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245 10 Human Monoclonal Antibodies|h[electronic resource] :
       |bMethods and Protocols /|cedited by Michael Steinitz 
250    2nd ed. 2019 
264  1 New York, NY :|bSpringer New York :|bImprint: Humana Press,
       |c2019 
300    XIV, 483 p. 79 illus., 59 illus. in color.|bonline 
       resource 
336    text|btxt|2rdacontent 
337    computer|bc|2rdamedia 
338    online resource|bcr|2rdacarrier 
347    text file|bPDF|2rda 
490 1  Methods in Molecular Biology,|x1064-3745 ;|v1904 
505 0  Human Monocolonal Antibodies: The Benefits of Humanization
       -- Cancer Immunotherapy: The Dawn of Antibody Cocktails --
       IgM Natural Autoantibodies in Physiology and the Treatment
       of Disease -- Monoclonal Antibodies for the Treatment of 
       Melanoma: Present and Future Strategies -- An Efficient 
       Method to Generate Monoclonal Antibodies from Human B 
       Cells -- Isolation of Antigen-Specific, antibody-Secreting
       Cells using a Chip-Based Immunospot Array -- Purification 
       of Human Monoclonal antibodies and Their Fragments -- One-
       Tube Multicolor Flow Cytometry Assay (OTMA) for 
       Comprehensive Immunophenotyping of Peripheral Blood -- 
       Humanization and Simultaneous Optimization of Monoclonal 
       Antibody -- Antibody Fragments Humanization: Beginning 
       with the End in Mind -- Antigen-Specific Human Monoclonal 
       Antibodies from Transgenic Mice -- Refining the Quality of
       Monoclonal Antibodies: Grafting Unique Peptide-Binding 
       Site in the Fab Framework -- Basic Procedures for 
       Detection and Cytotoxicity of Chimeric Antigen Receptors -
       - Rapid Chimerization of Antibodies -- Phage Display 
       Technology for Human Monoclonal Antibodies -- Recombinant 
       Antibody Selections by Combining Phage and Yeast Display -
       - Epitope Mapping via Phage Display from Single Gene 
       Libraries -- A High-Throughput Magnetic Nanoparticle Based
       Semi-Automated Antibody Phage Display Biopanning -- A 
       Binding Potency Assay for Pritumumab and Ecto-Domain 
       Vimentin -- A Method to Detect the Binding of Hyper-
       Glycosylated Fragment Crystallizable (Fc) Region of Human 
       IgG1 to Glycan Receptors -- A Cell-Based Reporter Assay 
       Measuring the Activation of Fc Gamma Receptors Induced by 
       Therapeutic Monoclonal Antibodies -- "BIClonals": 
       Production of Bispecific Antibodies in IgG Format in 
       Transiently Transfected Mammalian Cells -- Production of 
       Stabilized Antibody Fragments in the E. coli Bacterial 
       Cytoplasm and in Transiently-Transfected Mammalian Cells 
520    This second edition volume expands on the previous edition
       with descriptions of recent developments in the field. The
       chapters in this book cover topics such as monoclonal 
       antibodies for the treatment of melanoma; production and 
       purification of human monoclonal antibodies; humanization 
       and optimization of monoclonal antibodies; rapid 
       chimerization of monoclonal antibodies; epitope mapping 
       via phage display from single gene libraries; recombinant 
       antibodies made by combining phage and yeast display 
       selections; production of stabilized antibody fragments in
       the E. coli bacterial cytoplasm and transfected mammalian 
       cells; and analysis of CAR T cells. Written in the highly 
       successful Methods in Molecular Biology series format, 
       chapters include introductions to their respective topics,
       lists of the necessary materials and reagents, step-by-
       step, readily reproducible laboratory protocols, and tips 
       on troubleshooting and avoiding known pitfalls. Unique and
       thorough, Human Monoclonal Antibodies: Methods and 
       Protocols, Second Edition is a valuable tool for novice 
       and expert researchers interested in learning more about 
       this evolving field 
650  0 Monoclonal antibodies 
650 14 Antibodies.|0http://scigraph.springernature.com/things/
       product-market-codes/B14010 
700 1  Steinitz, Michael.|eeditor.|4edt|4http://id.loc.gov/
       vocabulary/relators/edt 
710 2  SpringerLink (Online service) 
773 0  |tSpringer eBooks 
776 08 |iPrinted edition:|z9781493989577 
776 08 |iPrinted edition:|z9781493989591 
830  0 Methods in Molecular Biology ;|v1904 
856 40 |uhttps://doi.org/10.1007/978-1-4939-8958-4
       |zeBook(Springerlink)(Life Science Group only)