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Title Gene correction : methods and protocols / edited by Francesca Storici
Imprint New York : Humana Press, 2014
book jacket
Descript 1 online resource (xvi, 463 pages) : color illustrations
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
Series Methods in molecular biology ; 1114
Methods in molecular biology ; 1114
Note RecTEPsy-mediated recombineering in Pseudomonas syringae / Bryan Swingle - Genome manipulations with bacterial recombineering and site-specific integration in Drosophila / Yi Zhang, William Schreiner, and Yikang S. Rong -- Multiple genetic manipulations of DT40 cell line / Akira Motegi and Minoru Takata -- Gene targeting of human pluripotent stem cells by homologous recombination / Sara E. Howden and James A. Thomson -- Methods for the assessment of ssODN-mediated gene correction frequencies in muscle cells / Carmen Bertoni -- Small fragment homologous replacement (SFHR) : sequence-specific modification of genomic DNA in eukaryotic cells by small DNA fragments/ Andrea Luchetti, Arianna Malgieri, and Federica Sangiuolo -- Preparation and application of triple helix forming oligonucleotides and single strand oligonucleotide donors for gene correction / Md. Rowshon Alam ... [et al.] -- Triplex-mediated genome targeting and editing / Faisal Reza and Peter M. Glazer -- Targeting piggyBac transposon integrations in the human genome / Daniel L. Galvan, Claudia S. Kettlun, and Matthew H. Wilson -- Gene targeting in human-induced pluripotent stem cells with adenoviral vectors / Kohnosuke Mitani -- Enhanced gene targeting of adult and pluripotent stem cells using evolved adeno-associated virus / Melissa A. Bartel and David V. Schaffer -- Lentiviral vectors encoding zinc-finger nucleases specific for the model target locus HPRT1 / Laetitia P.L. Pelascini and Manuel A.F.V. Gonçalves -- Designing and testing the activities of TAL effector nucleases / Yanni Lin, Thomas J. Cradick, and Gang Bao -- Bacterial one-hybrid system to isolate homing endonuclease variants with altered DNA target specificities / Rakesh Joshi and Frederick S. Gimble -- Design and analysis of site-specific single-strand nicking endonucleases for gene correction / Michael J. Metzger and Michael T. Certo -- CRISPR-Cas-mediated targeted genome editing in human cells / Luhan Yang ... [et al.] -- RNA-guided genome editing of mammalian cells / Neena K. Pyzocha ... [et al.] -- Nuclease-mediated double-strand break (DSB) enhancement of small fragment homologous recombination (SFHR) gene modification in human- induced pluripotent stem cells (hiPSCs) / R. Geoffrey Sargent, Shingo Suzuki, and Dieter C. Gruenert -- AAV-mediated gene editing via double-strand break repair / Matthew L. Hirsch and R. Jude Samulski -- Genetic modification stimulated by the induction of a site- specific break distant from the locus of correction in haploid and diploid yeast Saccharomyces cerevisiae / Samantha Stuckey and Francesca Storici -- Southern blot protocol to detect chimeric nuclease- mediated gene repair / Céline J. Rocca ... [et al.] -- High-throughput cellular screening of engineered nuclease activity using the single-strand annealing assay and luciferase reporter / Thomas J. Cradick, Christopher J. Antico, and Gang Bao -- Unbiased method for detection of genome-wide off- target effects in cell lines treated with zinc finger nucleases / Cory R. Lindsay and David B. Roth -- Identification of off-target cleavage sites of zinc finger nucleases and TAL effector nucleases using predictive models / Eli J. Fine, Thomas J. Cradick, and Gang Bao -- Method for retinal gene repair in neonatal mouse / Marilyn Dernigoghossian ... [et al.] -- In utero delivery of oligodeoxynucleotides for gene correction / Lingzhi Cai ... [et al.] -- Portal vein delivery of viral vectors for gene therapy for hemophilia / Alexandra Sherman ... [et al.] -- Gene correction of induced pluripotent stem cells derived from a murine model of x-linked chronic granulomatous disorder / Sayandip Mukherjee and Adrian J. Thrasher -- Efficient transduction of hematopoietic stem cells and its potential for gene correction of hematopoietic diseases / Dolly Thomas and Gustavo Mostoslavsky
Includes bibliographical references and index
Gene correction is a technology that gives us the tools for both repairing and mutating DNA, for discovering gene functions and for engineering new genetic variants. Gene Correction: Methods and Protocols provides a user friendly, detailed and up-to-date collection of strategies and methodologies utilized for generating specific sequence changes in the DNA of cells in the laboratory, while also tackling the major problems that the field of gene correction faces. This volume brings together many experts in the field of gene correction to disclose a wide and varied array of specific gene correction protocols for engineering mutations in DNA, for delivering correcting DNA to target cells, and for improving the accuracy and safety of the gene correction process. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Gene Correction: Methods and Protocols seeks to serve scientists of all backgrounds interested in the area of gene targeting/recombination/therapy
Description based on print version record
Link Print version: Gene correction 1627037608 (OCoLC)859042291
Subject Genetic recombination
Genetic regulation
Mutation (Biology)
Electronic books
Alt Author Storici, Francesca, editor of compilation
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