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Author Simón, Carlos
Title Stem Cells in Reproductive Medicine : Basic Science and Therapeutic Potential
Imprint New York : Cambridge University Press, 2013
book jacket
Edition 3rd ed
Descript 1 online resource (216 pages)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
Note Intro -- Contents -- Contributors -- Preface -- 1 Genetics of germline formation -- Introduction -- Primordial germ-cell specification -- BMP4 -- BMP8B -- BMP2 -- FRAGILIS and STELLA -- BLIMP1/PRDM1 -- PRDM14 -- PGC migration -- KITL and c-KIT -- CXCL12 (SDF1), CXCR4, and E-cadherin -- Sex determination -- Male germline formation -- SRY -- SOX9 -- FGF9 and retinoic acid -- Female germline formation -- WNT4 -- RSPO1 -- -catenin -- FOXL2 -- DAX1 -- Retinoic acid -- Summary -- 2 Germ-cell differentiation from pluripotent cells -- Introduction -- Germline development in mammals -- Pregonadal development: Specification, migration, and colonization of gonadal crests -- Postgonadal development: Sex specification, meiosis, and germ-cell maturation -- Stem cells as a source of germ cells in vitro -- State of the art in germ-cell differentiation from mESCs -- State of the art in germ-cell differentiation from hESCs and hiPSCs -- Main challenges in germ-cell differentiation from pluripotent cells -- Meiosis and its molecular regulation -- Post-transcriptional regulation by RNA-binding proteins and small non-coding RNAs -- Concluding remarks and future applications -- 3 The male gamete -- Introduction -- When sperm cells are absent or fail -- Help wanted: Sperm donation in the era of ICSI -- Clinical indications and reproductive outcome -- What we learned from infertile male sperm -- Male infertility and defective sperm production -- Sperm defects related to sperm counts -- Sperm defects unrelated to sperm counts -- Spermatogonial stem cells to restore male fertility -- Molecular features of the functional spermatozoa -- Chromosomal content and genetic status -- Imprinting -- Sperm DNA integrity -- Oxidative stress defence in sperm -- Other molecular keys in sperm physiology -- Oxygen consumption ability
Information obtained from the application of massive analysis techniques to sperm -- Sperm lipidic profile -- Where are we now and where can we go: Conclusions -- 4 In vitro production of functional sperm from neonatal mouse testes -- Introduction -- Development of spermatogonial transplantation technique -- In vitro multiplication of spermatogonial stem cells -- In vitro spermatogenesis with organ culture method -- In vitro transplantation and culture method (IVTC method) -- Cryopreservation of SSCs and testis tissues -- Conclusion (future of clinical applications) -- Acknowledgment -- 5 Adult stem-cell population in the human testis -- Introduction -- SSC progenitors populate the testes during fetal development -- SSCs balance between self-renewal and differentiation -- The identity of SSCs is becoming elucidated -- SSCs are housed in specialized niches -- SSCs can survive, proliferate, and differentiate in vitro -- Spermatogonial stem cells might be pluripotent -- Fertility restoration after SSC loss -- Protocols have been developed to cryopreserve SSCs -- Spermatogonial stem-cell transplantation -- Testicular tissue grafting -- Contaminating malignant cells need to be removed -- Potential applications of SSCs in cell therapy -- SSCs as a source for regenerative medicine -- SSCs at the basis of transgenerational therapy -- Conclusive thoughts -- Acknowledgments -- 6 Meiotic recombination in human oocytes -- Meiosis is a sexually dimorphic process -- The basics of recombination: how crossovers are made -- Measuring recombination -- Retrospective analyses of recombination -- Linkage analysis -- Linkage disequilibrium (LD) mapping -- Centromere mapping -- Cytological analysis -- Analysis of crossover-associated proteins -- Analysis of chiasmata -- Recombination in the human female -- What we have learned from linkage studies
Watching recombination as it happens: cytological studies of pachytene oocytes -- Recombination and non-disjunction -- Summary -- 7 Gene expression dynamics during human embryonic development -- Introduction to human embryo development and reprogramming -- Induced pluripotency -- Significance of elucidating gene expression patterns during pre-implantation development extends to pluripotent stem-cell biology -- Gene expression in the oocyte-to-embryo transition -- A survey of human embryo development and gene expression -- Transcription profiling of the human oocyte -- Cell cycle drivers and checkpoints in the human eight-cell embryo -- Validation of gene expression in a large set of oocytes and embryos at six developmental stages -- Analysis of single blastomeres from five- to eight-cell embryos -- Correlating molecular data with imaging data and