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008 200713s2016 xx o ||||0 eng d
020 9781780407425|q(electronic bk.)
020 |z9781780407418
035 (MiAaPQ)EBC4742388
035 (Au-PeEL)EBL4742388
035 (CaPaEBR)ebr11298150
035 (OCoLC)960788734
040 MiAaPQ|beng|erda|epn|cMiAaPQ|dMiAaPQ
050 4 QR92.P59.P474 2017
082 0 628.3
100 1 Flemming, Hans-Curt
245 14 The Perfect Slime :|bMicrobial Extracellular Polymeric
Substances (EPS)
264 1 London :|bIWA Publishing,|c2016
264 4 |c©2016
300 1 online resource (336 pages)
336 text|btxt|2rdacontent
337 computer|bc|2rdamedia
338 online resource|bcr|2rdacarrier
505 0 Cover -- Copyright -- Contents -- Preface -- Chapter 1:
The perfect slime - and the "dark matter" of biofilms --
Abstract -- 1.1 What is 'Perfect'? -- 1.2 The Matrix:
Basis for the Emergent Properties of Biofilms -- 1.3 The
"Dark Matter of Biofilms" -- 1.3.1 What influences EPS
production and how can it be managed? -- 1.3.2 Where and
by which mechanisms are hydrophobic substances sorbed in
the matrix? -- 1.3.3 What are the mechanisms behind water
retention? -- 1.3.4 Can the permeability of biofouling
layer be engineered in membrane technology? -- 1.3.5 Which
are the interactions of the various EPS components? --
1.3.6 What is the function of complex EPS components? --
1.3.7 Which EPS components contribute to matrix stability
and how can we predict it? -- 1.4 Chaos and Function -
Self-Organization -- Acknowledgements -- References --
Chapter 2: EPS - a complex mixture -- Abstract -- 2.1
Introduction -- 2.2 Microbial Polysaccharide Composition -
- 2.3 Polysaccharide Structure -- 2.4 Physical Properties
-- 2.5 Polysaccharide Interactions -- References --
Chapter 3: The extracellular matrix - an intractable part
of biofilm systems -- Abstract -- 3.1 Introduction -- 3.2
Challenges -- 3.3 Biofilm Matrix Analysis -- 3.4 Biofilm
Matrix Constituents -- 3.4.1 Polysaccharides -- 3.4.2
Proteins -- 3.4.3 Amyloids -- 3.4.4 Extracellular nucleic
acids -- 3.4.5 Amphiphilic compounds -- 3.4.6 Membrane
vesicles -- 3.4.7 Refractory compounds -- 3.5 Bacterial
Extracellular Biology -- 3.6 Biofilm Matrix Functionality
-- 3.6.1 Architecture -- 3.6.2 Protection -- 3.6.3 Cryo-
and Osmo-protection -- 3.6.4 Sorption -- 3.6.5
Precipitation -- 3.6.6 Adhesion -- 3.6.7 Repellent --
3.6.8 Cohesion -- 3.6.9 Connectivity -- 3.6.10 Activity --
3.6.11 Surface-activity -- 3.6.12 Information -- 3.6.13
Competition -- 3.6.14 Nutrition -- 3.6.15 Motility --
3.6.16 Transportation
505 8 3.6.17 Communication -- 3.6.18 Conduction -- 3.6.19 Redox-
activity -- 3.6.20 Dispersion -- 3.7 Emerging Views of the
Biofilm Matrix -- References -- Chapter 4: The transition
from bacterial adhesion to the production of EPS and
biofilm formation -- Abstract -- 4.1 Introduction -- 4.2
The Transition from Bacterial Adhesion to Biofilm
Formation -- 4.3 Bacterial Surface Sensing and Cell Wall
Deformation -- 4.4 Methods to Study Bacterial Cell Wall
Deformation -- 4.4.1 Macroscopic bio-optical fluorescence
imaging -- 4.4.2 Atomic force microscopy -- 4.4.3 Surface
thermodynamic approach -- 4.5 Bacterial Surface Sensing
and the Role of EPS in Biofilms -- 4.5.1 Bacterial surface
sensing and EPS production -- 4.5.2 Role of EPS in
biofilms -- 4.5.2.1 EPS and resistance of biofilms against
mechanical attack -- 4.5.2.2 EPS and resistance of
biofilms against chemical attack -- 4.5.2.3 Lubricating
properties of EPS in biofilms -- 4.6 Methods to Study
Biofilm Composition and Structure -- 4.6.1 Microscopic
structure of biofilms -- 4.6.2 Composition of biofilms --
4.6.3 Viscoelastic properties of biofilm -- 4.6.4
