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作者 Weckwerth, Wolfram
書名 The Handbook of Plant Metabolomics
出版項 Weinheim : John Wiley & Sons, Incorporated, 2013
©2013
國際標準書號 9783527669899 (electronic bk.)
9783527327775
book jacket
版本 1st ed
說明 1 online resource (452 pages)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
系列 Molecular Plant Biology Ser
Molecular Plant Biology Ser
附註 The Handbook of Plant Metabolomics -- Contents -- Preface -- List of Contributors -- Part I Central Metabolism -- 1 Metabolic Profiling of Plants by GC-MS -- 1.1 Introduction -- 1.2 Methods and Protocols -- 1.2.1 Sample Preparation -- 1.2.1.1 Sampling -- 1.2.1.2 Homogenization and Extraction -- 1.2.1.3 Procedure for Polar Extraction of Metabolites -- 1.2.2 Chemical Derivatization: Methoxymation and Silylation -- 1.2.2.1 Procedure for the Chemical Derivatization of Plant Extracts -- 1.2.3 GC-MS Analysis -- 1.2.3.1 Procedure to Acquire GC-MS Data -- 1.2.4 Data Preprocessing and Export -- 1.2.4.1 Procedure for Postacquisition Data Preprocessing -- 1.2.4.2 Data Analysis and Statistics -- 1.2.4.3 Procedure for Postacquisition Data Analysis -- 1.3 Applications of the Technology -- 1.4 Perspectives -- References -- 2 Isotopologue Profiling - Toward a Better Understanding of Metabolic Pathways -- 2.1 Introduction -- 2.2 Methods and Protocols to Determine Isotopologues -- 2.2.1 Mass Spectrometry -- 2.2.2 Protocols for Isotopologue Profiling by GC-MS -- 2.2.2.1 Protein-Bound Amino Acids -- 2.2.2.2 Metabolic Intermediates and Polar Products -- 2.2.2.3 Carbohydrates -- 2.2.3 NMR Spectroscopy -- 2.2.4 Protocols for Isotopologue Profiling by NMR -- 2.2.5 Deconvolution of Isotopologue Data -- 2.2.6 Expanding the Metabolic Space by Retrobiosynthetic Analysis -- 2.3 Applications -- 2.3.1 Experiments Using [U-13C6] Glucose -- 2.3.2 Experiments Using 13CO2 -- 2.4 Perspectives -- References -- 3 Nuclear Magnetic Resonance Spectroscopy for Plant Metabolite Profiling -- 3.1 Introduction -- 3.2 Methods and Protocols -- 3.2.1 Sample Preparation -- 3.2.1.1 Harvesting Plant Material -- 3.2.1.2 Drying -- 3.2.1.3 Extraction -- 3.2.2 Data Acquisition -- 3.2.3 Standard 1H-NMR Spectroscopy -- 3.2.4 J-Resolved Spectroscopy -- 3.2.5 Data Analysis -- 3.3 Applications
3.3.1 1D 1H-NMR Spectroscopy -- 3.3.2 2D NMR Spectroscopy -- 3.3.2.1 J-Resolved Spectroscopy -- 3.3.2.2 COSYand TOCSY -- 3.3.2.3 HMBC and HMQC/HSQC -- 3.3.2.4 NOESYor ROESY (CAMELSPIN) -- 3.3.2.5 DOSY -- 3.3.3 Magic Angle Spinning -- 3.4 Perspectives -- References -- 4 Comprehensive Two-Dimensional Gas Chromatography for Metabolomics -- 4.1 Introduction -- 4.2 Methods and Protocols -- 4.2.1 Instrumentation -- 4.2.2 Sample Preparation and Analysis -- 4.2.3 Data Processing -- 4.2.4 Metabolic Fingerprinting -- 4.2.5 Quantitative Analysis of Selected Metabolites -- 4.3 Applications of the Technology -- 4.3.1 Data Analysis -- 4.3.2 Literature -- 4.4 Perspectives -- References -- 5 MALDI Mass Spectrometric Imaging of Plants -- 5.1 Introduction -- 5.1.1 Sample Preparation -- 5.1.2 Data Acquisition -- 5.1.3 Data Processing -- 5.2 Methods and Protocols -- 5.2.1 Sample Preparation and Handling -- 5.2.1.1 Intact Tissues -- 5.2.1.2 Cryosectioning -- 5.2.2 Matrix Deposition -- 5.2.2.1 Paintbrush (Figure 5.2) -- 5.2.2.2 Sublimation (Figure 5.3) -- 5.2.3 MALDI-MS Imaging Measurement -- 5.2.3.1 Bruker Ultraflex Instruments -- 5.2.3.2 Waters MALDI Micro MX -- 5.3 Imaging Intact Tissues and Objects -- 5.4 Future Perspectives -- References -- 6 Medicago truncatula Root and Shoot Metabolomics: Protocol for the Investigation of the Primary Carbon and Nitrogen Metabolism Based on GC-MS -- 6.1 Introduction -- 6.2 Methods and Protocols -- 6.2.1 Equipment and Software -- 6.2.2 Buffers and Chemicals -- 6.2.3 Plant Material and Harvest -- 6.2.4 Extraction -- 6.2.5 Derivatization -- 6.2.6 GC-MS Setup for the Analysis -- 6.2.7 Metabolite Identification and Quantification: Data Matrix Processing -- 6.2.8 Data Mining -- 6.3 Applications of the Technology -- 6.4 Perspectives -- References -- Part II Secondary and Lipid Metabolism
