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Title Dendritic cell protocols / edited by Stephen P. Robinson and Andrew J. Stagg ; foreword by Stella C. Knight
Imprint Totowa, N.J. : Humana Press, 2001
book jacket
Descript 1 online resource (xvii, 470 pages) : illustrations
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
Series Methods in molecular medicine ; 64
Methods in molecular medicine ; 64
Note Includes bibliographical references and index
pt. 1. Isolation of dendritic cells from animal tissues -- Isolation of dendritic cells from mouse lymph nodes / D. Gabrilovich -- Isolation of mouse spleen dendritic cells / A.J. Stagg -- Isolation of mouse thymic dendritic cells / F. Anju⥲e -- Isolation of dendritic cells from rat intestinal lymph and spleen / G. MacPherson -- Isolation, enrichment, and culture of murine epidermal Langerhans cells / F. Koch -- Murine intestinal dendritic cell isolation / P. Pavli -- Identification and isolation of rodent lung dendritic cells / P.A. Stumbles -- Isolation and propagation of mouse liver-derived dendritic cells / L. Lu -- pt. 2. Isolation and characterization of human dendritic cells -- Identification and immunophenotypic analyses of a peripheral blood dendritic cells / S.P. Robinson -- Purification of dendritic cells from peripheral blood / S. Patterson -- Isolation of human tonsillar dendritic cells / P.U. Cameron -- Isolation of dendritic cells from human afferent lymph / C.U. Brand -- Isolation of human skin dendritic cells by in vitro migration / C.D. Richters -- Human intestinal dendritic cell isolation / P. Pavli -- Isolation of human lung dendritic cells / M.M. van den Heuvel -- Identification and isolation of synovial dendritic cells / A.R. Pettit -- pt. 3. Derivation of animal dendritic cells from progenitor cells -- Generation of murine bone-marrow-derived dendritic cells / A. Maroof -- Generation of dendritic cells from rat bone marrow / T. Powell -- Derivation of murine dendritic cells from thymic precursors / K. Lucas -- pt. 4. Generation of murine dendritic cell lines -- Generation of mouse dendritic cell lines / S. Citterio -- Generation of Langerhans cell-like dendritic cells from murine fetal skin / T. Jakob -- Derivation of dendritic cell lines from mouse skin / A. Takashima -- pt. 5. Generation of human dendritic cells from progenitor cells -- Propagation of human dendritic cells in vitro / C. Caux -- Generation of dendritic cells from lymphoid precursors / A. Galy -- Dendritic cell generation from highly purified CD14+ monocytes / W.F. Pickl -- Generation of blood-derived human dendritic cells for antitumor immunotherapy / M.F. Gilliet -- pt. 6. Aspects of dendritic cell function -- Chemotaxis of in vitro cultured human dendritic cells / W. Luini -- Adhesion of dendritic cells to endothelia / K.A. Brown -- Transendotherlial migration and reverse transmigration of in vitro cultured human dendritic cells / G. Bianchi -- In vivo assays of Langerhans cells migration / M. Cumberbatch -- In vitro migration of rat dendritic cells / K. Matsuno -- Antigen uptake by dendritic cells / C. Caux -- Uptake of antigen-antibody complexes by human dendritic cells / N.A. Fanger -- Immunoelectron microscopy of antigen processing in dendritic cells / M.J. Kleijmeer -- Purification and characterization of MHC class II containing organelles in mouse bone-marrow-derived dendritic cells / P. Pierre -- Detection of intracellular cytokines in dendritic cells / P. Kelleher -- Adenoviral-vector-mediated gene transfer to dendritic cells / W. Song -- Stimulation of anti-HIV-1 cytotoxic T lymphocytes by dendritic cells / X. Huang
Print version record
It is now apparent that dendritic cells not only play important roles in the body's immune system through their complex interactions with T cells, B cells, and other cell types, but also possess distinct functional attributes that enable them to assume different roles in that system. In Dendritic Cell Protocols, Stephen P. Robinson, MD, PhD, and Andrew Stagg, PhD, have brought together a wide range of time-proven methods for studying these so-called "veiled" cells. Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide variety of tissues or from various progenitor cell populations. Other methods describe in step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation. Variant methods that have been successful in other laboratories have been included to expand experimental possibilities. In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeutics. State-of-the-art and highly practical, Dendritic Cell Protocols provides both experienced and novice investigators with powerful tools to illuminate the complex biology of these foundation cells-cells that shape the evolution of acquired immune responses, play important roles in innate immunity, and promise the development of powerful new immunotherapeutics
Link Dendritic cell protocols 9780896035843 0896035840 (DLC) 00047235
Subject Dendritic cells -- Laboratory manuals
Dendritic Cells -- physiology
Dendritic Cells -- cytology
Electronic books
Alt Author Robinson, Stephen P. (Stephen Paul)
Stagg, Andrew J
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