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001 EBC340619
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007 cr cnu||||||||
008 200713s1998 xx o ||||0 eng d
020 9780080528090|q(electronic bk.)
020 |z9780124806108
035 (MiAaPQ)EBC340619
035 (Au-PeEL)EBL340619
035 (CaPaEBR)ebr10254644
035 (CaONFJC)MIL127948
035 (OCoLC)437208893
040 MiAaPQ|beng|erda|epn|cMiAaPQ|dMiAaPQ
050 4 QH212.E4M38 1999
082 0 570/.28/25
100 1 Maunsbach, Arvid B
245 10 Biomedical Electron Microscopy :|bIllustrated Methods and
Interpretations
264 1 San Diego :|bElsevier Science & Technology,|c1998
264 4 |c©1999
300 1 online resource (569 pages)
336 text|btxt|2rdacontent
337 computer|bc|2rdamedia
338 online resource|bcr|2rdacarrier
505 0 Front Cover -- BIOMEDICAL ELECTRON MICROSCOPY -- Copright
Page -- FOREWORD -- PREFACE -- ACKNOWLEDGMENTS -- CHAPTER
1. MICROGRAPH INTERPRETATION -- 1. Classical Preparation
Method -- 2. Low Temperature Approach -- 3. A Common Test
Specimen -- 4. Detection of Objects -- 5. Identification
of Artifacts -- 6. Analysis of Geometry -- 7. Biological
Identification -- 8. Biological Diversity -- 9. Analysis
of Dynamics: Endocytosis -- 10. Analysis of Dynamics:
Synthesis -- 11. Comparison of Methods -- 12. Variations
in Magnifications -- 13. Interpretation Difficulties --
14. Diagnostic Pathology -- CHAPTER 2. FIXATIVES -- 1.
Osmium Tetroxide and Glutaraldehyde at Low Magnification -
- 2. Osmium Tetroxide and Glutaraldehyde at High
Magnification -- 3. Glutaraldehyde Concentration:
Perfusion Fixation -- 4. Glutaraldehyde Concentration:
Immersion Fixation -- 5. Long Fixation Times -- 6.
Formaldehyde-Glutaraldehyde Combinations -- 7. Potassium
Permanganate, Picric Acid, and Ruthenium Red -- 8. Lead
Salts and Tannic Acid -- 9. Uranyl Acetate Postfixation --
10. Tannic Acid-Uranyl Acetate Variations -- 11. Osmium
Tetroxide-Potassium Ferrocyanide -- 12. Osmium Tetroxide
Artifacts -- 13. Glutaraldehyde Artifacts -- CHAPTER 3.
FIXATIVE VEHICLE -- 1. Absence and Presence of Buffer --
2. Comparison of Buffers -- 3. Osmolality of Perfusion
Fixatives -- 4. Effects of Osmolality on Cell Shape -- 5.
Effects of Osmolality on Cell Organelles -- 6. Adjustment
of Osmolality with Sucrose -- 7. Colloid Osmotic Pressure:
Low Magnification -- 8. Colloid Osmotic Pressure: High
Magnification -- 9. Phosphate Buffer Precipitate --
CHAPTER 4. FIXATIVE APPLICATION -- 1. Perfusion-Fixation
versus Immersion-Fixation -- 2. Perfusion-Fixation with
Pressure Control -- 3. Fixation by Dripping in Vivo -- 4.
Immersion-Fixation -- 5. Variability within the Tissue
505 8 6. Unsuccessful Perfusion-Fixation -- 7. Superficial
Tissue Damage -- 8. Early Postmortal Changes -- 9. Late
Postmortal Changes -- 10. Influence of Biopsy Method --
11. Microwave Treatment -- CHAPTER 5. DEHYDRATION AND
EMBEDDING -- 1. Stepwise versus Direct Dehydration -- 2.
Prolonged Dehydration in Ethanol -- 3. Prolonged
Dehydration in Acetone -- 4. Inert Dehydration -- 5.
Choice of Intermediate Solvent -- 6. Epon, Araldite, and
Vestopal: Unstained Sections -- 7. Epon, Araldite, and
Vestopal: Stained Sections -- 8. Different Brands of Epoxy
Resins -- 9. Spurr and LR White -- 10. Embedding of
Isolated Cells -- CHAPTER 6. FREEZING AND LOW-TEMPERATURE
EMBEDDING -- 1. Plunge Freezing -- 2. Contact Freezing of
Unfixed Tissue -- 3. Contact Freezing of Fixed Tissue --
4. High-Pressure Freezing -- 5. Freeze-Substitution in
Methanol/Uranyl Acetate -- 6. Freeze-Substitution in
Osmium Tetroxide Acetone -- 7. Progressive Lowering of
Temperature Embedding in Lowicryl -- CHAPTER 7. SUPPORT
FILMS -- 1. Surface Topography -- 2. Stability of Film or
Section -- 3. Holey Films -- 4. Thick and Thin Support
Films -- 5. Folds in Support Film -- 6. Defects in Formvar
Films -- 7. Common Contaminants -- 8. Volatile
Contamination -- CHAPTER 8. ULTRAMICROTOMY -- 1.
