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書名 Sphingosine-1-phosphate : methods and protocols / edited by Alice Pébay, Kursad Turksen
出版項 New York, NY : Humana Press, [2018]
©2018
國際標準書號 9781493974139
1493974130
1493974122
9781493974122
國際標準號碼 10.1007/978-1-4939-7413-9
9781493974122
book jacket
版本 Second edition
說明 1 online resource (x, 182 pages) : illustrations
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
text file PDF rda
系列 Methods in molecular biology, 1940-6029 : volume 1697
Methods in molecular biology ; 1697
附註 Includes bibliographical references and index
Online resource; title from PDF title page (SpringerLink, viewed December 8, 2017)
Measuring Sphingosine-1-Phosphate/Protein Interactions with the Kinetic Exclusion Assay -- An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity -- Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells -- Measurement of Lysophosphatidic Acid and Sphingosine-1-Phosphate by Liquid Chromatography-Coupled Electrospray Ionization Tandem Mass Spectrometry -- Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples and Cell Lines -- A Clean-Up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule -- A Rapid Fluorescence Assay for Measuring Sphingosine-1-Phosphate Transporter Activity in Erythrocytes -- Analysis of S1P Receptor Expression by Uterine Immune Cells Using Standardized Multi-Parametric Flow Cytometry -- S1P Synergizes with Wall Shear Stress and Other Angiogenic Factors to Induce Endothelial Cell Sprouting Responses -- In Vitro Methods to Study the Modulation of Migration and Invasion by Sphingosine-1-Phosphate -- Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor -- Sphingosine-1-Phosphate (S1P) Signaling in Neural Progenitors -- Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation -- 3D Stacked Construct: A Novel Substitute for Corneal Tissue Engineering
This fully updated edition explores methods involving sphingosine-1-phosphate (S1P), a bioactive lysophospholipid which has become the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells. Here, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology. As a volume in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols, Second Edition is another key resource for scientists working in this fast-moving and dynamic field
鏈接 Printed edition: Sphingosine-1-phosphate : methods and protocols 9781493974122
主題 Sphingosine -- Laboratory manuals
Amines
Sphingosine
Electronic books
Electronic books
Alt Author Pébay, Alice, editor
Turksen, Kursad, editor
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