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Title Multi-parametric live cell microscopy of 3D tissue models / edited by Ruslan I. Dmitriev
Imprint Cham : Springer International Publishing : Imprint: Springer, 2017
book jacket
Descript 1 online resource (xii, 172 pages) : illustrations (some color), digital ; 24 cm
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
text file PDF rda
Series Advances in experimental medicine and biology, 0065-2598 ; 1035
Advances in experimental medicine and biology ; 1035
Note Current state-of-the-art 3D tissue models and their compatibility with live cell imaging -- Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation -- Quantitative live cell FLIM imaging in three dimensions -- Three-dimensional tissue models and available probes for multi-parametric live cell microscopy: a brief overview -- Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues -- Multi-parametric imaging of hypoxia and cell cycle in intestinal organoid culture -- Imaging of intracellular pH in tumor spheroids using genetically encoded sensor SypHer2 -- Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell -- Quantitative imaging of Ca2+ by 3D-FLIM in live tissues -- Live cell imaging of viscosity in 3D tumour cell models -- Live imaging of cell invasion using a multicellular spheroid model and lightsheet microscopy -- Raman imaging microscopy for quantitative analysis of biological samples
This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM) A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial
Host Item Springer eBooks
Subject Tissue culture -- Simulation methods
Life Sciences
Regenerative Medicine/Tissue Engineering
Biological Microscopy
Biomedical Engineering/Biotechnology
Biomedical Engineering
Spectroscopy and Microscopy
Alt Author Dmitriev, Ruslan I., editor
SpringerLink (Online service)
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