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書名 Nuclear bodies and noncoding RNAs : methods and protocols / edited by Shinichi Nakagawa, Tetsuro Hirose
出版項 New York, NY : Humana Press, 2015
國際標準書號 9781493922536
149392253X
國際標準號碼 10.1007/978-1-4939-2253-6
book jacket
說明 1 online resource (xiiI, 351 pages) : illustrations (some color)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
系列 Methods in Molecular Biology, 1064-3745 ; 1262
Methods in molecular biology ; 1262
附註 Includes index
Visualization of lncRNA by single-molecule fluorescence in situ hybridization / Margaret Dunagin , Moran N. Cabili , John Rinn , and Arjun Raj -- Super-resolution imaging of nuclear bodies by STED microscopy / Yasushi Okada and Shinichi Nakagawa -- High-resolution 3D DNA FISH using plasmid probes and computational correction of optical aberrations to study chromatin structure at the sub-megabase scale / Luca Giorgetti, Tristan Piolot, and Edith Heard -- Time-lapse imaging of nuclear bodies / Saskia Hutten, Samuel Swift, and Angus I. Lamond -- Visualization of nucleic acids with synthetic exciton- controlled fluorescent oligonucleotide probes / Dan Ohtan Wang and Akimitsu Okamoto -- Live CLEM imaging to analyze nuclear structures at high resolution / Tokuko Haraguchi, Hiroko Osakada, and Takako Koujin -- Ultrastructural analysis of nuclear bodies using electron microscopy / Sylvie Souquere and Gérard Pierron -- Analyses of nuclear proteins and nucleic acid structures using atomic force microscopy / Jamie L. Gilmore ... [et al.] -- Genome-wide co-localization screening of nuclear body components using a fluorescently tagged FLJ cDNA clone library / Tetsuro Hirose and Naoki Goshima -- Purification of specific chromatin regions using oligonucleotides : capture hybridization analysis of RNA targets (CHART) / Christopher P. Davis and Jason A. West -- RNA antisense purification (RAP) for mapping RNA interactions with chromatin / Jesse Engreitz, Eric S. Lander, and Mitchell Guttman -- In situ dissection of RNA functional subunits by domain- specific chromatin isolation by RNA purification (dChIRP) / Jeffrey J. Quinn and Howard Y. Chang -- Extracting, enriching, and identifying nuclear body sub-complexes using label-based quantitative mass spectrometry / Archa Fox ... [et al.] -- Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells : native ribonucleoprotein immunoprecipitation (RIP) assays / Joseph A. Cozzitorto ... [et al.] -- Cross-linking and immunoprecipitation of nuclear RNA- binding proteins / Quan Li , Yuri Uemura , and Yukio Kawahara -- Purification of noncoding RNA and bound proteins using FLAG peptide-conjugated antisense-oligonucleotides / Shungo Adachi and Tohru Natsume -- MMCT-mediated chromosome engineering technique applicable to functional analysis of lncRNA and nuclear dynamics / Makiko Meguro-Horike and Shin-ichi Horike -- Reconstitution of nucleocytoplasmic transport using digitonin-permeabilized cells / Shingo Kose, Tomoko Funakoshi, and Naoko Imamoto -- Genome-wide analysis of long noncoding RNA turnover / Hidenori Tani ... [et al.] -- Knockdown of nuclear-retained long noncoding RNAs using modified DNA antisense oligonucleotides / Xinying Zong ... [et al.] -- siRNA screening of nuclear proteins / Yuko Hasegawa and Shinichi Nakagawa
Online resource; title from PDF title page (SpringerLink, viewed January 23, 2015)
鏈接 Print version: Nuclear bodies and noncoding RNAs : methods and protocols 9781493922529
主題 Non-coding RNA -- Laboratory manuals
Nuclear proteins -- Laboratory manuals
Biomedicine
Human Genetics
Electronic books
Alt Author Nakagawa, Shinichi, editor
Hirose, Tetsuro, editor
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