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書名 Leptospira spp : Methods and Protocols / edited by Nobuo Koizumi, Mathieu Picardeau
出版項 New York, NY : Springer US : Imprint: Humana, 2020
©2020
國際標準書號 9781071604595
1071604597
國際標準號碼 10.1007/978-1-0716-0459-5
book jacket
版本 1st ed. 2020
說明 1 online resource (X, 291 p. 66 illus., 56 illus. in color.) : online resource
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
text file PDF rda
系列 Methods in Molecular Biology, 1064-3745 ; 2134
Methods in Molecular Biology ; 2134
附註 This volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms.
鏈接 Printed edition 9781071604588
Printed edition 9781071604601
Printed edition 9781071604618
主題 Microbiology
Infectious diseases
Microbiology. fast (OCoLC)fst01019576
Electronic books
Alt Author Koizumi, Nobuo., editor
Picardeau, Mathieu., editor
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