MARC 主機 00000nam  2200349   4500 
001    AAI1449181 
005    20081121150438.5 
008    081121s2007    ||||||||||||||||| ||eng d 
020    9780549286547 
035    (UMI)AAI1449181 
040    UMI|cUMI 
100 1  Devanney, Sean C 
245 10 Study of the coupling between transcription and mRNA 
       processing utilizing a novel Bcl-x mini-gene 
300    55 p 
500    Source: Masters Abstracts International, Volume: 46-02, 
       page: 0851 
500    Adviser: Massimo Caputi 
502    Thesis (M.S.)--Florida Atlantic University, 2007 
520    The Bcl family of genes are fundamental to the apoptotic 
       mechanism. Bcl-x a member of this family, is alternatively
       spliced to create two main isoforms a long (Bcl-xL) and a 
       short (Bcl-xS) variant. The long form exhibits anti-
       apoptotic activity, while the short form favors apoptosis.
       The proper balance of expression of these two isoforms is 
       crucial for several developmental processes such as thymic
       selection and neural reshaping. A number of cancer types 
       have been shown to over-express the long form, thereby 
       granting them some protection from apoptosis. To study the
       transcriptional and post-transcriptional mechanisms 
       regulating gene expression, the Bcl-x gene has been 
       utilized. A complex mini-gene construct has been create in
       order to monitor the effects that promoter sequences, 
       5'UTR and 3'UTR's have on mRNA splicing, RNA export, 
       stability and translation. Abundant evidence exists 
       indicating that RNA processing events such as 
       transcription, splicing and export are coupled, yet the 
       mechanisms and factors involved in regulating these 
       processes are poorly understood. The mini-gene is 
       identical to the endogenous gene with the exception of a 
       deletion to the 50Kb intron and the addition of a tag to 
       differentiate the mini-gene product from the endogenous 
       mRNA and protein. This novel system allows for the study 
       of transcriptional and post-transcriptional mechanisms 
       regulating gene expression from RNA biogenesis on to the 
       protein level 
590    School code: 0119 
590    DDC 
650  4 Biology, Molecular 
650  4 Biology, Genetics 
650  4 Biology, Cell 
690    0307 
690    0369 
690    0379 
710 2  Florida Atlantic University 
773 0  |tMasters Abstracts International|g46-02 
856 40 |u