LEADER 00000nam  2200409   4500 
001    AAI1506338 
005    20121203125325.5 
008    121203s2011    ||||||||||||||||| ||eng d 
020    9781267177292 
035    (UMI)AAI1506338 
040    UMI|cUMI 
100 1  Patel, Ronak 
245 10 Characterization of multifunctional biodegradable-
       biocompatible ICG-loaded nanoparticles and their 
       feasibility for prostate cancer imaging and phototherapy 
300    102 p 
500    Source: Masters Abstracts International, Volume: 50-04, 
       page: 2520 
500    Adviser: Hanli Liu 
502    Thesis (M.S.)--The University of Texas at Arlington, 2011 
520    Prostate cancer is the most common male cancer in the 
       United States, and the second leading cause of cancer 
       death in men. The conventional diagnostic procedures use 
       an invasive approach to collect the biopsy tissue samples.
       Also, treatments currently used include radiotherapy, 
       chemotherapy and surgical removal of the prostate 
       associated with high risks of surgical complications. 
       Among all the imaging modalities, Near Infrared (NIR) 
       optical imaging could be a possible candidate for 
       minimally invasive diagnosis of prostate cancer with 
       avoidance of harmful radiation and relatively low cost. 
       This motivation brings the development of multifunctional 
       polymeric (di-lactide-co-glycolic acid) (PLGA) 
       Nanoparticles encapsulating indocyanine green (ICG) which 
       acts as exogenous NIR contrast and thermal agent specific 
       to prostate cancer 
520    The objective of this study was to evaluate and 
       characterize multi-functionality of biodegradable--
       biocompatible ICG loaded PLGA nanoparticles as thermal 
       ablation agent for cancer therapy and as an optical 
       contrast agent for prostate cancer imaging. The important 
       parameters for particle characterization such as particle 
       size, polydispersity index and ICG loading efficiency were
       investigated. For optical characterization of 
       nanoparticles, the excitation--emission matrix, absorption
       spectra and fluorescence lifetime were determined. The 
       photostability study on the optical parameters reveals the
       stability of PLGA--ICG Nanoparticles (PIN) in aqueous 
       medium. The human dermal fibroblast (HDF) cell viability 
       study demonstrated the cytocompatibility of PIN. 
       Successful cellular uptake of R11 peptide conjugated PIN 
       with prostate cancer (PC3) cells was obtained. Absorption 
       capability of PIN was also explored as a thermal ablation 
       agent through a phantom study and ex vivo tissue phantom 
       studies. Moreover, results of thermal ablation through the
       phantom study showed an increase of ∼ 10--12°C in 
       temperature, which is sufficient to create local 
       hyperthermia to treat cancer. The application of PIN as an
       exogenous contrast was investigated through an ex vivo 
       tissue and in vivo animal model to examine PIN's ability 
       for enhancing imaging contrast for prostate tumor 
       detection by multi spectral imaging. The results showed 
       great promises, suggesting possible application of PIN 
       towards diagnostic imaging and therapy 
590    School code: 2502 
650  4 Engineering, Biomedical 
650  4 Nanoscience 
650  4 Health Sciences, Radiology 
650  4 Health Sciences, Oncology 
690    0541 
690    0565 
690    0574 
690    0992 
710 2  The University of Texas at Arlington.|bBiomedical 
       Engineering 
773 0  |tMasters Abstracts International|g50-04 
856 40 |uhttp://pqdd.sinica.edu.tw/twdaoapp/servlet/
       advanced?query=1506338