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書名 Reverse phase protein arrays : from technical and analytical fundamentals to applications / edited by Tesshi Yamada, Satoshi S. Nishizuka, Gordon B. Mills, Lance A. Liotta
出版項 Singapore : Springer Singapore : Imprint: Springer, 2019
國際標準書號 9789813297555 (electronic bk.)
9789813297548 (paper)
國際標準號碼 10.1007/978-981-32-9755-5 doi
book jacket
說明 1 online resource (viii, 266 pages) : illustrations, digital ; 24 cm
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
text file PDF rda
系列 Advances in experimental medicine and biology, 0065-2598 ; volume 1188
Advances in experimental medicine and biology ; volume 1188
附註 Chapter 1: Origin and future of RPPA technology -- Chapter 2: Lysate preparation -- Chapter 3: Printing platforms 1 -- Chapter 4: Printing platforms 2 -- Chapter 5: Analytical platforms 1 -- Chapter 6: Analytical platforms 2 -- Chapter 7: Analytical platforms 3 -- Chapter 8. Antibody screening 1 -- Chapter 9. Antibody screening 2 -- Chapter 10. Antibody screening 3 -- Chapter 11. Integrative analysis for genomics and RPPA -- Chapter 12. Integrative analysis of multiple platforms of RPPA -- Chapter 13. Drug screening platforms and RPPA -- Chapter 14. RPPA in small population analysis -- Chapter 15: Molecular target identification using RPPA -- Chapter 16: RPPA in clinical trials -- Chapter 17: RPPA and future medicine
This book provides practical guidance on all aspects of reverse phase protein array (RPPA) technology, which permits the quantification of protein levels in cell or tissue lysates. In addition, the latest results are presented from laboratories across the world where experts are successfully running the challenging RPPA data platform. After an introductory chapter by the inventor of the RPPA technique, the demanding task of lysate preparation is explained. Subsequent chapters review printing platforms and analytical platforms and discuss antibody screening in detail. The role of RPPA data in integrative analyses is thoroughly examined, and the various applications of RPPA, for example to identify molecular targeting drugs or evaluate cancer drug efficacy, are discussed with reference to the latest knowledge. It is hoped that the book will foster the implementation of RPPA by documenting the practical details of the technology and by revealing its great potential. It will be of high value for researchers who plan to build an RPPA platform in their institute and for researchers, clinicians, and companies who use RPPA through collaboration with an existing RPPA facility
Host Item Springer eBooks
主題 Proteins
Proteins -- Research
Molecular Medicine
Cancer Research
Protein Science
Biomedical Engineering/Biotechnology
Microarrays
Pharmaceutical Sciences/Technology
Alt Author Yamada, Tesshi, editor
Nishizuka, Satoshi S., editor
Mills, Gordon B., editor
Liotta, Lance A., editor
SpringerLink (Online service)
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