Correlation of Light and Electron Microscopy -- 2. Section
Thickness: Low Magnification -- 3. Section Thickness: High
Magnification -- 4. Section Thickness: Half-Micron Section
-- 5. Determination of Section Thickness -- 6. Folds in
the Section -- 7. Collection of Sections -- 8. Surface
Topography of Sections -- 9. Knife Scratches -- 10.
Mottling and Flaking -- 11. Worn Glass Knives -- 12.
Transmitted Vibrations -- 13. Vibrations and Knife Marks -
- 14. Selective Chatter -- 15. Compression -- 16. Holes
and Deformations -- 17. Contamination during Microtomy --
18. Extraction during Sectioning
505 8 19. Cryoultramicrotomy: Survey Sections -- 20. Collection
of Cryosections -- 21. Thickness of Cryosections -- 22.
Staining of Cryosections -- 23. Defects in Cryosections --
CHAPTER 9. SECTION-STAINING -- 1. Lead Citrate Staining --
2. Uranyl Acetate Staining -- 3. Enhanced Section Staining
-- 4. Effects of Grid Storage -- 5. Section Exposed to
Electron Beam -- 6. Effect of Electron Beam -- 7. Lead-
Staining Granularity -- 8. Contamination -- 9. Block-
Staining Precipitate -- 10. Removal of Contamination --
CHAPTER 10. MICROSCOPY -- 1. Resolving Power -- 2. Through
-Focus Series: Hole and Latex Particle -- 3. Through-Focus
Series: Myelin Sheath -- 4. Through-Focus Series: Cells --
5. Minimum Contrast Focusing -- 6. Wobbler Focusing -- 7.
Accelerating Voltages 20-100 kV -- 8. Accelerating
Voltages 80-200 kV -- 9. Unsaturated Electron Beam -- 10.
Condenser Apertures -- 11. Objective Aperture -- 12.
Through-Focus Series: Astigmatism -- 13. Image Distortion
-- 14. Chromatic Aberration -- 15. Mechanical Instability
-- 16. Specimen Drift versus Astigmatism -- 17. Focus
Drift -- 18. Electrical Instabilities -- 19. Contamination
in the Electron Beam -- 20. Radiation Damage -- 21.
Radiation Damage and Contamination -- 22. Low-Dose
Exposure -- 23. Spectroscopic Imaging: Thin Film -- 24.
Spectroscopic Imaging: Thick Section -- 25. Spectroscopic
Imaging: Carbon -- 26. Spectroscopic Imaging: Calcium --
27. Spectroscopic Imaging: Contrast Changes -- 28.
Cryoelectron Microscopy: Na,K-ATPase Crystals -- 29.
Defects in Cryoelectron Micrographs -- CHAPTER 11. IMAGE
RECORDING -- 1. Exposure Time -- 2. Over/Underexposure --
3. Effects of Development -- 4. Exposure Dose Adjustment -
- 5. Primary Magnification -- 6. Damage to Negatives -- 7.
Damage to Wet Negatives -- 8. Film/Imaging Plate/Charge-
Coupled Device (CCD) Camera -- 9. Enlarged Digital
Recordings
505 8 10. Variation in Electron Dose -- 11. Corrections of CCD
Camera -- CHAPTER 12. PHOTOGRAPHIC AND DIGITAL PRINTING --
1. Photographic Paper of Different Grades -- 2. Multigrade
Paper -- 3. Exposure and Development -- 4. Enlargement of
Micrograph Details -- 5. Objective Lens in Enlarger -- 6.
Focusing of Enlarger -- 7. Intermediate Diapositive -- 8.
Errors in Photographic Printing -- 9. Retouch -- 10.
Comparison of Printers: Low Magnification -- 12. Pixel
Size at Printing -- CHAPTER 13. NEGATIVE STAINING -- 1.
Negative Staining Methods -- 2. Properties of Support Film
-- 3. Comparison of Stains -- 4. Thickness of Stain -- 5.
Concentration of Specimen -- 6. Deformation of Specimen --
7. Radiation Damage -- CHAPTER 14. AUTORADIOGRAPHY -- 1.
Undeveloped Emulsion -- 2. Developed Emulsion -- 3.
Resolution -- 4. Quantitation -- 5. Preparatory Defects --
CHAPTER 15. CYTOCHEMISTRY -- 1. Influence of Fixation --
2. Preincubation Treatment -- 3. Appearance of Reaction
Product -- 4. Composition of Incubation Medium -- 5.
