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Title Bacterial pathogenesis. Part A, Identification and regulation of virulence factors [electronic resource] / edited by Virginia L. Clark, Patrik M. Bavoil
Imprint San Diego : Academic Press, c1994
book jacket
Descript 1 online resource (xxx, 789 p.) : ill
Series Methods in enzymology, 0076-6879 ; v. 235
Methods in enzymology ; v. 235
Note Includes bibliographical references and indexes
Section I. Evaluations of virulence in animal models -- section II. Species and strain identification -- section III. Bacterial cell fractionation -- section IV. Microbial acquisition of iron -- section V. Genetics and regulation -- section VI. Enzyme and toxin assays
Description based on print version record
This volume of Methods in Enzymology presents methods for the isolation and identification of bacterial pathogens and associated virulence determinants. It will enable the researcher to determine if closed genes or isolated gene products are involved in virulence. Major Topics Covered Include * Animal model system to determine bacterial virulence * Epidemiological techniques for the identification of bacterial strains and species * Protocols for the purification of subcellular bacterial products frequently associated with virulence * Determination of the means by which bacteria acquire iron * Methods to generate mutants, to construct isogenic strains by allelic exchange, to identify bacterial genes only expressed during infection of the host, and to study regulation of selected bacterial virulence factors * Methods for the assay of destructive bacterial enzymes and toxins
Link Print version: Bacterial pathogenesis. Part A, Identification and regulation of virulence factors. San Diego : Academic Press, c1994 0121821366 (OCoLC)30378433
Subject Pathogenic bacteria
Virulence (Microbiology)
Bacterial diseases -- Animal models
Electronic books
Alt Author Clark, Virginia L., 1945-
Bavoil, Patrik M
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