LEADER 00000nam  2200325   4500 
001    AAI3041102 
005    20080107105533.5 
008    080107s2001                        eng d 
020    9780493545202 
035    (UMI)AAI3041102 
040    UMI|cUMI 
100 1  Donaldson, Kim A 
245 10 Molecular evolution of a coding and a non-coding region of
       the mitochondrial genome of three transisthmian species of
       fishes (Centropomidae: Centropomus) 
300    202 p 
500    Source: Dissertation Abstracts International, Volume: 63-
       02, Section: B, page: 0694 
500    Major Professor:  Raymond R. Wilson, Jr 
502    Thesis (Ph.D.)--University of South Florida, 2001 
520    Fish divergence times are often inferred from DNA sequence
       data calibrated by rates derived from the mammalian fossil
       record, but it is not clear if mammalian mitochondrial DNA
       substitution rates are comparable enough to those of 
       fishes for correct inferences. From comparative analysis 
       of control region (ca. 1097 bases) and ND5/6 (890 bases) 
       sequences from geminate species of snook (Centropomus) 
       believed to have been separated by the Pliocene uplift of 
       the Isthmus of Panama, this dissertation seeks to 
       calibrate a fish divergence rate for the control region. 
       Additionally, the phylogenetic utility of the control 
       region and ND5/6 sequences are investigated. The most 
       plausible general control region divergence rate of 3.2% 
       per million years +/- 1.1% for centropomids was obtained 
       after applying a 1% per million years divergence rate to 
       transversion substitutions at third codon-positions of ND5
       /6 sequences to estimate the separation time of the fish 
       geminate species, rather than directly applying the time 
       suggested by the Pliocene uplift. A control region 
       divergence rate of 2.1 per million years appeared the most
       conceivable when only geminate species pairs were compared
       to each other. However, because a segment of one ND5/6 
       sequence might have been corrupted by some type of 
       interspecific recombination or introgression, the derived 
       rates should be interpreted carefully. The 1% per million 
       years rate is essentially a mammalian rate, so fish and 
       mammalian divergence rates appear comparable in the 
       studied mitochondrial DNA sequences. It appears that the 
       three centropomid control region domains have comparable 
       ability to resolve only the most closely related species. 
       The maximum likelihood method appeared the most robust 
       algorithm for analyzing the control region domains. Tests 
       with several tree-building methods showed using the entire
       control region data set more consistently provided an 
       accepted phylogeny rather than using any one domain 
590    School code: 0206 
590    DDC 
650  4 Biology, Genetics 
650  4 Biology, Zoology 
690    0369 
690    0472 
710 2  University of South Florida 
773 0  |tDissertation Abstracts International|g63-02B 
856 40 |uhttp://pqdd.sinica.edu.tw/twdaoapp/servlet/
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