Lubricating properties of biofilms with and without EPS --
4.7 Concluding Comments -- References -- Chapter 5:
Genetics and regulation of EPS formation in Pseudomonas
aeruginosa -- Abstract -- 5.1 Introduction -- 5.1.1
Biofilm formation by Pseudomonas aeruginosa -- 5.2 PSL
(Polysaccharide Synthesis Locus) -- 5.2.1 Psl composition
and structure -- 5.2.2 Psl synthesis -- 5.2.3
Transcriptional regulation of Psl -- 5.2.4 Post-
transcriptional regulation of Psl -- 5.3 PEL (Pellicle
Formation) -- 5.3.1 Pel composition and structure -- 5.3.2
Pel synthesis -- 5.3.3 Transcriptional regulation of Pel -
- 5.3.4 Post-transcriptional regulation of Pel -- 5.4
Alginate -- 5.4.1 Alginate composition and structure --
5.4.2 Alginate synthesis
505 8 5.4.3 Transcriptional regulation of alginate -- 5.4.4 Post
-transcriptional regulation of alginate -- 5.5 Conclusions
and Perspectives -- References -- Chapter 6: Amyloids - a
neglected child of the slime -- Abstract -- 6.1
Introduction -- 6.2 Visualization of Amyloids and their
Abundance in Biofilms -- 6.3 Only a Few Functional
Amyloids have been Characterized -- 6.3.1 Curli fimbriae -
- 6.3.1.1 Biological role -- 6.3.1.2 Biogenesis -- 6.3.1.3
Biotechnological applications -- 6.3.2 Functional amyloids
of Pseudomonas (Fap) -- 6.3.2.1 Biological role -- 6.3.2.2
Biogenesis -- 6.3.3 TasA from Bacillus -- 6.3.3.1
Biological role -- 6.3.3.2 Biogenesis -- 6.3.4 Other EPS-
associated functional amyloids -- 6.4 Isolation and
Characterization of Functional Amyloids -- 6.5 Concluding
Remark -- References -- Chapter 7: Bacterial
exopolysaccharides from unusual environments and their
applications -- Abstract -- 7.1 Introduction -- 7.2 EPS
from Deep Sea Hydrothermal Vents -- 7.3 EPS from Cold
Environments -- 7.4 EPS from Microbial Mats -- 7.5
Biomedial Applications of EPS -- 7.6 EPS as Antibiofilm
Agent -- 7.7 EPS as Biodetoxifiers -- 7.8 EPS and…..
Black Pearls -- 7.9 EPS in EOR/MEOR -- 7.10 EPS in
Cosmetics -- 7.11 Conclusions -- References -- Chapter 8:
Mechanical properties of Biofilms -- Abstract -- 8.1
Introduction -- 8.2 Mechanical Background -- 8.2.1 Hookean
solids and newtonian fluids -- 8.2.2 Non-linear behaviour
-- 8.2.3 Viscoelasticity -- 8.2.4 Rheology of viscoelastic
materials -- 8.2.4.1 Creep and relaxation tests -- 8.2.4.2
Dynamic test -- 8.2.4.3 Modelling viscoelasticity -- 8.3
Biofilm Mechanics -- 8.3.1 Macrorheological studies --
8.3.2 Microrehological studies -- 8.4 Biofilm Detachment -
- 8.5 Material Modelling of Biofilm Mechanics -- 8.6 ARE
Biofilm Mechanics and Function Correlated? --
Acknowledgments -- References
505 8 Chapter 9: Travelling through slime - bacterial movements
in the EPS matrix -- Abstract -- 9.1 Introduction:
Bacterial Movements Involved in the Biofilm Life-Cycle --
9.2 Existence of Flagella Propelled Motile Bacterial
Subpopulations in the EPS Matrix -- 9.3 Exploitation of
Bacterial Motility in Biofilm Control -- 9.3.1 Enhanced
biocide action -- 9.3.2 Delivery of antibacterials --
9.3.2.1 Targeting cell viability within biofilms --
9.3.2.2 Targeting matrix integrity within biofilms -- 9.4
Future Lines of Investigations -- Acknowledgements --
References -- Chapter 10: Why and how biofilms cause
biofouling - the "hair-in-sink"-effect -- Abstract -- 10.1
Introduction -- 10.2 Materials and Methods -- 10.2.1
Experimental set-up -- 10.2.2 Feed water -- 10.2.3 Optical
coherence tomography (OCT) -- 10.2.4 Biofilm thickness --
10.3 Results -- 10.3.1 Biofouling layer formation:
filtration or biofilm growth? -- 10.3.2 Impact of permeate
flux change on biofilm hydraulic resistance and thickness?
