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書名 Myogenesis [electronic resource] : methods and protocols / edited by Joseph X. DiMario
出版項 New York : Humana Press, c2012
國際標準書號 9781617793431 (electronic bk.)
1617793434 (electronic bk.)
book jacket
說明 1 online resource (xv, 558 p.) : ill
系列 Methods in molecular biology, 1064-3745 ; 798
Methods in molecular biology ; 798
附註 Includes index
Isolation and characterization of human fetal myoblasts -- Skeletal muscle satellite cells: Background and methods for isolation and analysis in a primary culture system -- Isolation of muscle stem cells by fluorescence activated cell sorting cytometry -- Mouse and human mesoangioblasts: Isolation and characterization from adult skeletal muscles -- Direct electrical stimulation of myogenic cultures for analysis of muscle fiber type control -- Single muscle-fiber isolation and culture for cellular, molecular, pharmacological, and evolutionary studies -- Somite unit chronometry to analyze teratogen phase specificity in the paraxial mesoderm -- Analysis of skeletal muscle development in drosophila -- Immunocytochemistry to study myogenesis in zebrafish -- Immunofluorescent localization of proteins in caenorhabditis elegans muscle -- Resistance loading and signaling assays for oxidative stress in rodent skeletal muscle -- Analysis of skeletal muscle hypertrophy in models of increased loading -- Protein overexpression in skeletal muscle using plasmid-based gene transfer to elucidate mechanisms controlling fiber size -- In vivo measurement of muscle protein synthesis rate using the flooding dose technique -- Recombinant adeno-associated viral vector production and purification -- Generation of lentiviral vectors for use in skeletal muscle research -- Generating tamoxifen-inducible cre alleles to investigate myogenesis in mice -- Gene profiling studies in skeletal muscle by quantitative real-time polymerase chain reaction assay -- Analysis of lipid profiles in skeletal muscles -- Proteomic analysis of dystrophic muscle -- Detection of calcium release via ryanodine receptors -- Measurement of calcium release due to inositol trisphosphate receptors in skeletal muscle -- Detection of calcium sparks in intact and permeabilized skeletal muscle fibers -- Analysis of calcium transients in cardiac myocytes and assessment of the sarcoplasmic reticulum ca(2+)-ATPase contribution -- Analysis of muscle gene transcription in cultured skeletal muscle cells -- Analysis of fiber-type differences in reporter gene expression of [beta]-gal transgenic muscle -- Determination of gene promoter activity in skeletal muscles in vivo -- Determination of miRNA targets in skeletal muscle cells -- ShRNA-mediated gene knockdown in skeletal muscle -- Detection of NF-[kappa]B activity in skeletal muscle cells by electrophoretic mobility shift analysis -- Isolation of nuclei from skeletal muscle satellite cells and myofibers for use in chromatin immunoprecipitation assays -- An improved restriction enzyme accessibility assay for analyzing changes in chromatin structure in samples of limited cell number -- ChIP-enriched in silico targets (ChEST), a ChIP-on-ChIP approach applied to analyzing skeletal muscle genes
Description based on print version record
鏈接 Print version: Myogenesis. New York Humana Press ; Springer, c2012 9781617793424 (OCoLC)733237681
主題 Muscle Development
Myogenesis -- Laboratory manuals
Life sciences
Cell Biology
Electronic books
Alt Author DiMario, Joseph X
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