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Title Pseudomonas methods and protocols / edited by Alain Filloux, Juan-Luis Ramos
Imprint New York, NY : Humana Press, 2014
book jacket
Descript 1 online resource (xviii, 816 pages) : illustrations (some color)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
Series Methods in Molecular Biology, 1064-3745 ; 1149
Methods in molecular biology ; 1149
Note Gene transfer : transduction / Emanuela Frangipani -- Gene transfer : transformation/electroporation / Frédéric Cadoret, Chantal Soscia, and Romé Voulhoux -- Gene transfer : conjugation / Thibault G. Sana, Aurélie Laubier, and Sophie Bleves -- Pseudomonas bacteriophage isolation and production / Joana Azeredo, Sanna Sillankorva, and Diana P. Pires -- Genotyping methods / Burkhard Tümmler -- Drug susceptibility testing by dilution methods / Katy Jeannot and Patrick Plésiat -- Plate-based assay for swimming motility in Pseudomonas aeruginosa / Dae-Gon Ha, Sherry L. Kuchma, and George A. O'Toole -- Plate-based assay for swarming motility in Pseudomonas aeruginosa / Dae-Gon Ha, Sherry L. Kuchma, and George A. O'Toole -- Motility assay : twitching motility / Lynne Turnbull and Cynthia B. Whitchurch -- Qualitative and quantitative assays for flagellum- mediated chemotaxis / José Antonio Reyes Darias ... [et al.] -- Microscopic analysis : morphotypes and cellular appendages / Olga Zaborina, John Alverdy, Megha Shah, and Yimei Chen -- Determination of lipolytic enzyme activities / Karl-Erich Jaeger and Filip Kovacic -- Elastinolytic and Proteolytic Enzymes / Efrat Kessler and Mary Safrin -- In vitro assays to monitor the activity of Pseudomonas aeruginosa type III secreted proteins / Stephanie L. Rolsma and Dara W. Frank -- Cell fractionation / Bérengère Ize, Véronique Viarre, and Romé Voulhoux -- Characterization of molecular interactions using isothermal titration calorimetry / Tino Krell ... [et al.] -- Proteomic analysis / Pascal Cosette and Thierry Jouenne -- Membrane proteomics of Pseudomonas aeruginosa / Zofia Magnowska, Isabel Hartmann, Lothar Jänsch, and Dieter Jahn -- Construction of Pseudomonas aeruginosa two-hybrid libraries for high-throughput assays / Sophie de Bentzmann and Christophe Bordi -- Biosensors for qualitative and semiquantitative analysis of quorum sensing signal molecules / Matthew Fletcher ... [et al.] -- LC-MS/MS quantitative analysis of quorum sensing signal molecules / Catharine A. Ortori ... [et al.] -- LC/MS/MS-based quantitative assay for the secondary messenger molecule, c-di-GMP / Yasuhiko Irie and Matthew R. Parsek -- Metabolic footprinting : extracellular metabolomic analysis / Volker Behrends, Huw D. Williams, and Jacob G. Bundy -- Pyoverdine and pochelin measurements / Françoise Hoegy, Gaetan L. A. Mislin, and Isabelle J. Schalk -- Measurement of phenazines in bacterial cultures / Suzanne E. Kern and Dianne K. Newman -- Extraction and measurement of NAD(P)+ and NAD(P)H / Suzanne E. Kern, Alexa Price-Whelan, and Dianne K. Newman -- Cyanide measurements in bacterial culture and sputum / Chandrika Goh Nair, Ben Ryall, and Huw D. Williams -- Monitoring iron uptake by siderophores / Françoise Hoegy and Isabelle J. Schalk -- Exopolysaccharide quantification / Irina Sadovskaya -- Liquid chromatography/mass spectrometry for the identification and quantification of rhamnolipids / Ahmad Mohammad Abdel-Mawgoud, François Lépine, and Eric Déziel -- LPS quantitation procedures/ Joseph S. Lam, Erin