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作者 Soto-Gutierrez, Alejandro
書名 Methods in Bioengineering : Cell Transplantation
出版項 Norwood : Artech House, 2011
©2011
國際標準書號 9781608070169 (electronic bk.)
9781608070152
book jacket
版本 1st ed
說明 1 online resource (272 pages)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
附註 Methods in Bioengineering: Cell Transplantation -- Contents -- Preface -- 1 Extracellular Matrices for Hepatic Tissue Engineering -- 1.1 Introduction -- 1.2 Experimental Design -- 1.3 Materials -- 1.4 Methods -- 1.4.1 Cell Adhesion Assay -- 1.4.2 Cell Detachment Assay -- 1.4.3 Inhibition Assay -- 1.4.4 Western Blot Assay -- 1.4.5 Flow Cytometry Analysis -- 1.4.6 Lectin Binding Assay -- 1.4.7 Coupling of Galactose Groups with ECM Bulk and ECM Surface -- 1.4.8 Regulation of Density of Galactose and Spatial Orientation ofGalactose -- 1.4.9 Regulation of Topology of ECM -- 1.4.10 Regulation of Deposition of Natural ECM -- 1.4.11 Design of Coculture System -- 1.4.12 Choice of Cell Sources -- 1.5 Anticipated Results -- 1.6 Discussion and Commentary -- 1.7 Application Notes -- 1.8 Summary Points -- Acknowledgments -- References -- 2 Tissue Engineering Using a Temperature-Responsive Cell Culture Dish -- 2.1 Introduction -- 2.2 Experimental Design -- 2.3 Materials -- 2.4 Methods -- 2.4.1 Seeding Cells -- 2.4.2 Change Medium -- 2.4.3 Harvest of Cell Sheets -- 2.4.4 Transplantation of Cell Sheets -- 2.4.5 Multilayering of Cell Sheets -- 2.5 Data Acquisition, Anticipated Results, andInterpretation -- 2.5.1 Autograft/Allograft Models -- 2.5.2 Xenograft Models -- 2.6 Discussion and Commentary -- 2.7 Applications Notes -- 2.7.1 Single Cells -- 2.7.2 Cell Sheets -- 2.8 Summary Points -- Acknowledgments -- References -- 3 In-Vivo Functional Assessment of Engineered Human Insulin-Producing Cells -- 3.1 Introduction -- 3.2 Experimental Design -- 3.3 Materials -- 3.4 Methods -- 3.4.1 Generation of Hyperglycemic Immunodeficient Recipient Mice -- 3.4.2 Cell Transplantation Under the Renal Capsule -- 3.4.3 Monitoring Blood Glucose, Insulin, and C-Peptide Levels -- 3.4.4 Assessment of Glucose Responsiveness Using GTT
3.4.5 Survival Left-Side Nephrectomy and Tissue Removal -- 3.4.6 Graft Histological and RNA Analyses -- 3.5 Anticipated Results -- 3.6 Discussion and Commentary -- 3.7 Application Notes -- 3.8 Summary Points -- References -- 4 Generation of Insulin-Producing Cells from Adult Mouse Intrahepatic Biliary Epithelial Cells In Vitro -- 4.1 Introduction -- 4.2 Experimental Design -- 4.2.1 Generation of Insulin-Producing Cells from IHBECs -- 4.3 Materials -- 4.3.1 Reagents and Equipment -- 4.3.2 Equipment -- 4.3.3 Reagent Setup -- 4.3.4 Adenovirus Transduction -- 4.3.5 Reverse Transcription Polymerase Chain Reaction (RT-PCR) -- 4.3.6 Immunocytochemistry -- 4.3.7 Measurement of Insulin Production -- 4.3.8 Analysis of Single Cells by RT-PCR -- 4.4 Methods -- 4.4.1 Step 1 -- 4.4.2 Mouse Biliary Epithelial Cell Isolation and Culture -- 4.4.3 Step 2 -- 4.4.4 Step 3 -- 4.4.5 Step 4 -- 4.4.6 Step 5 -- 4.4.7 Evaluation -- 4.4.8 Statistical Analysis -- 4.5 Data Acquisition, Anticipated Results, and Interpretation -- 4.6 Discussion and Commentary -- 4.7 Summary Points -- Acknowledgments -- References -- 5 Technologies to Improve Human Islet Transplantation -- 5.1 Introduction -- 5.2 Materials -- 5.2.1 Pancreas Procurement and Preservation -- 5.2.2 Islet Isolation -- 5.2.3 Islet Culture/Preservation (Optional) -- 5.2.4 Islet Transplantation -- 5.3 Methods -- 5.3.1 Pancreas Procurement for Islet Isolation -- 5.3.2 Pancreas Procurement from Heart-Beating Brain-Dead Donors -- 5.3.3 Pancreas Procurement from Non-Heart-Beating Donors -- 5.3.4 Pancreas Preservation -- 5.3.5 Islet Isolation -- 5.3.6 Islet Culture/Preservation (Optional) -- 5.3.7 Islet Transplantation -- 5.3.8 Potential Pitfalls and Troubleshooting -- 5.4 Data Acquisition, Anticipated Results, and Interpretation -- 5.4.1 Islet Isolations from Brain-Dead Donors