7 Study of the Volatile Metabolome in Plant-Insect Interactions -- 7.1 Introduction -- 7.1.1 Plant-Insect Interactions -- 7.1.2 Significance of Volatile Plant Metabolites -- 7.1.3 Study of the Plant Volatile Metabolome in Plant-Insect Interactions -- 7.1.3.1 Setting Up of Biological Experiments -- 7.1.3.2 Sampling, Quenching, and Sample Preparation -- 7.1.3.3 Headspace Extraction and Measurement by GC-MS -- 7.1.3.4 Data Handling -- 7.1.3.5 Biological Interpretation -- 7.2 Methods and Protocols -- 7.2.1 Permanent Breed of Insects -- 7.2.2 Cultivation of Grapevine Plants and Inoculation with Phylloxera -- 7.2.2.1 Materials -- 7.2.2.2 Procedures -- 7.2.3 Sampling and Quenching of Plant Tissue (Roots and Leaves) -- 7.2.3.1 Sampling and Quenching of Root Tips -- 7.2.3.2 Sampling and Quenching of Grapevine Leaves -- 7.2.4 Milling and Weighing of Plant Tissue (Roots and Leaves) -- 7.2.4.1 Milling and Weighing of Root Samples -- 7.2.4.2 Milling and Weighing of Leaf Samples -- 7.2.5 Measurement - Automated HS-SPME Extraction and GC-MS Analysis -- 7.2.5.1 Materials -- 7.2.5.2 SPME Method -- 7.2.5.3 GC Method -- 7.2.5.4 MS Settings -- 7.2.6 Data Processing with AMDIS -- 7.2.6.1 An In-House Reference Library Has to be Established in Advance -- 7.2.6.2 Generation of RI Calibration File -- 7.2.6.3 Batch Job Analysis for the Simultaneous Processing of Multiple Sample Chromatograms -- 7.2.7 Statistics/Chemometrics -- 7.2.7.1 Univariate Statistics -- 7.2.7.2 Multivariate Statistics -- 7.3 Applications of the Technology -- 7.4 Perspectives -- References -- 8 Metabolomics in Herbal Medicine Research -- 8.1 Introduction -- 8.2 Methods and Protocols -- 8.2.1 Materials -- 8.2.1.1 Reagents -- 8.2.1.2 Equipment -- 8.2.2 Procedures -- 8.2.2.1 Sample Handling for Medicinal Plants -- 8.2.2.2 Sample Preparation for LC-MS Analysis -- 8.2.2.3 LC-MS Analysis
8.2.2.4 HPLC-Photodiode Array (PDA) MS Setup and Analysis -- 8.2.2.5 GC-MS Analysis -- 8.2.2.6 Plant Extract Preparation for GC-MS Analysis -- 8.2.2.7 GC-MS Parameters and Analysis -- 8.2.2.8 LC-MS and GC-MS Data Analysis -- 8.2.2.9 LC-SPE-NMR Analysis -- 8.2.2.10 Sample Preparation and LC-SPE-NMR Analysis -- 8.2.2.11 HPLC-SPE-NMR Data Analysis -- 8.3 Applications -- 8.4 Perspectives -- References -- 9 Integrative Analysis of Secondary Metabolism and Transcript Regulation in Arabidopsis thaliana -- 9.1 Introduction -- 9.2 Methods and Protocols -- 9.2.1 Metabolome Analysis of Plant Secondary Metabolites -- 9.2.1.1 Sample Preparation -- 9.2.1.2 Data Acquisition -- 9.2.1.3 Preparation of Metabolite Accumulation Data from the Raw Chromatogram Data -- 9.2.2 Preparation of Combined Data Matrix -- 9.2.2.1 Preparation of Gene Expression Data -- 9.2.2.2 Combination of Data Matrices -- 9.2.3 Data Mining -- 9.2.3.1 BL-SOM Analysis -- 9.2.3.2 Correlation Analysis -- 9.2.3.3 Principal Component Analysis and Application of Other Data Mining Techniques -- 9.3 Applications of the Technology -- 9.4 Perspectives -- References -- 10 Liquid Chromatographic-Mass Spectrometric Analysis of Flavonoids -- 10.1 Introduction -- 10.1.1 Role of Flavonoids and Their Derivatives in Biological Systems -- 10.1.2 Preparation of Biological Material for Metabolomic Analysis and/or Metabolite Profiling -- 10.1.3 Instrumental Considerations -- 10.2 Methods and Protocols: Liquid Chromatography-Mass Spectrometry of Flavonoids -- 10.2.1 General Remarks -- 10.2.2 Plant Cultivation Conditions -- 10.2.3 Preparation of Biological Material with Biotechnological Methods (Callus, Cell, or Hairy Root Cultures) -- 10.2.4 Extraction of Plant Tissue or Biotechnologically Prepared Material -- 10.2.4.1 Extraction Procedure -- 10.2.5 Solid-Phase Extraction of Culture Medium or Apoplastic Fluids
