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Author Riccio, Paolo
Title Molecules : Nucleation, Aggregation and Crystallization - Beyond Medical and Other Implications
Imprint Singapore : World Scientific Publishing Company, 2009
©2009
book jacket
Descript 1 online resource (365 pages)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
Note Contents -- Preface -- 1. From Kuru to Nucleation, Aggregation, Polymerization and Crystallization in Biology and Medicine D. Carleton Gajdusek -- Molecular Casting -- Infectious amyloid nucleants -- Can we preserve biological specificity of antibodies, antigens, pheronemes, receptors, transmitters, ion channels and enzymes in organic molecular casts or atomic moulds? -- Dermatoglyphic preserving of biological specificity -- Fossils show accurate speciation in paleobotany and paleozoology -- Osmium shading in electron microscopy reveals details of molecular structure at nanometric distances -- Mineral nucleants for crystal growth in outer space -- What makes a diamond hold together? -- Twinning of minerals -- Industrial viruses and "ice nine" -- Amyloid enhancing factors are scrapie infectious amyloid nucleants -- Any β-pleated polymeric assembly as a two-dimensional sheet or as a fibril may act as a heteronucleant for different amyloidogenic proteins -- Synthesis of prion-like infectious nucleants -- Biological macromolecules all interact strongly with SiO2, the most common solid mineral on the surface of Earth. Montmorillonite clay deposits cause delayed neurodegenerative diseases -- Nucleation is speleology -- Nucleation in extragalactic space -- References -- 2. Gels Mimicking Antibodies in Their Selective Recognition of Proteins and Its Potential Use for Protein Crystallization Jan Sedzik, Nasim Ghasemzadeh, Fred Nyberg and Stellan Hjertén -- Introduction -- Experimental Details -- Materials -- Preparation of a ribonuclease-specific gel -- Preparation of a gel for specific adsorption of human growth hormone -- Can a bed be designed with selectivity for more than one protein? -- The degree of selectivity of the adsorption -- Selective bed with higher flow rate -- Results and Discussion -- The selectivity of the artificial gel antibodies
Do some protein molecules become attached covalently to the gel matrix? -- What conclusions can be drawn from the finding that (bio-)affinity methods do not always give the selectivity theoretically expected? -- Classification of chromatographic techniques in terms of the number of bonds between the solute and the stationary phase and the strength of these bonds -- Some comments on the mechanism of selective recognition -- Artificial gel antibodies for detection of biomarkers -- Selectivity, protein capacity and imprinting of bioparticles -- Potential applications -- The design of a chromatographic bed for removal of impurifying proteins ("negative purification") -- Forthcoming studies -- Conclusions -- Acknowledgments -- References -- 3. Bioinformatics of Myelin Membrane Proteins Gunnar von Heijne and Jan Sedzik -- Introduction -- Definition of Bioinformatics -- The Basis of Biological Membranes -- Biological Membranes - Protein/Lipid Ratio -- Basic Bioinformatics -- Focus on the Myelin Membrane -- Water Soluble Versus Integral Myelin Proteins -- Myelin basic protein -- P2 protein -- P0 protein -- PMP22 protein -- PLP protein -- MAG -- MOG -- Summary -- Acknowledgment -- References -- 4. Biomolecular Mass Spectrometry: Applications to Proteins and Peptides Leopold L. Ilag and Gianluca Maddalo -- Introduction -- Proteomics -- Techniques and strategies -- Peptide mass fingerprinting -- De novo sequencing -- Post-translational and Chemical Modifications -- MALDI Imaging -- MS in structural biology -- Fibroblast growth factor (FGF) and fibroblast growth factor receptor (FGFR) -- RNA polymerase -- Ribosome -- Protein-lipid interactions -- Conclusion -- Acknowledgments -- References -- 5. Myelin: A One-Dimensional Biological "Crystal" for X-Ray and Neutron Scattering Hideyo Inouye and Daniel A. Kirschner -- Introduction
Electron Density Profile on an Absolute Scale -- Chemical Interpretation of the Electron Density and Neutron Scattering Length Density Profiles -- Method -- Model calculation -- Conclusion -- Acknowledgments -- References -- 6. Two-Dimensional Crystallization of Biological Macromolecules Hans Hebert -- Introduction -- Why Two-Dimensional Crystals? -- Electron Crystallography: Initial Characterization -- How to Make 2D Crystals of Proteins -- Membrane proteins -- Soluble proteins -- Properties of 2-D crystals -- Symmetry -- Size -- Order -- Flatness -- Stacking, multilayers -- Electron Crystallography: Data Collection and Processing -- Specimen preparation and temperature -- Electron diffraction -- Images -- Data processing -- 2D or 3D Crystallization? -- Summary -- Acknowledgments -- References -- 7. Crystallization of Proteins: Principles and Methods Lata Govada -- Introduction -- Principles of Crystallization -- The crystallization phase diagram -- The solubility curve -- The supersolubility curve -- Precipitation zone -- Nucleation zone -- Metastable zone -- Construction of Supersolubility Curve -- Crystallization Methodologies -- Batch crystallization -- Modified microbatch -- Diffusion methods -- Vapor diffusion -- Hanging drop -- Sitting drop -- Sandwich drop -- Liquid-liquid interface diffusion -- Granada crystallization box® (GCB) -- Dialysis -- Bulk dialysis -- Microdialysis -- Conclusions -- Acknowledgments -- References -- 8. The Role of Oil in Protein Crystallization Naomi E. Chayen -- Introduction -- The Microbatch Techniques -- The mechanism of crystallization under oil -- The application of oil to improve vapour diffusion experiments -- The contribution of oil to the control of heterogeneous nucleation -- Cleanliness of trials -- Effect of surface contact -- Protection of crystallization samples and crystals by the oil
