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Title Cryptosporidium [electronic resource] : Methods and Protocols / edited by Jan R. Mead, Michael J. Arrowood
Imprint New York, NY : Springer New York : Imprint: Humana, 2020
book jacket
Edition 1st ed. 2020
Descript XI, 407 p. 93 illus., 71 illus. in color. online resource
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
text file PDF rda
Series Methods in Molecular Biology, 1064-3745 ; 2052
Methods in Molecular Biology ; 2052
Note Cryptosporidium Diagnostic Assays: Microscopy -- Cryptosporidium Diagnostic Assays: Molecular Detection -- Detection of Cryptosporidium Recovered From Large-Volume Water Samples Using Dead-End Ultrafiltration -- Cryptosporidium Oocyst Purification Using Discontinuous Gradient Centrifugation -- Measuring Cryptosporidium Serologic Responses by Multiplex Bead Assay -- Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii -- Cryptosporidium Genotyping for Epidemiology Tracking -- Use of Markers to Determine Cryptosporidium Genotypes for Epidemiology Tracking and Detection -- Preparation of Cryptosporidium DNA for Whole Genome Sequencing -- Accessing Cryptosporidium Omic and Isolate Data Via -- A Novel Method to Silence Genes in Cryptosporidium -- Use of miRNAs to Study Host Cell-Parasite Interactions -- Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9 -- Mouse Models for Use in Cryptosporidium Infection Studies and Quantification of Parasite Burden Using Flow Cytometry, qPCR, and Histopathology -- Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics -- Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water -- High-Content Screening for Cryptosporidium Drug Discovery -- High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR -- In Vitro Culture of Cryptosporidium parvum Using Hollow Fiber Bioreactor: Applications for Simultaneous Pharmacokinetic and Pharmacodynamic Evaluation of Test Compounds -- In Vitro Culture of Cryptosporidium parvum Using Stem Cell-Derived Intestinal Epithelial Monolayers -- Two and Three Dimensional Bioengineered Human Intestinal Tissue Models for Cryptosporidium
This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon. The chapter "Accessing Cryptosporidium Omic and Isolate Data via" is available open access under a Creative Commons Attribution 4.0 International License via
Host Item Springer eBooks
Link Printed edition: 9781493997473
Printed edition: 9781493997497
Printed edition: 9781493997503
Subject Parasitology
Infectious diseases
Infectious Diseases.
Alt Author Mead, Jan R. editor. Editor.
Arrowood, Michael J. editor. Editor.
SpringerLink (Online service)
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