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Title Neurotrophic factors : methods and protocols / edited by Stephen D. Skaper
Imprint New York, NY : Humana Press, [2018]
book jacket
Edition Second edition
Descript 1 online resource (xv, 458 pages) : illustrations (some color)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
text file PDF rda
Series Methods in Molecular Biology, 1064-3745 ; 1727
Methods in molecular biology ; 1727
Note Includes bibliographical references and index
Online resource; title from PDF title page (SpringerLink, viewed December 14, 2017)
Neurotrophic factors : an overview / Stephen D. Skaper -- Ocular nerve growth factor (NGF) and NGF eye drop application as paradigms to investigate NGF neuroportective and reparative actions / Paola Tirassa, Pamela Rosso, and Angela Iannitelli -- Culture of neonatal rodent cortical, hoppocampal, and striatal neurons / Laura Facci and Stephen D. Skaper -- Culture of neonatal rodent microglia, astrocytes, and oligodendrocytes from the cortex, spinal cord, and cerebellum ; Central nervous system neuron-glia co-culture models and application to neuroprotective agents / Stephen D. Skaper and Laura Facci -- Preparation of adult rat sensory neuron cultures and their application to growth cone turning assays / Christine A. Webber and Douglas W. Zochodne -- Sensory neurons from tau transgenic mice and their utility in drug screening / Aviva M. Tolkovsky and Jack Brelstaff -- Culture of rat mesencephalic dopaminergic neurons and application to neurotoxic and neuroprotective agents / Stephen D. Skaper, Massimo Barbierato, Vanni Ferrari, Morena Zusso, and Laura Facci -- Culture of neurons and smooth muscle cells from the myenteric plexus of adult mice / Paola Brun and Hamid I. Akbarali -- Astrocyte/microglia cocultures as a model to study neuroinflammation / Cinzia Volont⥬ Adele De Ninno, and Susanna Amadio -- Oligodendrocyte progenitor cell cultures : a model to screen neurotrophic compounds for myelin repair / Massimo Barbierato, Stephen D. Skaper, and Laura Facci -- Cell enumeration assays : application of the MTT and sulforhodamine B assays to lipopolysacchardie-stimulated neonatal rodent microglia / Laura Facci and Stephen D. Skaper
Diolistic labeling and analysis of dendritic spines / M. Foster Olive, Armani P. Del Franco, and Cassandra D. Gipson -- Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses / Dimitar Dimitrov, Laurent Guillaud, Kohgaku Eguchi, and Tomoyuki Takahashi -- Methods for quantitative analysis of axonal cargo transport / Matias Alloatti, luciana Bruno, and Tomas L. Falzone -- Neuronal cell morphology in primary cerebellar granule cells using high-content analysis / Nicholas Radio and Steven Frank -- Nerve growth factor-induced angiogenesis : 1. endothelial cell tube formation assay ; 2. the quail chorioallantoic membrane assay / / Philip Lazarovici, Adi Lahiani, Galit Gincberg, Dikla Haham, Arnon Fluksman, Ofra Benny, Cezary Marcinkiewicz, and Peter I. Lelkes -- Rat pancreatic beta-cell culture / Myrian Velasco, Carlos Larqu⥬ Carlos Manlio D⩡z-Garc⩡, Cramen Sanchez-Solo, and Marcia Hiriart -- Growth and neurotrophic factors in embryonic stem cells / Micaela Pannella, Luciana Giardino, Laura Calzᡬ and Mercedes Fern⡮dez -- Site-specific direct labeling of neurotrophins and their receptors : from biochemistry to advanced imaging applications / Francesco Gobbo, Fulvio Bonsignore, Rosy Amodeo, Antonino Cattaneo, and laura Marchetti -- Isolation and culture of brain microvascular endothelial cells for In vitro blood-brain barrier studies / Hector Rosas-Hernandez, Elvis Cuevas, Susan M. Lantz, Merle G. Paule, and Sed F. Ali -- Preparation of rat hippocampal organotypic cultures and application to study amyloid-peptide toxicity / Maria rosanna Bronzuoli, Roberta Facchinetti, and Caterina Scuderi -- An animal model of alzheimer disease based on the intrahippocampal injection of amyloid -peptie (1-42) / Robert Facchinetti, Maria Rosanna Bronzuoli, and Caterina Scuderi -- Active induction of experimental autoimmune encephalomyelitis in C57BL/6 mice / Gabriella Contarini, Pietro Giusti, and Stephen D. Skaper -- A model of systemic inflammation to study neuroinflammation / Vincenzo Sorrenti, Pietro Giusti, and Morena Zusso
Spared nerve injury as a long-lasting model of neurpathic pain / Serena Boccella, Francesca Guida, Enza Palazzo, Ida Marabese, Vito de Novellis, Sabatino Maione, and Livio Luongo -- An In vivo compression model of spinal cord injury / Irene Paterniti, Emanuela Seposito, and Salvatore Cuzzocrea -- A controlled cortical impact preclinical model of traumatic brain injury / Michela Campolo, Emanuela Esposito, and Salvatore Cuzzorcrea -- Middle cerebral artery occlusion by an intraluminal suture method / Rosalia Crupi, Rosanna Di Paola, Emanuela Esposito, and Salvatore Cuzzocrea -- Primary motor neuron culture to promote cellular viability and myelination / Jun-Kyo Francis Suh and Sujin Hyung -- Culture of human primary bone cells and phenotype assessment / Paola Brun -- Recreating intestinal peristalsis in the petri dish / Rosa Espinosa-Luna, Alma R. Barajas-Espinosa, Fernando Ochoa-Cortez, and Carlos Barajas-L⯰ez -- Synthesis of biocompatible titanate nanofibers for effective delivery of neuroprotective agents / Asya Ozkizilcik, Roger Williams, Z. Ryan Tian, Dafin F. Muresanu, Aruna Sharma, and Hari S. Sharma -- Preparation and characterization of biocompatible chitosan nanoparticles for targeted brain delivery of peptides / Muge Yemisci, Secil Caban, Eduardo Fernandez-Megia, Yilmaz Capan, Patrick Couvreur, and Turgay Dalkara
This volume collects protocols and procedures utilizing cellular, tissue, and whole animal models that can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. It begins with chapters on the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia and glia-glia co-culture models, oligodendrocytes, and cell-based assays for the evaluation of cell vitality. This revised second edition expands to cover methodology encompassing site-specific direct labeling of neurotrophins and their receptors, angiogenesis assays, stem cells, pancreatic beta-cells, axonal transport, synapse biology, dendritic spine analysis, and brain endothelial cells. Subsequent chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides, as well as protocols describing nanofiber- and nanoparticle-based methods for brain delivery of neurotrophic agents. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.uthoritative and practical, Neurotrophic Factors: Methods and Protocols, Second Edition is a valuable resource for a wide audience of readers as they explore nervous system function and pathology
Link Print version: Neurotrophic factors. Second edition. New York, NY : Humana Press, [2018] 9781493975709 (DLC) 2017959194 (OCoLC)1005109934
Subject Neurotrophic functions -- Laboratory manuals
Neurons -- Physiology -- Laboratory manuals
Receptors, Growth Factor
Intercellular Signaling Peptides and Proteins
Nerve Tissue Proteins
Receptors, Peptide
Biological Factors
Receptors, Cell Surface
Amino Acids, Peptides, and Proteins
Chemicals and Drugs
Membrane Proteins
Nerve Growth Factors
Receptors, Nerve Growth Factor
Electronic books
Electronic books
Alt Author Skaper, Stephen D. (Stephen Drake), 1947- editor
Unif Title Neurotrophic factors (Skapper)
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