MARC 主機 00000nam  2200337   4500 
001    AAI3145564 
005    20051017073533.5 
008    051017s2004                        eng d 
020    0496045261 
035    (UnM)AAI3145564 
040    UnM|cUnM 
100 1  Munro, Jeffrey Christian 
245 10 In situ formation and characterization of polymer-
       supported lipid bilayers 
300    252 p 
500    Source: Dissertation Abstracts International, Volume: 65-
       09, Section: B, page: 4710 
500    Adviser:  Curtis W. Frank 
502    Thesis (Ph.D.)--Stanford University, 2004 
520    Tethered lipid bilayer membranes provide a model for 
       cellular systems and have potential applications in areas 
       such as biosensors. Inclusion of a polymer cushion between
       a lipid bilayer membrane and solid surface has been 
       suggested as a means to provide a soft, deformable layer 
       that will allow for transmembrane protein insertion and 
       mobility. In this work, we present methods for the self-
       assembly of multifunctional polymers that provide a 
       substantial hydrophilic cushion to create tethered lipid 
       bilayers in situ 
520    First, in order to better understand the properties of the
       polymer cushion, an extensive study of the viscoelastic 
       properties of thin polyacrylamide (PA) films was performed
       with a quartz crystal microbalance with dissipation (QCM-
       D). Using a continuum mechanics viscoelastic film model, 
       we extract film property parameters from frequency and 
       dissipation change measurements for multiple harmonics 
520    Second, we have studied the adsorption behavior and 
       viscoelastic properties of two lipopolymers: a PA-based 
       random copolymer and an end-functionalized poly (ethylene 
       glycol) (PEG). Both lipopolymers are functionalized with 
       lipids for anchoring into a bilayer and disulfides for 
       covalent attachment to gold or silver surfaces. The 
       adsorption of both lipopolymers to gold surfaces has been 
       studied using a variety of surface-sensitive 
       characterization techniques. Our experiments indicate that
       the PEG lipopolymer has several favorable characteristics 
       compared to the PA-based lipopolymer, including thicker 
       polymer cushions, more complete chemisorption to the 
       surface, and better control over the tethered lipid 
       density. Additional experiments indicate that the position
       of the lipid end groups can be controlled by adsorbing the
       PEG lipopolymer from various solvents. Finally, the 
       surface density of tethered lipid groups can be controlled
       by co-adsorbing the PEG lipopolymer with an analogous 
       methoxy-terminated PEG molecule 
520    Finally, mobile, tethered lipid bilayers were formed on 
       the adsorbed PEG films via a two-step adsorption process. 
       First, lipids were adsorbed onto the PEG support from a 
       hexane solution. Second, vesicles were adsorbed and fused 
       on the surface to create a bilayer in an aqueous 
       environment. Fluorescence recovery after photobleaching 
       experiments show that this process results in mobile 
       bilayers with diffusion constants on the order of 2 mum 2/
       s 
590    School code: 0212 
590    DDC 
650  4 Engineering, Chemical 
690    0542 
710 20 Stanford University 
773 0  |tDissertation Abstracts International|g65-09B 
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