5.4.2 Islet Isolations from Non-Heart-Beating Donors -- 5.4.3 Islet Transplantation from Living Donors -- 5.5 Discussion and Commentary -- 5.6 Summary Points -- Acknowledgments -- References -- 6 Pancreatic Islet Isolation from Swine -- 6.1 Introduction -- 6.2 Materials -- 6.2.1 Animals -- 6.2.2 Younger Versus Older Pigs -- 6.3 Equipment -- 6.3.1 Major Equipment -- 6.3.2 Small Equipment and Nondisposable Items -- 6.3.3 Disposable Items -- 6.4 Media -- 6.5 Methods -- 6.5.1 Harvesting the Pancreas -- 6.5.2 Laboratory Setup -- 6.5.3 Cleaning and Cannulating Pancreas -- 6.5.4 Enzyme Preparation -- 6.5.5 Polysucrose Purification of Tissue -- 6.5.6 Counting Islet Equivalents -- 6.5.7 Culture -- 6.5.8 Assessment of Islet Quality -- 6.6 Anticipated Results -- 6.7 Discussion and Commentary -- 6.8 Summary -- Acknowledgments -- References -- 7 Spinal Cord Injury Models and Cell Transplantation Therapy -- 7.1 Introduction -- 7.2 Experimental Design -- 7.2.1 Pathophysiologic States of Spinal Cord Injury and Therapeutic Strategies -- 7.2.2 Induction of Models Suitable for the Study Objectives -- 7.2.3 Controls Required for the Experiments -- 7.3 Materials and Methods -- 7.3.1 Spinal Cord Section Model -- 7.3.2 Spinal Contusion Injury Model -- 7.3.3 Demyelination Model -- 7.3.4 Cell Transplantation -- 7.4 Data Acquisition, Anticipated Results, andInterpretation -- 7.4.1 Function Assessment Methods -- 7.5 Discussion and Commentary -- 7.5.1 Ethical Aspects -- 7.6 Application Notes -- 7.7 Summary Points -- References -- 8 Cell Transplantation and Regeneration in Neurology -- 8.1 Introduction -- 8.2 Experimental Design -- 8.3 Materials -- 8.3.1 Preparation for Cell Suspension -- 8.3.2 Stereotaxic Intracerebral Cell Transplantation (Rat) -- 8.3.3 Encapsulated Cell Production -- 8.4 Methods -- 8.4.1 Preparation of Cell Suspension
8.4.2 Stereotaxic Intracerebral Cell Transplantation (Rat) -- 8.4.3 Making of Encapsulated Cells -- 8.5 Data Acquisition, Anticipated Results, and Interpertation -- 8.6 Discussion and Commentary -- 8.7 Application Notes -- 8.8 Summary Points -- 8.9 Conclusions -- Acknowledgments -- References -- 9 Biocompatible Genetic Modification of Multipotent Mesenchymal Stromal Cells -- 9.1 Introduction -- 9.2 Materials -- 9.2.1 Reagents -- 9.2.2 Facilities/Equipment -- 9.3 Methods -- 9.3.1 Preparation of Pullulan-Spermine -- 9.3.2 Preparation of Plasmid DNA -- 9.3.3 Isolation and Culture of Primary Rat Bone Marrow MSCs -- 9.3.4 Preparation of MSCs for Transfection in 24-Well Plates -- 9.3.5 Pullulan-Spermine/DNA Anioplex Preparation and Anioplex Transfection -- 9.3.6 WST-8 Assay -- 9.3.7 Luciferase Assay -- 9.3.8 Anioplex-Mediated Ex Vivo Transfection of MSCs Prior to Transplantation -- 9.3.9 Lumbar Puncture Injection of MSCs -- 9.3.10 Luciferase Assay -- 9.4 Data Acquisition, Anticipated Results, and Interpretation -- 9.5 Discussion and Commentary -- 9.6 Application Notes -- 9.7 Summary Points -- Acknowledgments -- References -- 10 Adipose Tissue as a Source of Mesenchymal Stem Cells for Hepatocyte Transplantation -- 10.1 Introduction -- 10.2 Materials -- 10.3 Methods -- 10.3.1 Isolation and Culture of AT-MSCs -- 10.3.2 Flow Cytometry -- 10.3.3 In Vitro Culture and Expansion of BM-MSC, BM-HSC, and HepG2 Cell Line -- 10.3.4 RT-PCR -- 10.3.5 Immunofluorescence -- 10.3.6 Western Blot Analysis -- 10.3.7 LDL Uptake -- 10.3.8 Periodic Acid-Schiff Staining -- 10.3.9 Ammonia Clearance -- 10.3.10 Albumin Production -- 10.3.11 CD105+ AT-MSC-Derived Hepatocyte Transplantation into Mice with CCl4-Induced Injury -- 10.4 Anticipated Results -- 10.4.1 Characterization of AT-MSCs -- 10.5 Discussion and Commentary -- 10.5.1 Hepatocyte Differentiation and Hepatic Regeneration
