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作者 Maxson, Scott Anthony
書名 The development of a mesenchymal stem cell based biphasic osteochondral tissue engineered construct
國際標準書號 9781124651033
book jacket
說明 277 p
附註 Source: Dissertation Abstracts International, Volume: 72-08, Section: B, page: 4794
Adviser: Karen J.L. Burg
Thesis (Ph.D.)--Clemson University, 2010
The ability of human articular cartilage to respond to injury is poor. Once cartilage damage has occurred, an irreversible degenerative process can occur and will often lead to osteoarthritis (OA). An estimated 26.9 million of U.S. adults are affected by OA. Osteochondral grafting is currently used to treat OA and osteochondral defects; however, complications can develop at the donor site and defect area. Osteochondral tissue engineering provides a potential treatment option and alternative to osteochondral grafting. The long term goal of this work is to develop a tissue engineered mesenchymal stem cell (MSC) based osteochondral construct to repair cartilage damage
The first set of studies evaluated the interactions between skeletal tissue cells and the effectiveness of conditioned media (CM) to modulate mesenchymal stem cell differentiation. The results showed that CM can enhance the differentiation of MSCs towards several different cell types depending on the type of CM
The next set of studies involved the design and characterization of a novel biphasic osteochondral tissue engineered construct. The osteochondral constructs were designed to address some of the current limitations that osteochondral tissue engineering currently faces; i.e., providing an alternate method of anchoring the construct and providing the ability to generate large viable constructs. Based on the results of several studies, a composite of polylactide/polycaprolactone and hydroxyapatite was used for the bone phase, a barbed pin was used to create a stable implant, and agarose was chosen for use in the cartilage phase. In addition, capillary channel fibers were incorporated in the bone phase to provide nutrient and cell delivery. The bundles of capillary channel fibers embedded in large tissue engineering scaffolds were shown to facilitate a uniform cell distribution through large volume scaffolds
In the next study, a modified bioreactor was designed and incorporated a pneumatically controlled syringe to generate hydrostatic pressure (HP) on both phases of the biphasic construct. The bioreactor was used to demonstrate synergistic effects of CM and HP on the chondrogenic and osteogenic differentiation of MSCs
Ultimately, in a final study, the combination of CM, bioreactor cultivation, and the unique construct design generated a biphasic construct with a cartilage-like upper phase attached to a bone-like bottom phase
School code: 0050
Host Item Dissertation Abstracts International 72-08B
主題 Engineering, Biomedical
Alt Author Clemson University. Bioengineering
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