M. Anderson, and Youai Hao -- Monitoring lectin interactions with carbohydrates / Sophie de Bentzmann, Annabelle Varrot, and Anne Imberty -- Mining the pseudomonas genome / Geoffrey L. Winsor and Fiona S. L. Brinkman -- Identification of bacterial small RNAs by RNA sequencing / María Gómez-Lozano ... [et al.] -- Gene amplification and qRT-PCR / Cerith Jones and Alain Filloux -- Standard european vector architecture (SEVA) plasmid toolkit / Gonzalo Durante-Rodríguez, Víctor de Lorenzo, and Esteban Martínez-García -- Chromosomal integration of transcriptional fusions / Rafael Silva-Rocha and Víctor de Lorenzo -- Method to capture large DNA fragments from genomic DNA / Geneviève Ball, Alain Filloux, and Romé Voulhoux -- Transposon mutagenesis / Hemantha D. Kulasekara -- Site-directed mutagenesis and gene deletion using reverse genetics / Daniela Muhl and Alain Filloux -- Signature-tagged mutagenesis / Irena Kukavica-Ibrulj and Roger C. Levesque -- Construction of a Pseudomonas aeruginosa genomic DNA library / Christophe Bordi -- Strategy for genome sequencing analysis and assembly for comparative genomics of Pseudomonas Genomes / Julie Jeukens ... [et al.] -- Promoter fusions with optical outputs in individual cells and in populations / Ilaria Benedetti and Victor de Lorenzo -- Chromatin immunoprecipitation for ChIP-chip and ChIP-seq / Sebastian Schulz and Susanne Häussler -- Transcriptional analysis of Pseudomonas aeruginosa infected Caenorhabditis elegans / Ashleigh MacKenzie ... [et al.] -- Methods for studying biofilm formation : flow cells and confocal laser scanning microscopy / Tim Tolker-Nielsen and Claus Sternberg -- Biofilm formation in the 96-well microtiter plate / Barbara M. Coffey and Gregory G. Anderson -- Methods for studying biofilm dispersal in Pseudomonas aeruginosa / Nicolas Barraud ... [et al.] -- Pseudomonas aeruginosa PA14 pathogenesis in Caenorhabditis elegans / Natalia V. Kirienko ... [et al.] -- Assessing Pseudomonas aeruginosa virulence using a nonmammalian host : Dictyostelium discoideum / Geneviève Filion and Steve J. Charette -- Assessing pseudomonas virulence with nonmammalian host : Galleria mellonella / Gudrun Koch ... [et al.] -- Assessing pseudomonas virulence with the nonmammalian host model : Arabidopsis thaliana / Regina L. Baldini, Melissa Starkey, and Laurence G. Rahme -- Assessing Pseudomonas aeruginosa persister/antibiotic tolerant cells / Ronen Hazan ... [et al.] -- Assessing pseudomonas virulence with nonmammalian host : zebrafish / María A. Llamas and Astrid M. van der Sar -- Assessing pseudomonas virulence with a nonmammalian host : Drosophila melanogaster / Samantha Haller, Stefanie Limmer, and Dominique Ferrandon -- Assessing pseudomonas virulence using host cells / Iwona Bucior, Cindy Tran, and Joanne Engel -- Assessing Pseudomonas aeruginosa virulence and the host response using Murine models of acute and chronic lung infection / Irena Kukavica-Ibrulj ... [et al.] Bragonzi -- Assessing pseudomonas virulence using mammalian models : acute infection model / Antje Munder and Burkhard Tümmler -- Burn mouse models / Henrik Calum, Niels Høiby, and Claus Moser
Includes index
Description based on online resource; title from PDF title page (SpringerLink, viewed June 6, 2014)
Subject Pseudomonas -- Laboratory manuals
Life Sciences
Microbiology
Bacteriology
Electronic books
Alt Author Filloux, Alain, editor
Ramos, Juan-Luis, 1956- editor
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