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書名 Proteomics for drug discovery [electronic resource] : methods and protocols / edited by Iulia M. Lazar, Maria Kontoyianni, Alexandru C. Lazar
出版項 New York, NY : Springer New York : Imprint: Humana Press, 2017
國際標準書號 9781493972012
國際標準號碼 10.1007/978-1-4939-7201-2 doi
book jacket
說明 1 online resource (xi, 302 pages) : illustrations
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
text file PDF rda
系列 Methods in molecular biology ; 1647
Methods in molecular biology ; 1647
附註 A Photoaffinity Labeling-Based Chemoproteomics Strategy for Unbiased Target Deconvolution of Small Molecule Drug Candidates -- Multiplexed Liquid Chromatography-Multiple Reaction Monitoring Mass Spectrometry Quantification of Cancer Signaling Proteins -- Monitoring Dynamic Changes of the Cell Surface Glycoproteome by Quantitative Proteomics -- High Resolution Parallel Reaction Monitoring with Electron Transfer Dissociation for Middle-Down Proteomics: An Application to Study the Quantitative Changes Induced by Histone Modifying Enzyme Inhibitors and Activators -- Preparation and Immunoaffinity Depletion of Fresh Frozen Tissue Homogenates for Mass Spectrometry-Based Proteomics in the Context of Drug Target/Biomarker Discovery -- Target Identification Using Cell Permeable and Cleavable Chloroalkane Derivatized Small Molecules -- Microfluidics-Mass Spectrometry of Protein-Carbohydrate Interactions: Applications to Development of Therapeutics and Biomarker Discovery -- Studying Protein-Protein Interactions by Biotin AP-Tagged Pulldown and LTQ-Orbitrap Mass Spectrometry -- Post-Translational Modification Profiling-Functional Proteomics for the Analysis of Immune Regulation -- Reverse Phase Protein Arrays and Drug Discovery -- Probing Protein Kinase-ATP Interactions Using a Fluorescent ATP Analog -- Preparation of Disease-Related Protein Assemblies for Single Particle Electron Microscopy -- Identification of Lipid-Binding Modulators Using the Protein-Lipid Overlay Assay -- Resazurin Live Cell Assay: Setup and Fine-Tuning for Reliable Cytotoxicity -- Exploring Protein-Protein Interactions as Drug Targets for Anti-Cancer Therapy with In Silico Workflows -- Method to Identify Silent Codon Mutations that May Alter Peptide Elongation Kinetics and Co-Translational Protein Folding -- In Silico Design of Anticancer Peptides -- Docking and Virtual Screening in Drug Discovery -- Bioinformatics Resources for Interpreting Proteomics Mass Spectrometry Data
This book reflects the drug development community’s interest in proteomics methods by providing researchers with the necessary knowledge for the implementation of a number of basic experimental and bioinformatics protocols in the drug discovery research laboratory. The focus of the collection is on select protocols and methodologies that address the analysis of post-translational modifications, targeted protein quantification, protein-protein, protein-lipid, or protein-ATP interactions, in silico lead identification, protein arrays, tissue and cell extract preparation, labeling, chemoproteomics, and drug efficacy assessment. Emphasis was placed not just on methods that describe large-scale protein analysis, but also on ones that are amenable to future implementation in a high-throughput format. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Practical and authoritative, Proteomics for Drug Discovery: Methods and Protocols aims to provide a deep knowledge of the fundamentals of disease biology as well the means that were developed for probing the complex biological milieu
Host Item Springer eBooks
鏈接 Printed edition: 9781493972005
主題 Medicine
Alt Author Lazar, Iulia M. editor
Kontoyianni, Maria. editor
Lazar, Alexandru C. editor
SpringerLink (Online service)
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