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書名 Sirtuins : methods and protocols / edited by Matthew D. Hirschey, Institute of Molecular Physiology, Duke University Medical Center, Durham, NC, USA
出版項 New York : Humana Press, 2013
國際標準書號 9781627036375 (electronic bk.)
1627036377 (electronic bk.)
國際標準號碼 10.1007/978-1-62703-637-5
book jacket
說明 1 online resource (xi, 314 pages) : illustrations (some color)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
系列 Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1077
Methods in molecular biology ; 1077
附註 Introduction : sirtuins in aging and diseases / Leonard Guarente -- Sirtuins in yeast : phenotypes and tools / Scott Tsuchiyama ... [et al.] -- C. elegans sirtuins / Mohan Viswanathan and Heidi A. Tissenbaum -- Genetic and biochemical tools for investigating sirtuin function in Drosophila melanogaster / Jason G. Wood, Rachel Whitaker, and Stephen L. Helfand -- Generating mammalian sirtuin tools for protein- interaction analysis / Kathleen A. Hershberger ... [et al.] -- Mass spectrometry-based detection of protein acetylation / Yu Li ... [et al.] -- SILAC-based quantification of Sirt1-responsive lysine acetylome / Yue Chen, Gozde Colak, and Yingming Zhao -- Targeted quantitation of acetylated lysine peptides by selected reaction monitoring mass spectrometry / Matthew J. Rardin, Jason M. Held, and Bradford W. Gibson -- Identification of deacetylase substrates with the biotin switch approach / J. Will Thompson, Alex Robeson, and Joshua L. Andersen -- Assaying chromatin sirtuins / Lei Zhong ... [et al.] -- Measurement of sirtuin enzyme activity using a substrate-agnostic fluorometric nicotinamide assay / Basil P. Hubbard and David A. Sinclair -- Detecting sirtuin-catalyzed deacylation reactions using 32P-labeled NAD and thin-layer chromatography / Anita Zhu, Xiaoyang Su, and Hening Lin -- Chemical acetylation and deacetylation / Kristofer S. Fritz -- Accurate measurement of nicotinamide adenine dinucleotide (NAD+) with high-performance liquid chromatography / Jun Yoshino and Shin-ichiro Imai -- In vivo measurement of the acetylation state of sirtuin substrates as a proxy for sirtuin activity / John Dominy, Pere Puigserver, and Carles Cantó -- Oxygen flux analysis to understand the biological function of sirtuins / Dongning Wang ... [et al.] -- Emerging links between sirtuins and autophagy / In Hye Lee, Jeanho Yun, and Toren Finkel -- Methods to study the role of sirtuins in genome stability / Paloma Martínez-Redondo and Alejandro Vaquero -- Circadian measurements of sirtuin biology / Kathryn Moynihan Ramsey ... [et al.] -- Utilizing calorie restriction to evaluate the role of sirtuins in healthspan and lifespan of mice / Jessica Curtis and Rafael de Cabo
Includes index
Featuring a diverse array of model organisms and scientific techniques, Sirtuins: Methods and Protocols collects detailed contributions from experts in the field addressing this vital family of genes. Opening with methods to generate sirtuin biology tools, the book continues by covering methods to identify sirtuin substrates, to measure sirtuin activity, and to study sirtuin biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Sirtuins: Methods and Protocols presents detailed protocols for sirtuin research that can be followed directly or modified to investigate new areas of sirtuin biology
Description based on online resource; title from PDF title page (SpringerLink, viewed September 9, 2013)
主題 Sirtuins -- Laboratory manuals
Life Sciences
Animal Genetics and Genomics
Electronic books
Alt Author Hirschey, Matthew D., editor of compilation
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