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Author Butler, John M
Title Advanced Topics in Forensic DNA Typing
Imprint San Diego : Elsevier Science & Technology, 2011
book jacket
Edition 3rd ed
Descript 1 online resource (699 pages)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
Note Front Cover -- Advanced Topics in Forensic DNA Typing: Methodology -- Copyright Page -- Contents -- Foreword -- Introduction -- Acknowledgments -- About the Author -- 1. Sample Collection, Storage, and Characterization -- Steps in DNA Testing Process -- Sample Collection -- Sample Storage and Transport of DNA Evidence -- Sample Characterization -- Contamination Concerns -- Reading List and Internet Resources -- 2. DNA Extraction Methods -- Purpose of DNA Extraction -- Early Techniques used for DNA Extraction -- Solid-Phase DNA Extraction Methods -- Differential Extraction -- Direct PCR to Bypass DNA Extraction -- Reading List and Internet Resources -- 3. DNA Quantitation -- Purpose of DNA Quantitation -- DNA Quantitation Methods -- Real-Time Quantitative PCR (QPCR) -- DNA Quantitation as a Gatekeeper -- Reading List and Internet Resources -- 4. PCR Amplification: Capabilities and Cautions -- PCR Background and Basics -- PCR Components & Controls -- Multiplex PCR -- PCR Inhibition -- Modifications to Improve PCR -- Stochastic Effects -- Precautions Against Contamination -- New Capabilities with Improvements in PCR -- Reading List and Internet Resources -- 5. Short Tandem Repeat (STR) Loci and Kits -- Genetic Markers and Repeated DNA Sequences -- Core and Common STR Markers -- Commercial STR Kits -- Information on 23 Core and Commonly Used STR Loci -- STR Allele Nomenclature -- Null (Silent) Alleles -- STR Typing -- Gender Identification with Amelogenin -- STRBase: an Internet Resource -- Reading List and Internet Resources -- 6. Capillary Electrophoresis: Principles and Instrumentation -- Requirements for STR Typing -- Principles and Components of Capillary Electrophoresis -- Electrokinetic Injection -- DNA Separation -- Fluorescence Detection -- Instrumentation -- Reading List and Internet Resources -- 7. Quality Assurance and Validation
Importance of Quality Assurance -- Levels of QA/QC -- Levels of Validation -- Other Aids to Quality Assurance -- Reading List and Internet Resources -- 8. DNA Databases: Uses and Issues -- Value of DNA Databases -- National DNA Databases around the World -- The U.S. National DNA Database -- DNA Database Laws in the United States -- Issues and Concerns with DNA Databases -- When There are No Hits after a Database Search -- Concerns of DNA Database Critics -- Reading List and Internet Resources -- 9. Missing Persons and Disaster Victim Identification Efforts -- Missing Persons Investigations -- Disaster Victim Identification (DVI) -- Issues Faced During Disaster Victim Identification Efforts -- Reading List and Internet Resources -- 10. Degraded DNA -- Degraded DNA -- Attempts with DNA Repair following DNA Damage -- Reading List and Internet Resources -- 11. Low-Level DNA Testing: Issues, Concerns, and Solutions -- Introduction to Issues and Historical Overview -- Assorted Topics -- Reading List and Internet Resources -- 12. Single Nucleotide Polymorphisms and Applications -- Single Nucleotide Polymorphisms (SNPs) -- SNP Analysis Techniques -- Applications -- Ethical Considerations -- Insertion-Deletion Polymorphisms -- Summary and Final Thoughts -- Reading List and Internet Resources -- 13. Y-Chromosome DNA Testing -- Lineage Markers -- Applications of ChrY Testing -- ChrY Structure -- Y-STR Markers -- Y-STR Haplotype Databases -- Interpretation of Y-STR Results -- Y-STR Use in Forensic Casework -- Y-SNPs and Haplogroups -- ChrY Historical and Genealogical Studies -- Summary -- Reading List and Internet Resources -- 14. Mitochondrial DNA Analysis -- Characteristics of mtDNA -- Mitochondrial DNA Sequencing in Forensic Casework -- Interpretation of mtDNA Results -- Issues Impacting Interpretation -- Laboratories Performing mtDNA Testing
