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作者 Wang, Chengming
書名 Veterinary PCR Diagnostics
出版項 SAIF Zone : Bentham Science Publishers, 2012
國際標準書號 9781608053483 (electronic bk.)
book jacket
版本 1st ed
說明 1 online resource (144 pages)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
附註 01 Title.pdf -- 02 Cover Page -- 03 REVISED eBooks End User License Agreement-Website -- 04 Content -- 05 Foreword -- 06 Preface -- 07 list of Contributors -- 08 Chapter 1_Loftis -- 09 Chapter 2_Marijke -- 10 Chapter 3_Shaheen -- 11 Chapter 4_Lilenbaum -- 12 Chapter 5_Li -- 13 Chapter 6_Wu -- 14 Chapter 7_Gentilini -- 15 Index
PCR (Polymerase Chain Reaction) technology has become an indispensable component of routine veterinary diagnostics. However, a number of pitfalls and limiting factors affect its sensitivity and specificity of detection. It is imperative that veterinary PCR diagnosticians include such considerations in their work. Extensive experience with PCR technology in both research and diagnostic applications enables researchers to pinpoint these practical limitations, and therefore instruct the user in approaches that avoid these common errors.This E-book discusses the basic concepts, chemistries, and instrumentation of standard and real-time PCR, and includes present applications and future perspectives for veterinary diagnostics. Critical pitfalls have been highlighted in this text with regard to veterinary PCR diagnostics which include: i) choice of platform technologies for PCR diagnosis; ii) construction of optimized PCR primers and probes to ensure its highest specificity and sensitivity; iii) correct sampling and efficient methods to preserve and release nucleic acids; iv) maximization of PCR amplification efficiency; and v) avoidance and monitoring of contamination in Veterinary PCR Diagnostics. This eBook should be a valuable reference for all veterinary professionals interested in this modern diagnostic tool
Description based on publisher supplied metadata and other sources
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2020. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
鏈接 Print version: Wang, Chengming Veterinary PCR Diagnostics SAIF Zone : Bentham Science Publishers,c2012 9781608055722
主題 Polymerase chain reaction -- Diagnostic use.;Polymerase chain reaction.;DNA probes.;Microorganisms -- Identification
Electronic books
Alt Author Kaltenboeck, Bernhard
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