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書名 CRISPR : methods and protocols / edited by Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran
出版項 New York, NY : Humana Press, 2015
國際標準書號 9781493926879
國際標準號碼 10.1007/978-1-4939-2687-9
book jacket
說明 1 online resource (xiv, 366 pages) : illustrations (some color)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
系列 Methods in Molecular Biology, 1940-6029 ; volume 1311
Methods in molecular biology ; 1311
附註 Includes bibliographical references and index
Online resource; title from PDF title page (SpringerLink, viewed May 22, 2015)
Investigating CRISPR RNA Biogenesis and Function using RNA-seq -- In vitro Co-reconstitution of Cas Protein Complexes -- Analysis of CRISPR pre-crRNA Cleavage -- Annotation and Classification of CRISPR-Cas Systems -- Computational Detection of CRISPR/crRNA Targets -- High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium -- Spacer-based Macroarrays for CRISPR Genotyping -- Analysis of crRNA using Liquid Chromatography Electrospray Ionisation Mass Spectrometry (LC ESI MS) -- Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection through CRISPR -- Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification Of Target Interference Motifs in Escherichia coli -- Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes -- Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus -- Procedures for Generating CRISPR Mutants With Novel Spacers Acquired from Viruses Or Plasmids -- Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition -- Using the CRISPR-Cas system to Positively Select Mutants In Genes Essential for its Functionl -- Analysis of Nuclease Activity of Cas1 Proteins Against Complex DNA Substrates -- Characterizing Metal-Dependent Nucleases of CRISPR-Cas prokaryotic Adaptive Immunity Systems -- Cas3 Nuclease-Helicase Activity Assays -- Chemical and Enzymatic Footprint Analyses of R-loop Formation by Cascade-crRNA Complex -- Creation and Analysis of a Virome: Using CRISPR Spacers -- Targeted Mutagenesis in Zebrafish using CRISPR RNA-guided Nucleases -- Precise Genome Editing of Drosophila with CRISPR RNA-guided Cas9 -- Targeted Transcriptional Repression in Bacteria using CRISPR Interference (CRISPRi)
This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. OCLCbrA0 CRISPR: Methods and Protocols OCLCbrA0 guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from OCLCbrA0 standard protocols of DNA, RNA and protein biology. OCLCbrA0 Written in the highly successful OCLCbrA0 Methods in Molecular Biology OCLCbrA0 series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. OCLCbrA0 Authoritative and cutting-edge, OCLCbrA0 CRISPR: Methods and Protocols OCLCbrA0 provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems
Includes bibliographical references and index (pages 362-366)
鏈接 Print version: CRISPR. New York, NY : Humana Press, 2015 1493926861 9781493926862 (DLC) 2015937760
主題 CRISPR (Genetics)
Genetic regulation
Bacterial genetics
CRISPR-Associated Proteins -- analysis
Electronic books
Laboratory Manuals
Alt Author Lundgren, Magnus, editor
Charpentier, Emmanuelle, editor
Fineran, Peter C., editor
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