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Title Microglia : methods and protocols / edited by Bertrand Joseph, Department of Oncology-Pathology, Karolinska Institutet, Cancer Centrum Karolinska, Stockholm, Sweden, José Luis Venero, Departamento de Bioquimica y Biologia Molecular, Facultad de Farmacia, Universidad de Sevilla, Sevilla, Spain, Instituto de Biomedicina de Sevilla, Hospital Universitaria, Sevilla, Spain
Imprint New York : Humana Press, [2013]
©2013
book jacket
Descript 1 online resource (xii, 350 p.) : illustrations (some color)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
Series Methods in molceular biology, 1940-6029 ; 1041
Methods in molecular biology ; 1041
Note Part I Overview of microglia biology -- Brief overview of multitalented microglia / Bertrand Joseph and José Luis Venero -- Part II Isolation and culture of microglia -- Cell culturing of human and murine microglia cell lines / Johanna Rodhe -- Microglia isolation from adult mouse brain / Jae-Kyung Lee and Malú G. Tansey -- Preparation of primary microglia cultures from postnatal mouse and rat brains / Tomas Deierborg -- Isolation of murine postnatal brain microglia for phenotypic characterization using magnetic cell separation technology / Ashley S. Harms and Malú G. Tansey -- Isolation and culture of adult human microglia within mixed glial cultures for functional experimentation and high-content analysis / Amy M. Smith ... [et al.] -- Part III Depletion and transduction of microglia -- Depletion of microglia from primary cellular cultures / Lorena Pont-Lezica, Sabrina Colasse, and Alain Bessis -- Lentiviral transduction of cultured microglia / Takahiro Masuda ... [et al.] -- Part IV Analysis of microglial cytokine production -- Microglial activation : measurement of cytokines by flow cytometry / Deepak Kumar Kaushik and Anirban Basu -- In situ hybridization of cytokine mRNA using alkaline phosphatase-labelled oligodeoxynucleotide probes / Bettina Clausen, Christina Fenger, and Bente Finsen -- Use of meso-scale discovery to examine cytokine content in microglia cell supernatant / Miguel A. Burguillos -- Part V Analysis of microglia activation -- Analysis of microglial production of reactive oxygen and nitrogen species / Urte Neniskyte and Guy C. Brown -- Quantification of active caspase-3 and active caspase-8 in microglia cells / Edel Kavanagh -- Quantification of microglial phagocytosis by a flow cytometer-based assay / Refik Pul, Kandiyil Prajeeth Chittappen, and Martin Stangel -- Quantification of microglial proliferation and apoptosis by flow cytometry / Alicia A. Babcock, Martin Wirenfeldt, and Bente Finsen -- Fluorescence imaging of intracellular Ca2+, Na+, and H+ in cultured microglia / Tom Schilling and Claudia Eder -- Patch clamp protocols to study ion channel activity in microglia / Tom Schilling and Claudia Eder -- Part VI Analysis of microglia polarization -- Studying M1 and M2 states in adult microglia / Sadanand M. Gaikwad and Michael T. Heneka -- Isolating, culturing, and polarizing primary human adult and fetal microglia / Bryce A. Durafourt ... [et al.] -- Part VII Co-culture systems to analysis microglia interactions with other cell types -- Understanding microglia-neuron cross talk : relevance of the microglia-neuron cocultures / Fernando G. Correa, Miriam Hernangómez, and Carmen Guaza -- Preparation of rodent primary cultures for neuron-glia, mixed glia, enriched microglia, and reconstituted cultures with microglia / Shih-Heng Chen, Esteban A. Oyarzabal, and Jau-Shyong Hong -- Part VIII Analysis of microglia functions in vivo -- Microglia detection by enzymatic histochemistry / Beatriz Almolda, Berta González, and Bernardo Castellano -- Tomato lectin histochemistry for microglial visualization / Nàdia Villacampa ... [et al.] -- Immunohistochemical detection of microglia / Rocío M. de Pablos, Ana M. Espinosa-Oliva, and Antonio J. Herrera -- Intrathecal Infusion of Microglia Cells / Takahiro Masuda ... [et al.] -- Intracranial injection of LPS in rat as animal model of neuroinflammation / Ana M. Espinosa-Oliva, Rocío M. de Pablos, and Antonio J. Herrera -- Analyses of microglia effector function using CX3CR1-GFP knock-in mice / Jenny A. Garcia, Sandra M. Cardona, and Astrid E. Cardona -- In vivo two-photon microscopy of microglia / Satoru Kondo and Shigeo Okabe -- Use of confocal microscopy in the study of microglia in a brain metastasis model / Manuel Sarmiento
Includes bibliographical references and index
Description based on print version record
Link Print version: Microglia. New York : Humana Press ; Springer, c2013 9781627035194 (OCoLC)839397551
Subject Microglia
Microglia
Electronic books
Alt Author Joseph, Bertrand. Editor.
Venero, José Luis. Editor.
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