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作者 Persson, Staffan Hans
書名 Calreticulins: Calcium-binding proteins with functional diversity and evolutionary duality
國際標準書號 0496466119
book jacket
說明 228 p
附註 Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3036
Director: Wendy F. Boss
Thesis (Ph.D.)--North Carolina State University, 2003
Calreticulin is a multifunctional protein mainly localized in the endoplasmic reticulum in eukaryotic organisms. The protein comprises three distinct regions: the N-terminal N domain, the C-terminal C domain, and the central P domain, reflecting the functional diversity of calreticulin. In animal cells the protein can bind approximately 25 mol Ca2+ per mol protein. Although a similar Ca2+ binding capacity is evident for plant calreticulins, the physiological relevance of the protein in endoplasmic reticulum Ca2+ regulation has been elusive. Nicotiana tabacum suspension cells were therefore transformed with a maize crt cDNA, under the control of a heat-shock promoter. Induction of the calreticulin transgene enhanced the endoplasmic reticulum Ca2+ content in vitro. Furthermore, heat shock-induced Arabidopsis plants, transformed with the same construct, grew better on medium containing low levels of Ca2+ compared with control plants
The multifunctional properties assigned to calreticulins have triggered a search for additional isoforms and for two or more copies of calreticulin genes in mammals. By performing BLASTP searches we found a calreticulin isoform (Crt2) in human, which differed significantly from the previously established isoform. In addition, a homolog to the Crt2 protein was obtained from mouse, and suggested an orthologous Crt2 isoform present in several mammalian species. Similar to the calnexin homolog calmegin, the crt2 gene was exclusively expressed in testis of the tissues investigated
Arabidopsis contains three calreticulin isoforms. Phylogenetic analyses and expression profiling revealed that both monocotyledons and eudicotyledons contain two distinct calreticulin isoform groups: a Crt1/Crt2 and a Crt3 group. Whereas the crt1/crt2 genes were active in all tissue types investigated, peaking in flowers, the crt3 gene was mainly expressed in root and leaf tissues. Furthermore, members from the different isoform groups were induced differently in response to tunicamycin, an inhibitor of N-linked glycosylation. To provide a common research basis for plant calreticulins, a new nomenclature for the proteins was suggested. The discovery of two orthologous calreticulin isoform groups in both animals and plants support an evolutionary duality, and suggest functional diversity for calreticulins
School code: 0155
DDC
Host Item Dissertation Abstracts International 64-07B
主題 Biology, Botany
Biology, Plant Physiology
0309
0817
Alt Author North Carolina State University
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