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Title Advances and clinical practice in pyrosequencing [electronic resource] / editors Guohua Zhou, Qinxin Song
Imprint New York, NY : Humana Press ; Springer, c2016
book jacket
Descript 1 online resource (xiv, 407 p.) : ill
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
Series Springer protocols handbooks
Springer protocols handbooks
Note Preparation of single-stranded DNA for pyrosequencing by LATE-PCR / Huiyong Yang ... [et al.] -- Simplified protocol for preparing pyrosequencing templates based on LATE-PCR using whole blood as starting material directly / Yunlong Liu ... [et al.] -- Improvement of LATE-PCR to prepare pyrosequencing template / Qinxin Song ... [et al.] -- Pyrosequencing templates generated by nicking PCR products with nicking endonuclease / Qinxin Song ... [et al.] -- Pyrosequencing templates generated by asymmetric nucleic acid sequence-based amplification (Asymmetric-NASBA) / Huning Jia ... [et al.] -- Gel immobilization of acrylamide-modified single-stranded DNA template for pyrosequencing / Pengfeng Xiao ... [et al.] -- Multiplex PCR based on a universal biotinylated primer to generate templates for pyrosequencing / Zhiyao Chen ... [et al.] -- Novel pyrosequencing principle based on AMP-PPDK reaction for improving the detection limit / Guohua Zhou ... [et al.] -- Pyrosequencing chemistry coupled with modified primer extension reactions for quantitative detection of allele frequency in a pooled sample / Guohua Zhou ... [et al.] -- Gel-free SNP genotyping method based on pyrosequencing chemistry coupled with modified primer extension reactions directly from double-stranded PCR products / Guohua Zhou ... [et al.] -- Improvement of pyrosequencing to allow multiplex SNP typing in a pyrogram / Guohua Zhou ... [et al.] -- Improvement of pyrosequencing sensitivity by capturing free adenosine 5'-phosphosulfate with adenosine triphosphate sulfurylase / Haiping Wu ... [et al.] -- Using polymerase preference index to design imLATE-PCR primers for an efficient pyrosequencing / Qinxin Song ... [et al.] -- Preparation of thermal-stable biotinylated firefly luciferase and its application in pyrosequencing / Shuhui Zhu ... [et al.] -- Expression of thermostable recombinant Luciola lateralis luciferase and its function on setting up of heat-stable pyrosequencing system / Shu Xu ... [et al.] -- Expression and purification of ATP sulfurylase from Saccharomyces cerevisias in Escherichia coli and its application in pyrosequencing / Juan Luo ... [et al.] -- Characterization of recombinant Escherichia coli single-strand binding protein and its application in pyrosequencing / Jianping Wang ... [et al.] -- Expression of PPDK from Microbispora rosea subsp. aerata in E. coli and its application in pyrosequencing / Bingjie Zou, Qinxin Song, and Guohua Zhou -- Construction of 3-plex barcodes for differential gene expression analysis with pyrosequencing / Xiaodan Zhang ... [et al.] -- Development of pyrosequencing-based multiplex bioassay by designing barcodes encoded with artificially designed sequences / Zhiyao Chen ... [et al.] -- Quantitatively discriminating multiplexed LAMP products with pyrosequencing-based bio-barcodes / Chao Liang ... [et al.] -- Multiplex ligation-dependent sequence-tag amplification coupled with pyrosequencing for copy number variation (CNV) analysis / Haiping Wu ... [et al.] -- Pyrosequencer miniaturized with capillaries to deliver deoxynucleotides / Guohua Zhou ... [et al.] -- Pyrosequencing on acryl-modified glass chip / Huan Huang ... [et al.] -- Pyrosequencing on-chip based on a gel-based solid-phase amplification / Huan Huang ... [et al.] -- Prenatal diagnosis of trisomy 21 by quantitatively pyrosequencing heterozygotes using amniotic fluid as starting material of PCR / Hui Ye ... [et al.] -- Comparative gene expression analysis of breast cancer-related genes by multiplex pyrosequencing coupled with sequence barcodes / Qinxin Song ... [et al.] -- MicroRNA quantification by pyrosequencing with a sequence-tagged stem-loop RT primer / Hua Jing ... [et al.] -- Analysis of genetically modified organisms by pyrosequencing on a portable photodiode-based bioluminescence sequencer / Qinxin Song ... [et al.] -- Genotyping of pathogenic serotypes of S. suis with pyrosequencing / Huiyong Yang ... [et al.] -- Differential gene expression analysis of breast cancer by combining sequence-tagged reverse-transcription PCR with pyrosequencing / Xiaodan Zhang ... [et al.] -- Detection of avian influenza A virus by pyrosequencing / Weipeng Wang ... [et al.] -- Genotyping of alcohol dehydrogenase gene by pyrosequencing coupled with improved LATE-PCR using human whole blood as starting material / Zheng Xia ... [et al.] -- Pyrosequencing on a single cell / Qinxin Song ... [et al.]
Description based on online resource; title from PDF title page (SpringerLink, viewed February 10, 2016)
Includes bibliographical references and index
Subject Nucleotide sequence -- Methodology
Sequence Analysis, DNA -- methods
Electronic books
Alt Author Zhou, Guohua
Song, Qinxin
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