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Author DeKosky, Brandon, author
Title Decoding the antibody repertoire : high throughput sequencing of multiple transcripts from single b cells / by Brandon DeKosky
Imprint Cham : Springer International Publishing : Imprint: Springer, 2017
book jacket
Descript 1 online resource (xxviii, 87 pages) : illustrations, digital ; 24 cm
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
text file PDF rda
Series Springer theses, 2190-5053
Springer theses
Note Background -- High-throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire -- In-Depth Determination and Analysis of the Human Paired Heavy and Light Chain Antibody Repertoire -- Paired VH:VL Analysis of Naive B Cell Repertoires and Comparison to Antigen-Experienced B Cell Repertoires in Healthy Human Donors -- Conclusions and Future Perspectives -- Appendices
This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening the door for the discovery of new antibodies and the investigation of adaptive immune responses to vaccines and diseases. By designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells, the author directly addresses the limitations to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. Previous methods for high-throughput immune repertoire sequencing have been unable to provide such information. The techniques developed in this thesis have enabled comprehensive investigation of human B-cell repertoires and have been applied for the rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease
Host Item Springer eBooks
Subject B cells -- Research
Immunoglobulins -- Analysis
Genetic Engineering
Human Genetics
Cell Biology
Biochemistry, general
Alt Author SpringerLink (Online service)
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