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Title Two-hybrid systems : methods and protocols / edited by Luis Oñate-Sánchez
Imprint New York, NY : Humana Press ; Springer, c2018
©2018
book jacket
Descript 1 online resource (xii, 355 pages) : illustrations (some color)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
Series Methods in molecular biology, 1064-3745 ; volume 1794
Methods in molecular biology ; 1794
Note Includes bibliographical references and index
Protein interactomics by two-hybrid methods / Soon Gang Choi, Aaron Richardson, Luke Lambourne, David E. Hill, and Marc Vidal -- Making the right choice : critical parameters of the Y2H systems / Jitender Mehla, J. Harry Caufield, and Peter Uetz -- Identification of protein-protein interactions using pool-array-based Yeast two-hybrid screening / Thomas Lathouwers, Jeroen Wagemans, and Rob Lavigne -- Detecting interactions of membrane proteins : the split-ubiquitin system / Lisa Yasmin Asseck and Christopher Grefen -- Ras recruitment system (RRS) for the identification and characterization of protein-protein interactions / Ami Aronheim -- Bacterial adenylate cyclase-based two-hybrid system compatible with gateway࣬oning / Macy G. Olson, Megan Goldammer, Emilie Gauliard, Daniel Ladant, and Scot P. Ouellette -- Analyses of protein interaction networks using computational tools / Shaowei Dong and Nicholas J. Provart -- Options and considerations when using a yeast one-hybrid system / Jared A. Sewell and Juan I. Fuxman Bass -- Screening arrayed libraries with DNA and protein baits to identify interacting proteins / Roc⩯ S⡮chez-Montesino and Luis Oñate-Sánchez -- Construction of Arabidopsis transcription factor ORFeome collections and identification of protein-DNA interactions by high-throughput yeast one-hybrid screens / S. Earl Kang, Ghislain Breton, and Jose L. Pruneda-Paz -- Transcription factor-centered yeast one-hybrid assay / Xiaoyu Ji, Liuqiang Wang, Dandan Zang, and Yucheng Wang -- Yeast three-hybrid system for protein interactions / Franziska Glass and Mizuki Takenaka -- Yeast Three-Hybrid System for Screening RNA-Binding Proteins in Plants / Sung Ki Cho and David J. Hannapel -- Using yeast hybrid system to identify proteins binding to small molecules / You Wang, David S. Letham, Peter C. L. John, and Ren Zhang -- Shuttling of entire libraries from an entry vector to a destination vector of the Gateway System / Alexander Heyl -- Bimolecular fluorescence complementation with improved gateway-compatible vectors to visualize protein-protein interactions in plant cells / Shino Goto-Yamada, Kazumi Hikino, Mikio Nishimura, Tsuyoshi Nakagawa, and Shoji Mano -- Bimolecular fluorescence complementation to visualize protein-protein interactions in human cells based on gateway cloning technology / Adriana Lepur and Oliver Vugrek -- KISS : a mammalian two-hybrid method for in situ analysis of protein-protein interactions / Delphine Masschaele, Sarah Gerlo, Irma Lemmens, Sam Lievens, and Jan Tavernier -- Coimmunoprecipitation of interacting proteins in plants / Alfonso Mu䮯z and M. Mar Castellano -- Analysis of protein-protein interaction by Co-IP in human cells / Zhenyuan Tang and Yoshinori Takahashi -- Tandem affinity purification of protein complexes from Arabidopsis cell cultures / Marta Garc⩡-Le⯮, Elisa Iniesto, and Vicente Rubio -- Transient transactivation studies in Nicotiana benthamiana leaves / Pilar Lasierra and Salom⥠Prat -- Use of the chromatin immunoprecipitation technique for in vivo identification of plant protein-DNA interactions / Jos⥠A. Jarillo, Dorota N. Komar, and Manuel Pi䮥iro -- Chromatin immunoprecipitation for identification of protein-DNA interactions in human cells / Bj⫠Ditlev Larsen, Martin RMadsen, Ronni Nielsen, and Susanne Mandrup
Online resource; title from PDF title page (SpringerProtocols, viewed June 4, 2018)
Link Print version: Two-hybrid systems : methods and protocols 9781493978700
Subject Yeast two-hybrid assay -- Laboratory manuals
Two-Hybrid System Techniques
Electronic books
Laboratory Manuals
Alt Author Oñate-Sánchez, Luis. editor
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