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Title Hydrocarbon and lipid microbiology protocols : biochemical methods / Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales, editors
Imprint Berlin : Springer, 2016
book jacket
Descript 1 online resource (x, 188 pages) : illustrations (some color)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
Series Springer protocols handbooks, 1949-2448
Springer protocols handbooks
Note Online resource; title from PDF title page (SpringerLink, viewed September 1, 2016)
This volume provides protocols for the biochemical analysis of hydrocarbon - and lipid - relevant products, cell components and activities of microbes that interact with hydrophobic compounds. It includes methods for the extraction, purification and characterisation of surface tension-reducing bioemulsifiers and biosurfactants that increase the surface area and hence bioavailability of hydrophobic substrates
Introduction -- Protocols for the isolation and analysis of lipopeptides and bioemulsifiers -- Protocols for the detection and chemical characterisation of microbial glycolipids -- Isolation of glycoprotein bioemulsifiers produced by marine bacteria -- Protocols for lipid extraction from wet algal biomass -- Isolation and characterization of lipid droplets from yeast -- Protocols for Isolation and Analysis of Polyhydroxyalkanoates -- Purification of lipid rafts from bacterial membranes -- Isolation and analysis of membrane phospholipids as biomarkers -- Protocols for purifying and characterizing integral membrane AlkB enzymes -- Protocols for structural and functional analysis of particulate methane monooxygenase from Methylocystis species strain Rockwell (ATCC 49242) -- Phenotype-based Identification of key enzymes for polycyclic aromatic hydrocarbon (PAH) metabolism from Mycobacteria using transposon mutagenesis and a PAH spray plate -- Microcalorimetry as a general technique to characterize ligand binding
Includes bibliographical references
Link Original 3662491354 9783662491355 (OCoLC)930997282
Subject Lipids -- Laboratory manuals
Hydrocarbons -- Analysis -- Laboratory manuals
Synthetic Biology
Systems Biology
Electronic books
Laboratory manuals. fast (OCoLC)fst01920776
Laboratory Manuals
Alt Author McGenity, Terry J., editor
Timmis, K. N., editor
Nogales, Balbina, editor
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