10.2.6 Preparation of Samples for LC-MS Analyses -- 10.2.7 Chromatographic Protocols for Separation of Flavonoid Glyconjugates -- 10.2.8 Control of Ionization Parameters During Mass Spectrometric Analysis and Identification of Compounds During LC-MS Metabolite Profiling -- 10.3 Applications of the Technology -- 10.4 Perspectives -- References -- 11 Introduction to Lipid (FAME) Analysis in Algae Using Gas Chromatography-Mass Spectrometry -- 11.1 Introduction -- 11.2 Methods and Experimental Protocol -- 11.2.1 Extraction -- 11.2.2 Bound and Free Fatty Acids -- 11.2.3 Pigments -- 11.2.4 Contaminants -- 11.2.5 Derivatization -- 11.2.6 GC-MS System -- 11.2.7 Identification -- 11.2.8 Protocols -- 11.2.8.1 Protocol I -- 11.2.8.2 Protocol II -- 11.2.9 GC-MS Instrument and Conditions -- 11.3 Application and Perspective -- References -- 12 Multi-Gene Transformation for Pathway Engineering of Secondary Metabolites -- 12.1 Introduction -- 12.2 Methods and Protocols -- 12.2.1 Chemicals -- 12.2.2 Plasmid Construction of Multi-Gene Transformation -- 12.2.3 Preparation of Dual Terminator (DT) Fragment by PCR-Based Overlap Extension Method -- 12.2.4 Plasmid Construction of pUHR KS CSPS Thsp -- 12.2.5 Construction of pHSG299 CSPS 35S-CYP88-DT (Figure 12.2a) -- 12.2.6 Construction of pHSG299 CSPS 35S-CYP72-DT2 (Figure 12.2a) -- 12.2.7 Construction of pHSG299-CYP93(RNAi)-DT (Figure 12.2a) -- 12.2.8 Construction of pUHR KS CSPS Thsp-CYP88-CYP72-CYP93 (RNAi) -- 12.2.9 Transformation of Soybean by Particle Bombardment -- 12.2.9.1 Preparation of Embryogenic Suspension Tissue Culture -- 12.2.9.2 Preparation of Plasmid DNA for Particle Bombardment -- 12.2.9.3 Conditions of Particle Bombardment -- 12.2.9.4 Selection and Generation of Transgenic Soybean Plants -- 12.2.10 GC-MS Analysis for Triterpene Glycone -- 12.2.10.1 Extraction of Metabolite
12.2.10.2 Acid Treatment of Extracted Metabolites
This is the newest title in the successful Molecular Plant Biology Handbook Series. Just like the other titles in the series this new book presents an excellent overview of different approaches and techniques in Metabolomics. Contributors are either from ivy-league research institutions or from companies developing new technologies in this dynamic and fast-growing field. With its approach to introduce current techniques in plant metabolomics to a wider audience and with many labs and companies considering to introduce metabolomics for their research, the title meets a growing market. The Kahl books are in addition a trusted brand for the plant science community and have always sold above expectations
Description based on publisher supplied metadata and other sources
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2020. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
鏈接 Print version: Weckwerth, Wolfram The Handbook of Plant Metabolomics Weinheim : John Wiley & Sons, Incorporated,c2013 9783527327775
主題 Plants -- Metabolism -- Handbooks, manuals, etc.;Plant metabolites -- Handbooks, manuals, etc
Electronic books
Alt Author Kahl, Guenter
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