-- 10.3.3 Understanding the reason of hydraulic resistance
of biofilms: Model for the mechanism of water permeation -
- 10.4 Discussion -- Acknowledgements -- References --
Chapter 11: Unique and baffling aspects of the matrix: EPS
syneresis and glass formation during desiccation --
Abstract -- 11.1 Introduction -- 11.2 The Challenge of
Desiccation -- 11.2.1 Desiccation at the level of cells
and individual molecules -- 11.2.2 EPS and hydration-
maintenance -- 11.3 Microbial Mats: An EPS-Macrostructure
Having Microscale Architecture -- 11.3.1 Mat systems --
11.3.1.1 An EPS-based analog of Earth's earliest life --
11.3.2 Present-day mats -- 11.3.2.1 Anhydrophilic
hypersaline environments -- 11.3.2.2 Higher-salinity,
syneresis and development of EPS hydrophobic-skin --
11.3.2.3 Desiccation protection by EPS glass formation
505 8 11.3.2.4 Rehydration and rapid resumption of activities --
11.4 Conclusions -- Acknowledgements -- References --
Chapter 12: Extracellular factors involved in biofilm
matrix formation by Rhizobia -- Abstract -- 12.1 Overview
of Rhizobia and their Symbiotic Relation with Legumes --
12.2 Rhizobial Biofilm Formation -- 12.3 Rhizobial
Components of the Biofilm Matrix -- 12.3.1 Rhizobial
extracellular polysaccharides -- 12.3.1.1
Exopolysaccharides -- 12.3.1.2 Capsular polysaccharides --
12.3.1.3 Lipopolysaccharide -- 12.3.1.4 Glucomannan --
12.3.1.5 Cellulose -- 12.3.2 Extracellular proteins --
12.3.2.1 Adhesins -- 12.3.2.2 Lectins -- 12.3.2.3
Glycanases -- 12.3.3 Nod factors -- 12.3.4 Flagella --
12.4 Concluding Remarks -- Acknowledgements -- References
-- Chapter 13: Transparent exopolymeric particles: an
important EPS component in seawater -- Abstract -- 13.1
Introduction -- 13.2 Sources and Distributions of TEP in
the Ocean -- 13.2.1 Sources -- 13.2.2 Distribution in the
water column -- 13.2.3 Enrichment of TEP at the sea
surface -- 13.2.4 TEP in sea ice -- 13.3 Role of TEP in
the Marine Microbial Loop -- 13.3.1 TEP and bacteria --
13.3.2 TEP and microbial eukaryotes -- 13.3.3 TEP and
zooplankton -- 13.3.4 TEP and viruses -- 13.4 Conclusions
-- Acknowledgement -- References -- Chapter 14: Snapshots
of fungal extracellular matrices -- 14.1 Summary -- 14.2
The Diverse Matrix of Biofilms -- 14.3 Fungal Cell Wall
and Beyond -- 14.3.1 Polysaccharides -- 14.3.1.1 a-Glucans
-- 14.3.1.2 ß-glucans -- 14.3.1.3 Mannans: specific in
yeasts -- 14.4 Diverse Functions of Fungal EPS -- 14.4.1
Natural functions and their applications -- 14.4.2 EPS
support interactions with solid substrates including
minerals -- 14.4.3 Function of EPS in yeasts and melanised
yeast-like fungi -- 14.4.3.1 Capsule-forming fungi:
Cryptococcus species
505 8 14.5 Non-Polysaccharide Conpounds in the Extracellular
Matrix
588 Description based on publisher supplied metadata and other
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590 Electronic reproduction. Ann Arbor, Michigan : ProQuest
Ebook Central, 2020. Available via World Wide Web. Access
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650 0 Microbial polymers
655 4 Electronic books
700 1 Neu, Dr. Thomas R
700 1 Wingender, Dr Jost
776 08 |iPrint version:|aFlemming, Hans-Curt|tThe Perfect Slime :
Microbial Extracellular Polymeric Substances (EPS)|dLondon
: IWA Publishing,c2016|z9781780407418
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