| 說明 |
1 online resource |
| 系列 |
Methods in cell biology ; v. 172
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| 附註 |
Print version record |
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Intro -- Radiation Oncology and Radiotherapy Part A -- Copyright -- Contents -- Contributors -- Radiation therapy: An old dog learning new tricks -- Acknowledgments -- Conflict of interest -- References -- Chapter 1: Cytofluorometric assessment of cell cycle progression in irradiated cells -- 1. Introduction -- 2. Materials -- 2.1. Common disposables -- 2.2. Cells and reagents -- 2.3. Equipment -- 2.4. Software -- 3. Methods -- 3.1. Cell seeding and treatment (see Note 17) -- 3.2. BrdU pulsing -- 3.3. Fixation, permeabilization and DNA denaturation -- 3.4. Staining -- 3.5. Flow cytometry acquisition -- 3.6. Data analysis -- 4. Notes -- 5. Concluding remarks -- Acknowledgments -- Competing interests -- References -- Chapter 2: Cytofluorometric assessment of acute cell death responses driven by radiation therapy -- 1. Introduction -- 2. Materials -- 2.1. Common disposables -- 2.2. Cells and reagents -- 2.3. Equipment -- 2.4. Software -- 3. Methods -- 3.1. Cell culture and treatments (see Note 21) -- 3.2. Sample collection -- 3.3. DiOC6(3)/PI co-staining -- 3.4. FITC-AnnV/PI co-staining -- 3.5. Acquisition -- 3.6. Gating and data analysis -- 4. Notes -- 5. Concluding remarks -- Acknowledgments -- Conflicts of interest -- References -- Chapter 3: Assessment of lipid peroxidation in irradiated cells -- 1. Introduction -- 2. Materials -- 2.1. Disposables -- 2.2. Reagents -- 2.3. Cell lines -- 2.4. Equipment -- 2.5. Software -- 3. Methods -- 3.1. Cell cultures -- 3.2. Cell irradiation -- 3.3. BODIPY 581/591 C11 staining -- 3.4. Flow cytometry -- 3.5. Data analysis -- 3.6. Concluding remarks -- 3.7. Notes -- Acknowledgments -- Disclosures -- References -- Chapter 4: Biochemical, genomic, and epigenomic profiling of isolated cancer cell lines micronuclei -- 1. Introduction -- 2. Materials (see note 1) |
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2. Material -- 2.1. Disposables -- 2.2. Equipment -- 2.3. Reagents -- 2.4. Biological material -- 3. Method -- 3.1. Cell culture -- 3.2. Compound preparation -- 3.3. Cell seeding and treatment -- 3.4. Image acquisition and analysis -- 3.5. Cell features extraction and data analysis -- 4. Notes -- 5. Concluding remarks -- Acknowledgments -- Conflict of interest -- References -- Chapter 10: RT-PCR-assisted quantification of type I IFN responses in irradiated cancer cells -- 1. Introduction -- 2. Materials -- 2.1. Common disposables -- 2.2. Cells and reagents -- 2.3. Equipment -- 3. Methods -- 3.1. Cell culture and treatments (see Note 9) -- 3.2. RNA extraction (see Note 19) -- 3.3. RT-PCR (see Note 32) -- 3.4. Data analysis -- 4. Notes -- 5. Concluding remarks -- Acknowledgments -- Competing interests -- References -- Chapter 11: ELISA-based quantification of type I IFN secretion by irradiated cancer cells -- 1. Introduction -- 2. Material -- 2.1. Disposables -- 2.2. Cells and reagents -- 2.3. Equipment -- 2.4. Software -- 3. Method -- 3.1. Cell culture, treatment and sample collection -- 3.2. Enzyme-linked immunosorbent assay (ELISA) -- 3.2.1. Reagent preparation -- 3.2.2. Procedure -- 3.2.3. Data analysis -- 3.3. Bead-based multiplex immunoassays -- 3.3.1. Reagent preparation -- 3.3.2. Procedure -- 3.3.3. Data acquisition with a flow cytometer and analysis -- 4. Concluding remarks -- Conflict of interest statement -- References -- Chapter 12: Assessment of hypoxia by pimonidazole staining following radiotherapy -- 1. Introduction -- 1.1. Hypoxia in tumors -- 1.2. Methods to measure hypoxia -- 1.3. Pimonidazole HCl -- 2. Materials -- 2.1. Common disposables -- 2.2. Equipment -- 2.3. Reagents -- 3. Methods -- 3.1. Establishment of B16F10 melanoma tumors and radiotherapy treatment |
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3.2. Pimonidazole administration, tumor collection and OCT preservation -- 3.3. Pimonidazole staining and fluorescence microscopy -- 4. Notes -- 5. Concluding remarks -- Disclosures -- References |
| 鏈接 |
Original 0323899498
9780323899499
(OCoLC)1331705957
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Print version: Radiation oncology and radiotherapy. Part A.
9780323899499
(OCoLC)1338669357
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| 主題 |
Radiotherapy -- Technological innovations
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Cancer -- Radiotherapy -- Technological innovations
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Radiotherapy
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Radiation Oncology
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Electronic books
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| Alt Author |
Sato, Ai, editor
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