL)EBL259254
035 (CaPaEBR)ebr10126023
035 (CaONFJC)MIL186667
035 (OCoLC)935232548
040 MiAaPQ|beng|erda|epn|cMiAaPQ|dMiAaPQ
050 4 QP552.M44D54 2005
082 0 572
100 1 Dietrich, Jens
245 10 Strategies For Two-dimensional Crystallization Of Proteins
Using Lipid Monolayers
264 1 Singapore :|bWorld Scientific Publishing Company,|c2005
264 4 |c©2005
300 1 online resource (154 pages)
336 text|btxt|2rdacontent
337 computer|bc|2rdamedia
338 online resource|bcr|2rdacarrier
505 0 Intro -- Introduction -- Contents -- 1 Two-dimensional
Crystallization on Lipid Monolayers -- 1.1. Overview --
1.2. Non-specific Adsorption through Electrostatic
Interactions -- 1.3 Specific Adsorption via Ligands --
1.3.1. Molecular recognition between protein and lipid
headgroup -- Cholera toxin B/monosialoganglioside --
1.3.2. Functionalized lipids -- 1.3.2.1. Moiety-carrying
lipids -- Biotin-lipid streptavidin system -- Lipid-hapten
-- ATP-lipid -- Novobiocin-lipid -- Dichlorophenyl-lipids
-- Other specific lipid ligands -- 1.3.2.2. Metal-
chelating lipids (Histidine-tag/Ni2+ lipid headgroup) --
NTA-Nickel lipid headgroup design -- Histidine-tag --
Binding properties of Ni2+-Histidine -- Effect of
imidazole -- Effect of EDTA, DTT and various ions --
Effect of pH -- Effect of high salt, detergent, glycerol
and other buffer conditions -- Crystallization of His-tag
proteins -- 1.3.2.3. Multifunctionalized lipids --
1.3.2.4. Immobilization of proteins on biofunctionalized
surfaces using synthetic lipids -- Moiety-carrying lipids
-- Metal-chelating lipid -- 2 Two-dimensional
Crystallization of Membrane Proteins -- 2.1 Overview --
2.2. Naturally Occurring and Induced 2D Crystals -- 2.3.
2D Crystallization by Reconstitution into a Lipid Bilayer
-- 2.4 Surface Crystallization of Membrane Proteins -- 3
Methods to Monitor Surface Crystallization -- Introduction
to the Biophysical Studies of Interfaces -- 3.1. Fluidity
of the Monolayers -- 3.1.1. Measurement of the fluidity of
lipids -- 3.1.2. Fluidity of the lipid monolayer and
crystallization -- 3.1.3. How to modify the fluidity of a
lipid monolayer? -- Modification of the surface tension
imposed by the lipid at the interface -- Effect of
temperature -- Variation of the molecular structure of
lipids -- 3.2. Ellipsometry -- 3.3. Rigidity Measurements
-- 3.4. Brewster Angle Microscopy
505 8 3.5. X-Ray Reflectivity and X-Ray Grazing Incidence
Diffraction -- 3.5.1. X-Ray reflectivity -- 3.5.2. X-Ray
grazing incidence diffraction on monolayers at the surface
of water -- 3.6. Atomic Force Microscopy -- 4 Electron
Microscopic Observations and Image Analysis -- 4.1.
Transfer of the Monolayer onto an Electron Microscope Grid
-- Various approaches have been tried to overcome this
problem of transfer -- 4.2. Preparation of the Specimen
for Electron Microscopic Observation -- 4.2.1. Negative
staining -- 4.2.2. Cryo-electron microscopy -- 4.3. The
Electron Microscope -- 4.3.1. Formation of the image --
4.3.2. Instrumental advances -- 4.4. Image Processing --
4.4.1. Averaging imaging to improve the resolution --
4.4.2. Three-dimensional information -- 4.4.3. Combining
modelling and interpretation of results -- 5 Practical
Considerations -- 5.1. General Practical Considerations
for Soluble Proteins or Multi-Protein Complexes -- 5.2.
Membrane Protein Crystallization -- 5.3. Single Particle
Observation -- 6 Other New Methods -- 6.1. DNA Scaffolds -
- 6.2. Nanotubes -- Appendix -- Glossary -- Bibliography -
- Index
520 Strategies for Two-Dimensional Crystallization of Proteins
UsingLipid Monolayers presents an overview of different
methods thatlead to structure determination by electron
microscopy. These methodshave proven to be extremely
successful, especially for elucidating thestructure of
membrane proteins
588 Description based on publisher supplied metadata and other
sources
590 Electronic reproduction. Ann Arbor, Michigan : ProQuest
Ebook Central, 2020. Available via World Wide Web. Access
may be limited to ProQuest Ebook Central affiliated
libraries
650 0 Membrane proteins.;Crystallization.;Electron microscopy --
Technique
655 4 Electronic books
700 1 Venien-bryan, Catherine
776 08 |iPrint version:|aDietrich, Jens|tStrategies For Two-
dimensional Crystallization Of Proteins Using Lipid
Monolayers|dSingapore : World Scientific Publishing
Company,c2005|z9781860944284
856 40 |uhttps://ebookcentral.proquest.com/lib/sinciatw/
detail.action?docID=259254|zClick to View
