LEADER 00000cam  2200673 i 4500 
001    864681043 
003    OCoLC 
005    20140317224531.0 
006    m     o  d         
007    cr cnu|unuuu|| 
008    131204s2014    nyua    ob    001 0 eng c 
020    9781627036702 (electronic bk.) 
020    1627036709 (electronic bk.) 
024 7  10.1007/978-1-62703-670-2 
035    (OCoLC)864681043 
040    GW5XE|beng|erda|epn|cGW5XE|dAS 
042    pcc 
050  4 QR414.5|b.H858 2014 
082 04 579.2/569|223 
245 00 Human retroviruses :|bmethods and protocols /|cedited by 
       Elisa Vicenzi and Guido Poli, Division of Immunology, 
       Transplant, and Infectious Diseases, San Raffaele 
       Scientific Institute, Milano, Italy 
264  1 New York :|bHumana Press,|c2014 
264  4 |c©2014 
300    1 online resource (xviii, 345 pages) :|billustrations 
       (some color) 
336    text|btxt|2rdacontent 
337    computer|bc|2rdamedia 
338    online resource|bcr|2rdacarrier 
490 1  Methods in Molecular Biology,|x1940-6029 ;|vVolume No. 
504    Includes bibliographical references and index 
505 00 |gPart I.|tMapping the HIV Life Cycle --|tVirion 
       Attachment and Entry: HIV gp120 Env Biotinylation, gp120 
       Env, or Integrin Ligand-Binding Assay  /|rClaudia Cicala 
       and James Arthos --|tCryoEM Analysis of Capsid Assembly 
       and Structural Changes Upon Interactions with a Host 
       Restriction Factor, TRIM5[alpha]  /|rGongpu Zhao and 
       Peijun Zhang --|tThe Fate of HIV-1 Capsid: A Biochemical 
       Assay for HIV-1 Uncoating /|rYang Yang, Jeremy Luban, and 
       Felipe Diaz-Griffero --|tThe Cyclosporin A Washout Assay 
       to Detect HIV-1 Uncoating  in  Infected Cells /|rAmy E. 
       Hulme and Thomas J. Hope --|tImaging HIV-1 Nuclear Pre-
       integration Complexes /|rAnna Cereseto and Mauro Giacca --
       |tHIV-1 Reverse Transcription /|rAndrea Cimarelli and Jean
       -Luc Darlix --|tRNase H: Specificity, Mechanisms of Action,
       and Antiviral Target  /|rKarin Moelling, Felix Broecker, 
       and John E. Kerrigan --|tHIV-1 Chromatin, Transcription, 
       and the Regulatory Protein Tat /|rLaurence Colin, Eric 
       Verdin, and Carine Van Lint --|tHIV-1 Rev Function and RNA
       Nuclear-Cytoplasmic Export /|rAlan Cochrane --|tHIV-1 
       Accessory Proteins: Nef /|rAnke Heigele, Daniel Sauter, 
       Jan Münch, and Frank Kirchhoff --|tHIV-1 Accessory 
       Proteins: VpR /|rRichard Y. Zhao and Michael I. Bukrinsky 
       --|tHIV-1 Accessory Proteins: Vpu and Vif /|rAmy Andrew 
       and Klaus Strebel --|tSIVSM/HIV-2 Vpx Proteins: Function 
       and Uses in the Infection of  Primary  Myeloid  Cells /
       |rGregory Berger and Andrea Cimarelli --|tImaging of HIV 
       Assembly and Release /|rBarbara Müller and Jacomine 
505 80 |gPart II.|tIsolation, Co-Receptor Use, and Cell Tropism 
       of HIV-1 --|tHIV-1 Isolation from Infected Peripheral 
       Blood Mononuclear Cells /|rStefania Dispinseri, Elisa Saba,
       Elisa Vicenzi, Neeltje A. Kootstra, Hanneke Schuitemaker, 
       and Gabriella Scarlatti --|tDetermination of HIV-1 Co-
       receptor Usage  /|rMariangela Cavarelli and Gabriella 
       Scarlatti --|tThe Macrophage and HIV: Basic Concepts and 
       Methodologies /|rSuzanne Gartner --|tHIV Infection of 
       Dendritic Cells /|rNajla Nasr, Andrew Harman, Stuart 
       Turville, and Anthony L. Cunningham --|tHistocultures 
       (Tissue Explants) in Human Retrovirology /|rAnush 
       Arakelyan, Wendy Fitzgerald, Jean-Charles Grivel, 
       Christophe Vanpouille, and Leonid Margolis 
505 80 |gPart III.|tIn Vivo Quantification of HIV-1 --|tSingle-