Limitation of Crystallizing Under Oil -- Application of organic molecules as precipitants and/or additives -- Harvesting and mounting of crystals -- Crystallization of membrane proteins under oil -- Summary -- Acknowledgment -- References -- 9. Introduction to Crystallization of Fine Chemicals and Pharmaceuticals Åke C. Rasmuson -- Introduction -- Fundamentals -- Solubility and Supersaturation -- Nucleation -- Crystal Growth -- The Product -- Productivity -- Solid phase -- Particle size -- Crystal shape -- Product purity -- The Process -- The role of agitation -- Control of the product crystal mean size -- Conclusions -- References -- 10. Myelin Basic Protein, A Saucy Molecule With High Responsiveness to the Environment or Just an Unusual Membrane Protein? Paolo Riccio -- Introduction -- Myelin Basic Protein: What Is It and What Does It Do? -- The IUPs -- MBP Ligands and Functions -- Post-Translational Modifications of MBP -- Is MBP a Natively Unfolded Protein? -- The absence of structure in the acid-extracted molecule -- MBP as a membrane protein: The discovery of lipid-bound MBP -- Partitioning of MBP in the Myelin Membrane -- Incorporation of myelin basic protein in liposomes made of myelin lipids -- Concluding Remarks -- Acknowledgments -- References -- 11. The Use of a Magnetic Field and Magnetic Force as a Means to Improve the Quality of Protein Crystals Mitsuo Ataka -- Introduction -- Theoretical Background -- Magnetic field orientation -- Magnetic force -- Technical Background -- Generation of magnetic fields by various magnets -- Superconducting magnets for supplying uniform magnetic force -- Modes of operation of various magnets -- Historical Background -- Our Studies on Magnetic Orientation -- Protein crystals grown in a magnetic field exhibit orientation -- Sedimenting crystals -- Other Studies on Magnetic Orientation of Protein Crystals
Other Uses of Magnetic Orientation -- Other Possible Mechanisms Through Which Homogeneous Magnetic Fields May Contribute to Quality Improvement -- Studies on Crystal Perfection in Homogeneous Magnetic Fields -- Magnetic Force -- Our studies on the use of a magnetic force -- Convection Suppression or Damping in the Presence of Magnetic Force -- Summary -- References -- 12. Interactive Crystallomic Jan Sedzik -- Introduction -- Experimental Procedure - An Example -- Pre-crystallization assumptions -- Chemicals and crystallization set-up -- Rationale and design of the crystallization trials -- Algorithm -- Results -- Discussion -- Summary -- Acknowledgments -- References -- 13. Virtual Molecule: P0 Myelin Glycoprotein. I. Homology Modeling and Prediction of the Secondary and Tertiary Structure Jan Pawel Jastrzebski and Jan Sedzik -- Introduction -- Methods and Tools for Homology Prediction -- Amino Acid Sequence - A Basic Input for Modeling -- Construction and Analysis of a Three-Dimensional Atomic Model of P0 Glycoprotein -- The P0 extracellular domain - Soluble part -- The P0 transmembrane segment is 21 amino residues long -- The P0 intracellular part -- Theoretical model of P0 -- Hydrophobicity and Electrostatic Potential of P0 Protein -- Structural Effects of Breaking the Disulfide Bridge -- Medical Implications - Autoimmune Epitopes of Myelin P0 Protein -- Summary and Discussion -- Acknowledgments -- References -- 14. In meso Approaches to Membrane Protein Crystallization Valentin I. Gordeliy and Ekaterina S. Moiseeva -- Introduction -- Membrane Protein Crystallization: A Standard Approach -- In mesoMembrane Protein Crystallization -- Cubic phase crystallization -- Crystallization in the sponge phase -- Crystallization from vesicles -- Crystallization from bicelles -- Towards a General Method of Membrane Protein Crystallization -- Summary
Acknowledgments
Key Features:Suitable as an advanced text for graduate students and non-experts alikeContains sufficiently detailed text to capture the interest of curious readersServes as an introduction to explore the research literature in the fieldIncludes contributions by prominent authors such as Nobel Laureate Carlton Gajdusek
Description based on publisher supplied metadata and other sources
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2020. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
Link Print version: Riccio, Paolo Molecules: Nucleation, Aggregation And Crystallization: Beyond Medical And Other Implications Singapore : World Scientific Publishing Company,c2009 9789812832641
Subject Macromolecules.;Proteins.;Nucleation.;Aggregation (Chemistry);Crystallization
Electronic books
Alt Author Sedzik, Jan
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