10.5.2 Uncultured AT-MSCs -- 10.6 Clinical Applications of Uncultured AT-MSCs -- 10.6.1 Breast Reconstruction -- 10.6.2 Cardiovascular Disease -- 10.7 Summary Points -- Acknowledgments -- References -- 11 Culture Protocols for Producing Definitive Endoderm and Pancreatic Lineage Cells from Human ES or iPS Cells -- 11.1 Introduction -- 11.2 Experimental Design -- 11.2.1 Materials -- 11.2.2 Protocols -- 11.3 Data Acquisition, Anticipated Results, and Interpretation -- 11.4 Discussion and Commentary -- 11.5 Application Notes -- 11.6 Summary Points -- References -- 12 Human Placenta Cell Isolation -- 12.1 Introduction -- 12.2 Placenta Embryological Development -- 12.3 Placenta Tissue Structure -- 12.4 Placenta Immunological Properties -- 12.5 Human Epithelial Cells from Amnion -- 12.6 Human Mesenchymal Stromal Cells from Amnion -- 12.7 Human Mesenchymal Stromal Cells from Chorion -- 12.8 Materials -- 12.9 Methods -- 12.9.1 Preparation of Solutions for Isolation and Culture -- 12.9.2 Preparation for Cell Isolation -- 12.9.3 Release Amniotic Epithelial Cells -- 12.9.4 Anticipated Results -- 12.9.5 Release Amniotic Mesenchymal Cells -- 12.9.6 Anticipated Results -- 12.10 Placenta-Isolated Cell Characterization -- 12.11 Cell Banking -- 12.12 Discussion and Commentary -- 12.13 Application Notes -- 12.14 Summary Points -- Acknowledgments -- References -- 13 Liver Repopulation by Fetal Liver Stem/Progenitor Cells -- 13.1 Introduction -- 13.1.1 Background -- 13.1.2 Cell Transplantation Strategies in Rodent Model Systems -- 13.1.3 Fetal Liver Stem/Progenitor Cells as a Source for Liver Repopulation -- 13.2 Experimental Design -- 13.3 Animals -- 13.4 Materials -- 13.4.1 Cell Isolation -- 13.4.2 Cell Transplantation -- 13.4.3 Detection of Transplanted Cells -- 13.5 Methods -- 13.5.1 Cell Isolation -- 13.5.2 Cell Transplantation
13.5.3 Detection of Transplanted Cells
This practical book is part of the new Artech House Methods in Bioengineering series - volumes designed to offer detailed guidance on authoritative methods for addressing specific bioengineering challenges. Written and edited by recognized experts in the field, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome problems that may be encountered. This unique volume covers in detail the basic techniques and protocols of regenerative medicine, focusing on methods for cell transplantation. You explore a wide range of critical factors, such as grown tissues, cells, laboratory-made compounds, and engineered organs. The books helps you understand the many intricate steps involved in tissue engineering, cell isolation, progenitors and stem cell differentiation, and in vivo models for assessing new strategies
Description based on publisher supplied metadata and other sources
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2020. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
鏈接 Print version: Soto-Gutierrez, Alejandro Methods in Bioengineering : Cell Transplantation Norwood : Artech House,c2011 9781608070152
主題 Mathematics -- Authorship.;Mathematics -- Study and teaching (Elementary);Mathematics -- Study and teaching (Primary)
Electronic books
Alt Author Navarro-Alvarez, Nalu
Fox, Ira J
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