       Copy Quantification of HIV-1 in Clinical Samples /|rAnn 
       Wiegand and Frank Maldarelli --|tQuantification of Total 
       HIV1-DNA in Peripheral Blood Mononuclear Cells /
       |rChristine Rouzioux, Adeline Mélard, and Véronique 
505 80 |gPart IV.|tBiotechnological Aspects of HIV-1 --|tHIV-1-
       Based Lentiviral Vectors /|rYing Poi Liu and Ben Berkhout 
       --|tQuantification of miRNA by Poly(A)-RT-qPCR Arrays and 
       Verification of Target Sites in HIV-1 Using a One-LTR 
       Infectious Molecular Clone /|rZachary A. Klase, Laurent 
       Houzet, and Kuan-Teh Jeang 
505 80 |gPart V.|tHTLVS --|tInvestigating Human T Cell 
       Lymphotropic Retrovirus (HTLV) Tax Function with Molecular
       and Immunophenotypic Techniques /|rGreta Forlani, Roberto 
       S. Accolla, and Giovanna Tosi --|tProviral Load 
       Determination of HTLV-1 and HTLV-2 in Patients' Peripheral
       Blood Mononuclear Cells by Real-Time PCR /|rClaudio Casoli,
       Elisabetta Pilotti, and Umberto Bertazzoni --
       |tQuantitative Analysis of Human T-Lymphotropic Virus Type
       1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic 
       Fractionation and Splice Junction-Specific Real-Time RT-
       PCR (qRT-PCR) /|rIlaria Cavallari, Francesca Rende, and 
       Vincenzo Ciminale 
520    "Human Retroviruses: Methods and Protocols collects key 
       experimental protocols that have provided the basis of the
       major discoveries of the field. Split into five sections, 
       this detailed volume covers mapping of the HIV life cycle,
       isolation, co-receptor use, and cell tropism of HIV-1, in 
       vivo quantification of HIV-1, biological aspects of HIV-1,
       as well as HTLVs. Some articles explore assay and function
       of accessory genes, largely involving the interface 
       between retroviral and host factors, the extracellular 
       role of Tat and Tax, resembling the function of cytokines,
       and the biotechnological exploitation of HIV as lentiviral
       vector to carry foreign genes with therapeutic value. 
       Written in the highly successful Methods in Molecular 
       Biology series format, chapters include introductions to 
       their respective topics, lists of the necessary materials 
       and reagents, step-by-step, readily reproducible 
       laboratory protocols, and tips on troubleshooting and 
       avoiding known pitfalls. Comprehensive and authoritative, 
       Human Retroviruses: Methods and Protocols provides state-
       of-art methodological protocols from world leaders in 
       human retrovirology, essential for any lab working this 
       vital field."--Publisher's description 
588    Description based on online resource; title from PDF title
       page (Springer Protocols, viewed January 30, 2014) 
650  0 Retroviruses|vLaboratory manuals 
650  0 HIV (Viruses)|vLaboratory manuals 
650  0 HTLV (Viruses)|vLaboratory manuals 
650 12 Retroviridae|vLaboratory Manuals 
650 12 HIV|vLaboratory Manuals 
650 12 Deltaretrovirus|vLaboratory Manuals 
650 14 Biomedicine 
650 24 Immunology 
650 24 Virology 
650 24 Laboratory Medicine 
700 1  Vicenzi, Elisa,|eeditor of compilation.|4edc 
700 1  Poli, Guido,|eeditor of compilation.|4edc 
776 08 |iPrint version:|tHuman retroviruses.|dNew York : Humana 
       Press, 2014|z1627036695|z9781627036696|w(DLC)  2013949423
830  0 Methods in molecular biology ;|v1087 
856 40 |3SpringerLink|uhttp://dx.doi.org/10.1007/978-1-62703-670-
       2|zeBook(Springerlink)(Life Science Group only)