LEADER 00000nam  2200313   4500 
001    AAI1433551 
005    20100917125247.5 
008    100917s2006    ||||||||||||||||| ||eng d 
020    9780542601910 
035    (UMI)AAI1433551 
040    UMI|cUMI 
100 1  Fernandez, Pravina P 
245 10 Selection of an aptamer against surface exposed targets on
       Yersinia pestis 
300    131 p 
500    Source: Masters Abstracts International, Volume: 44-05, 
       page: 2218 
500    Adviser: Kenneth D. Clinkenbeard 
502    Thesis (M.S.)--Oklahoma State University, 2006 
520    Scope and method of study. Yersinia pestis is a CDC 
       'category A' bioterrorism agent. This study attempts to 
       select an aptamer to detect the presence of LOS and F1 
       antigen on Y. pestis . Aptamer selection by Systematic 
       Evolution of Ligand by Exponential Enrichment is an 
       iterative process of binding, partitioning, amplification 
       and strand separation 
520    Findings and conclusions. The Lipid A as well as the core 
       polysaccharide of LOS was found unfavorable for aptamer 
       binding. Purified F1 antigen was considered a suitable 
       target for aptamer selection. Our partitioning method 
       using electrodialysis and eight rounds of selection 
       resulted in thirty nine clones from Round 8 with a 
       consensus motif --GTGAG---GTTG--. However, binding assays 
       were unsuccessful due to non-specificity of Round 8 to F1 
       antigen, and hence additional four rounds of SELEX are 
       being carried out. The aptamer thus selected might be used
       in in vitro diagnostic assays to detect F1 antigen of 
       Yersinia pestis 
590    School code: 0664 
650  4 Biology, Microbiology 
690    0410 
710 2  Oklahoma State University 
773 0  |tMasters Abstracts International|g44-05 
856 40 |uhttp://pqdd.sinica.edu.tw/twdaoapp/servlet/