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作者 Lee, Alexander Seogwon
書名 Atomic force microscopy applications in sensing and actuation
國際標準書號 9780542912849
book jacket
說明 139 p
附註 Source: Dissertation Abstracts International, Volume: 67-10, Section: B, page: 5899
Advisers: Theodore T. Tsotsis; Aristides A. G. Requicha
Thesis (Ph.D.)--University of Southern California, 2006
Atomic Force Microscopy (AFM) is applied in two areas of nanotechnology: (1) developing nanobiosensors for cell detection, and (2) testing the actuation response of nanoactuators
Aureococcus anophagefferens (A. anophagefferens) is studied by AFM for the first time, and is used as the target for cell identification amongst other similarly sized and shaped cells. A microcantilever-based biosensor is made by functionalizing an AFM probe with a monoclonal antibody (MAb) specific to A. anophagefferens. Force spectroscopy of the rupture forces between the MAb on the probe and a cell is the basis for sensing. The interaction force between a single MAb and an A. anophagefferens cell is found to be 252 +/-24 pN and 277 +/-17 pN for loadrates of 45 nN/s and 60 nN/s, respectively. This is in clear contrast to the rupture forces found between the MAb and other cells, which mostly have values of less than 100 pN, and are attributed to non-specific binding. A biosensor having high-signal-to-noise ratio is based on the percentage of rupture force events attributed to specific recognition. The advantage of this technique is its potential for miniaturization and multiplexing as well as a reduction of time in testing single cells with high degree of confidence
Artificial nanorobots do not yet exist, and there are many important technological challenges that need to be addressed for building them. Enabling the movement of these tiny robots is one such challenge. Polypyrrole (PPy) is an electroactive polymer which can change volume based on its oxidation state. PPybased macro and microscale actuators have been demonstrated, but their nanoscale counterparts have yet to be realized. PPy nanowires are studied in this thesis for their potential use as nanoactuators. These nanowires, 50 nm and 100 nm in diameter and approximately 6 microns in length, are fabricated by chemical polymerization using track-etched polycarbonate membranes as templates. Their actuation response as a function of oxidation state is investigated by electrochemical AFM. An estimate of the minimum actuation force was made based on the displacement of the AFM cantilever. This research shows for the first time that PPy nanowires exhibit actuation behavior
School code: 0208
Host Item Dissertation Abstracts International 67-10B
主題 Chemistry, Polymer
Engineering, Chemical
Alt Author University of Southern California
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