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作者 Munro, Jeffrey Christian
書名 In situ formation and characterization of polymer-supported lipid bilayers
國際標準書號 0496045261
book jacket
說明 252 p
附註 Source: Dissertation Abstracts International, Volume: 65-09, Section: B, page: 4710
Adviser: Curtis W. Frank
Thesis (Ph.D.)--Stanford University, 2004
Tethered lipid bilayer membranes provide a model for cellular systems and have potential applications in areas such as biosensors. Inclusion of a polymer cushion between a lipid bilayer membrane and solid surface has been suggested as a means to provide a soft, deformable layer that will allow for transmembrane protein insertion and mobility. In this work, we present methods for the self-assembly of multifunctional polymers that provide a substantial hydrophilic cushion to create tethered lipid bilayers in situ
First, in order to better understand the properties of the polymer cushion, an extensive study of the viscoelastic properties of thin polyacrylamide (PA) films was performed with a quartz crystal microbalance with dissipation (QCM-D). Using a continuum mechanics viscoelastic film model, we extract film property parameters from frequency and dissipation change measurements for multiple harmonics
Second, we have studied the adsorption behavior and viscoelastic properties of two lipopolymers: a PA-based random copolymer and an end-functionalized poly (ethylene glycol) (PEG). Both lipopolymers are functionalized with lipids for anchoring into a bilayer and disulfides for covalent attachment to gold or silver surfaces. The adsorption of both lipopolymers to gold surfaces has been studied using a variety of surface-sensitive characterization techniques. Our experiments indicate that the PEG lipopolymer has several favorable characteristics compared to the PA-based lipopolymer, including thicker polymer cushions, more complete chemisorption to the surface, and better control over the tethered lipid density. Additional experiments indicate that the position of the lipid end groups can be controlled by adsorbing the PEG lipopolymer from various solvents. Finally, the surface density of tethered lipid groups can be controlled by co-adsorbing the PEG lipopolymer with an analogous methoxy-terminated PEG molecule
Finally, mobile, tethered lipid bilayers were formed on the adsorbed PEG films via a two-step adsorption process. First, lipids were adsorbed onto the PEG support from a hexane solution. Second, vesicles were adsorbed and fused on the surface to create a bilayer in an aqueous environment. Fluorescence recovery after photobleaching experiments show that this process results in mobile bilayers with diffusion constants on the order of 2 mum 2/s
School code: 0212
Host Item Dissertation Abstracts International 65-09B
主題 Engineering, Chemical
Alt Author Stanford University
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