Screening Assays for mtDNA Typing -- Population Databases -- Reading List and Internet Resources -- 15. X-Chromosome Analysis -- ChrX Inheritance and Application -- Applications of ChrX Testing -- X-STR Markers Commonly Used -- X-STR locus HumARA no longer used -- X-STR Kits and Multiplex Assays -- Population Data Collection and Other Studies -- Reading List and Internet Resources -- 16. Non-human DNA -- Domestic Animal DNA Testing -- Species Identification -- Wildlife DNA Testing -- Plant & Pollen DNA Testing -- Identification through Human Parasites -- Microbial Forensics -- Challenges with Presenting Non-Human DNA in Court -- Reading List and Internet Resources -- 17. New Technologies and Automation -- Rapid DNA Analysis -- Mass Spectrometry -- Pyrosequencing -- Next-Generation DNA Sequencing -- Laboratory Automation -- Summary and Final Thoughts -- Reading List and Internet Resources -- 18. Legal Aspects of DNA Testing and the Scientific Expert in Court -- Including input from interviews with Dr. Robin Cotton, Ted Hunt, Brad Bannon, the Orange County (CA) District Attorney's DNA Unit, and Dr. Charlotte Word -- Science and Law: Different Perspectives -- Legal Terms, Concepts, and Important Court Cases -- The Courtroom Scene -- The Scientific Expert Witness -- Admissibility of Evidence -- Types of DNA Cases and Opposing Counsel Tactics -- Jury Comprehension of DNA Evidence -- Facing the "CSI Effect" -- Final Thoughts -- Reading List and Internet Resources -- Appendix 1 Reported Sizes and Sequences of STR Alleles -- CSF1PO -- FGA -- TH01 -- TPOX -- VWA -- D3S1358 -- D5S818 -- D7S820 -- D8S1179 (listed as D6S502 in early papers) -- D13S317 -- D16S539 -- D18S51 -- D21S11 -- D2S1338 -- D19S433 -- Penta D -- Penta E -- D1S1656 -- D2S441 -- D10S1248 -- D12S391 -- D22S1045 -- SE33
Appendix 2 Familial DNA Searches: Potential, Pitfalls, and Privacy Concerns -- Pitfalls of False Positives and False Negatives -- Low Success Rates -- Lineage Markers to Improve Success Rates -- Supposed "Genetic Surveillance" of Families -- Appendix 3 List of Suppliers for DNA Instruments, Reagents, Services -- Appendix 4 Interviews Supporting Legal Aspects of DNA Testing -- Interview with Dr. Robin Cotton on Being an Expert Witness -- Interview with Dr. Charlotte Word on Admissibility Hearings -- Interview with Ted Hunt, J.D., on a Prosecutor's Perspective -- Interview with the Orange County (California) District Attorney's DNA Unit -- Interview with Bradley Bannon, J.D., on a Defense Attorney's Perspective -- Subject Index
Intended as a companion to the Fundamentals of Forensic DNA Typing volume published in 2009, Advanced Topics in Forensic DNA Typing: Methodology contains 18 chapters with 4 appendices providing up-to-date coverage of essential topics in this important field and citation to more than 2800 articles and internet resources. The book builds upon the previous two editions of John Butler's internationally acclaimed Forensic DNA Typing textbook with forensic DNA analysts as its primary audience.  This book provides the most detailed information written to-date on DNA databases, low-level DNA, validation, and numerous other topics including a new chapter on legal aspects of DNA testing to prepare scientists for expert witness testimony. Over half of the content is new compared to previous editions. A forthcoming companion volume will cover interpretation issues. Contains the latest information - hot-topics and new technologies Well edited, attractively laid out, and makes productive use of its four-color format Author John Butler is ranked as the number one "high-impact author in legal medicine and forensic science, 2001 to 2011" by
Description based on publisher supplied metadata and other sources
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2020. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
Link Print version: Butler, John M. Advanced Topics in Forensic DNA Typing: Methodology San Diego : Elsevier Science & Technology,c2011 9780123745132
Subject DNA fingerprinting.;Forensic genetics
Electronic books
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