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書名 Proteases and cancer : methods and protocols / edited by Santiago Cal and Alvaro J. Obaya
出版項 New York, N.Y. : Humana Press : Springer, [2018]
國際標準書號 9781493975952
book jacket
說明 1 online resource (xv, 352 pages) : illustrations (some color)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
text file PDF rda
系列 Methods in molecular biology, 1940-6029 ; 1731
Methods in molecular biology ; 1731
附註 Includes bibliographical references and index
Dissecting degradomes : analysis of protease-coding genes / gel varez-Eguiluz, Ander D⩡z-Navarro, and Xose S. Puente -- Identification of protease cleavage sites and substrates in cancer by Carboxy-TAILS (C-TAILS) / Nestor Solis and Christopher M. Overall -- Identification of proteolytic cleavage sites of EphA2 by membrane type 1 matrix metalloproteinase on the surface of cancer cells/ Keiji Kikuchi, Hiroko Kozuka-Hata, Masaaki Oyama, Motoharu Seiki, and Naohiko Koshikawa -- Biotin-chasing assay to evaluate uPAR stability and cleavage on the surface of cells / Vladimir Leksa, Herbert B. Schiller, and Hannes Stockinger -- Determination of aconitase activity : a substrate of the mitochondrial lon protease / Pedro M. Quir⯳ -- Simple cell-based assay for the detection of surface protein shedding by rhomboid proteases / Angela Moncada-Pazos and Adam Graham Grieve -- Functional production of catalytic domains of human MMPs in Escherichia coli periplasm / Dong Hyun Nam, Ki Baek Lee, and Xin Ge -- Autophagy and proteases : basic study of the autophagic flux by western blot / varo F. Fern⡮dez -- Gel-based gelatin zymography to examine matrix metalloproteinase activity in cell culture / Aastha Chhabra and Vibha Rani -- Analysis of enzymatic activity of matrix metalloproteinase (MMP) by collagen zymography in melanoma / Vijay Walia and Yardena Samuels -- Measurement of protease activities using fluorogenic substrates/ Salvatore Santamaria and Hideaki Nagase -- Targeting the expression of cathepsin B using CRISPR/Cas9 system in mammalian cancer cells / Manu Gnanamony and Christopher S. Gondi -- Assessing the influence of a protease in cell migration using the barrier-migration assay / Tania Fontanil, Yamina Mohamedi, Santiago Cal, and varo J. Obaya -- Analysis of invasive activity of CAF spheroids into three dimensional (3D) collagen matrices / Mar⩡ geles Villaronga, Sa⵬ varez Teijeiro, Francisco Hermida-Prado, Marta Garz⯮-Arango, Victoria Sanz-Moreno, and Juana Mar⩡ Garc⩡-Pedrero -- Beyond the proteolytic activity : examining the functional relevance of the ancillary domains using tri-dimensional (3D) spheroid invasion assay / Erico Tosoni Costa and Anamaria Aranha Camargo -- Analyzing the type II transmembrane serine protease hepsin-dependent basement membrane remodeling in 3D cell culture / Shishir M. Pant, Denis Belitskin, Hanna Ala-Hongisto, Juha Klefstr语, and Topi A. Tervonen -- Evaluation of tumor vasculature using a syngeneic tumor model in wild-type and genetically modified mice / Francisco Javier Rodr⩧uez-Baena, Silvia Redondo-Garc⩡, Mar⩡ del Carmen Plaza-Calonge, Rub⥮ Fern⡮dez-Rodr⩧uez, and Juan Carlos Rodr⩧uez-Manzaneque -- 3D image analysis of the microvasculature in healthy and diseased tissues / varo Sah⵮-Espa䮯l, Cristina Clemente, and Alicia G. Arroyo -- Human tumor tissue-based 3D in vitro invasion assays / Pirjo ꁳtr语, Ritva Heljasvaara, Pia Nyberg, Ahmed Al-Samadi, and Tuula Salo -- Ear sponge assay : a method to investigate angiogenesis and lymphangiogenesis in mice / Maureen Van de Velde, Melissa Garc⩡-Caballero, Tania Durr⥬ Fr⥤⥲ic Kridelka, and Agnᥳ No襬 -- Cancer susceptibility models in protease-deficient mice / Alicia R. Folgueras, Sandra Freitas-Rodr⩧uez, Yaiza Espa䮯l, and Gloria Velasco -- Imaging proteolytic activities in mouse models of cancer / Anupama Pal and Alnawaz Rehemtulla -- Protease silencing to explore the molecular mechanisms of cancer and aging / Julia M. Fraile, Diana Campos-Iglesias, and Jos⥠M.P. Freije -- Analysis of somatic DNA methylation alterations of genes encoding cell surface metallopeptidases in colorectal cancer / Sergio Alonso, Beatriz Gonz⡬ez, Andreu Alib⥳, and Manuel Perucho -- Targeting deubiquitinases in cancer / Joseph S. Bednash and Rama K. Mallampalli -- Generation of highly selective MMP antibody inhibitors / Dong Hyun Nam and Xin Ge -- Strategies to target matrix metalloproteinases as therapeutic approach in cancer / Zoi Piperigkou, Dimitra Manou, Konstantina Karamanou, and Achilleas D. Theocharis
Online resource; title from PDF title page (SpringerProtocols, viewed January 10, 2018)
鏈接 Print version: Proteases and cancer. New York, N.Y. : Humana Press : Springer, ò018 9781493975945
主題 Proteolytic enzymes -- Laboratory manuals
Cancer -- Etiology -- Laboratory manuals
Peptide Hydrolases
Electronic books
Laboratory Manuals
Alt Author Cal, Santiago, editor
Obaya, Alvaro J., editor
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