developmental potential -- Imaging parameters prospectively diagnose embryo fate in in vitro experiments -- Embryo stage-specific gene expression -- Individual blastomeres show cell autonomy -- The promise of novel analytic tools -- Summary -- 8 Embryonic stem cells from blastomeres maintaining embryo viability -- Introduction -- ES cells via parthenogenesis -- Transdifferentiation or reprogramming -- Single blastomere-derived ES cells -- 9 Gamete generation from stem cells to avoid gamete donation and customized hESCs from blastomeres as the cellular insurance for the newborn: Will it ever be ethically acceptable? -- Introduction -- Gamete generation to avoid gamete donation -- Gamete generation from adult stem cells/iPS cells -- Gamete generation from embryonic stem cells -- Moral status of the human embryo -- Embryonic parents -- Informed consent -- "Non-medical" applications -- Conclusion -- Customized hESCs from blastomeres as the cellular insurance for the newborn -- Avoiding embryo destruction
The advantage of having an autologous stem-cell line in storage -- Efficiency -- The alternatives -- Conclusion -- 10 Amniotic fluid and placental membranes: Uunexpected sources of highly multipotent cells -- Introduction -- Development of gestational stem cells -- Isolation and characterization of amniotic fluid stem cells -- Multipotency of amniotic fluid stem cells -- Osteocytes -- Myocytes -- Adipocytes -- Endothelial cells -- Hepatocytes -- Chondrocytes -- Neuronal Cells -- Clinical application of amniotic fluid stem cells -- Bone regeneration -- Myocardial infarction -- Renal disease -- Neural regeneration -- Lung regeneration -- Blood and immune system regeneration -- Other clinical applications -- Conclusion -- 11 Adult stem cells in the human endometrium -- Introduction -- Regenerative capacity of human endometrium -- Endometrial stem/progenitor cell hypothesis -- Adult stem cells and their properties -- Endometrial epithelial stem/progenitor cells -- Cell cloning studies -- Label-retaining cells -- Endometrial mesenchymal stromal/stem cells -- Cell cloning studies -- Multilineage differentiation -- Label-retaining cells -- Side population (SP) cells -- Endometrial reconstitution in vivo -- Endometrial stem/progenitor cell markers -- Human endometrial MSC markers -- Menstrual blood stem/progenitor cells -- Sources of endometrial stem/progenitor cells -- Remnant fetal stem cells -- Circulating stem cells from the bone marrow -- Endometrial stem/progenitor cells: clinical perspective -- Cancer stem cells in endometrial cancer -- Endometriosis -- Adenomyosis -- Tissue-engineering applications -- Conclusions and future directions for human endometrial stem/progenitor cell research -- Acknowledgments -- 12 In utero hematopoietic cell transplantation -- Introduction -- Human clinical experience -- Animal experience with IUHCTx
Barriers to engraftment after IUHCTx -- Competitive advantage -- Space in hematopoietic niches -- Rejection of transplanted cells -- Conclusions -- 13 Bone-marrow stroma: A source of mesenchymal stem cells for cell therapy -- Mesenchymal stem cells (MSCs): general properties -- MSC markers -- Immunological properties of MSCs -- In vivo MSCs form a part of the cell microenvironment of bone marrow -- Therapeutic applications of MSCs -- Capacity of MSCs to differentiate towards various cell lineages -- MSCs in the treatment of GvH disease -- MSCs for improving HSC engraftment and preventing GvHD -- Role of MSCs in autoimmunity -- Conclusions and further directions -- Acknowledgments -- 14 Dedifferentiation, transdifferentiation, and reprogramming -- Introduction -- Different roads to changing cell potency -- Dedifferentiation -- Transdifferentiation -- Epigenetic memory -- Concluding remarks -- 15 The metabolic framework of pluripotent stem cells and potential mechanisms of regulation -- Introduction -- Metabolism and the maintenance of pluripotency -- Metabolism and the differentiation of pluripotent cells -- Reprogramming energy metabolism -- Regulation of ES cell metabolism by the culture environment -- Molecular regulation of metabolic pathways in pluripotent stem cells -- Conclusions -- Index
Stem Cells in Reproductive Medicine is essential reading for those keeping abreast of practical developments in this rapidly moving field
Description based on publisher supplied metadata and other sources
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2020. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
Link Print version: Simón, Carlos Stem Cells in Reproductive Medicine : Basic Science and Therapeutic Potential New York : Cambridge University Press,c2013 9781107034471
Subject Embryonic stem cells.;Reproductive health.;Human reproductive technology
Electronic books
Alt Author Pellicer, Antonio
Reijo Pera, Renee
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