Cytochemical Resolution -- 6. Unspecific Staining -- 7.
Extraction of Reaction Product -- CHAPTER 16.
IMMUNOCYTOCHEMISTRY -- 1. Fixation of Sensitive Antigens -
- 2. Fixation of Insensitive Antigens -- 3. Comparison of
Embedding Media -- 4. Influence of Preincubation Solutions
-- 5. Comparison of Primary Antibodies -- 6. Dilution of
Primary Antibody -- 7. Quantitation of Gold Particles --
8. Controls -- 9. Comparison of Gold Probes -- 10.
Amplification of Gold Particles -- 11. Section Staining --
12. Resolution -- 13. Background Labeling -- 14. Antigen
Retrieval by Etching -- 15. Antigen Retrieval with Sodium
Dodecyl Sulfate -- 16. Double Labeling -- 17.
Immunonegative Staining -- 18. Freeze-Fracture Replica
Labeling -- 19. Preembedding Labeling -- 20. Semithin
Light Microscopic Sections -- CHAPTER 17. FREEZE
FRACTURING AND SHADOWING
505 8 1. Shadowing of DNA Molecules -- 2. Shadowing of Protein
Molecules -- 3. Freeze-Fractured Membrane Faces -- 4.
Thickness of Replica: Low Magnification -- 5. Thickness of
Replica: High Magnification -- 6. Rotary Shadowing -- 7.
Complementary Replicas and Stereo Images -- 8. Ice
Crystals and Etching -- 9. Quick-Freeze Deep Etching --
10. Identification of Transport Molecules -- 11.
Contamination -- 12. Plastic Distortion -- 13. Replica
Defects -- CHAPTER 18. SAMPLING AND QUANTITATION -- 1.
Calibration of Magnification -- 2. Sampling and Object
Variability -- 3. Sampling of Pellets: Differential
Centrifugation -- 4. Sampling of Pellets: Gradient
Centrifugation -- 5. Micrograph Montages -- 6. Automated
Digital Montages -- 7. Resolution of Digital Montages --
8. Measurements on Digital Images -- 9. Stereological
Grids -- 10. Cycloid Test System -- CHAPTER 19. IMAGE
PROCESSING -- 1. Digital Contrast Changes -- 2. Processing
of Scanned Image -- 3. Translational Image Enforcement --
4. Averaging of Macromolecular Assemblies -- 5. Rotational
Image Enforcement -- 6. Photographic versus Computer
Averaging -- 7. Fourier Correction of Section Chatter --
8. Removal of Image Defects -- 9. Scientific Fraud:
Removal of Objects -- 10. Scientific Fraud: Manipulation
of Labeling -- CHAPTER 20. THREE-DIMENSIONAL
RECONSTRUCTIONS -- 1. Comparison between Transmission and
Scanning Electron Microscopy -- 2. Serial Sectioning -- 3.
Large Three-Dimensional Objects -- 4. Tilting of Section
Cell Nucleus -- 5. Tilting of Section" Nuclear Envelope --
6. Helical Structures -- 7. Computer-Analyzed Helices --
8. A Three-Dimensional Model of Na, K-ATPase -- APPENDIX:
PRACTICAL METHODS -- 1. Fixation -- 2. Dehydration and
Embedding -- 3. Low Temperature Embedding -- 4. Support
Films -- 5. Ultramicrotomy -- 6. Section Staining -- 7.
Microscopy and Image Recording -- 8. Photographic Work
505 8 9. Negative Staining
520 This comprehensive reference illustrates optimal
preparation methods in biological electron microscopy
compared with common methodological problems. Not only
will the basic methodologies of transmission electron
microscopy like fixation, microtomy, and microscopy be
presented, but the authors also endeavor to illustrate
more specialized techniques such as negative staining,
autoradiography, cytochemistry, immunoelectron microscopy,
and computer-assisted image analysis. Authored by the key
leaders in the biological electron microscopy field
Illustrates both optimal and suboptimal or artifactual
results in a variety of electron microscopy disciplines
Introduces students on how to read and interpret electron
micrographs
588 Description based on publisher supplied metadata and other
sources
590 Electronic reproduction. Ann Arbor, Michigan : ProQuest
Ebook Central, 2020. Available via World Wide Web. Access
may be limited to ProQuest Ebook Central affiliated
libraries
650 0 Electron microscopy -- Technique.;Medical microscopy --
Technique
655 4 Electronic books
700 1 Afzelius, Björn A
700 1 Afzelius, Bjorn A
776 08 |iPrint version:|aMaunsbach, Arvid B.|tBiomedical Electron
Microscopy : Illustrated Methods and Interpretations|dSan
Diego : Elsevier Science & Technology,c1998|z9780124806108
856 40 |uhttps://ebookcentral.proquest.com/lib/sinciatw/
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