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作者 Sharma, Anjana Rajen
書名 Characterization of root stimulating bacterium and its root stimulating abilities
國際標準書號 0496552295
book jacket
說明 114 p
附註 Source: Dissertation Abstracts International, Volume: 64-10, Section: B, page: 4713
Adviser: Otto J. Schwarz
Thesis (Ph.D.)--The University of Tennessee, 2003
In this study, the characterization of a novel root stimulating bacterium (RSB) is reported and the root stimulating ability of RSB is compared to Sphingomonas and other related bacteria. A morphological examination of RSB was conducted. Nutritional and biochemical characterization was performed in order to obtain a profile of RSB. Pigment analysis indicated the presence of nostoxanthin, a pigment commonly found in Sphingomonas bacteria. Lipid analysis of RSB revealed the presence of sphingolipids in addition to other fatty acids suggesting similarity with other Sphingomonas bacteria. Pulse-field gel electrophoresis of RSB and Sphingomonas echinoides showed a high level of difference in the DNA of these two organisms suggesting difference at least at the strain level
The root stimulating ability of RSB, Sphingomonas and other related bacteria were evaluated using mung bean bioassays. Results of these bioassays suggest that the spent medium used for culturing RSB and Sphingopyxis macrogoltabida contain acidic and neutral compounds that contribute to their promotion of adventitious root formation whereas the spent medium used to culture Novosphingobium capsulata, Sphingomonas echinoides and Sphingomonas paucimobilis contains root stimulating components of acidic nature. Thin layer chromatography of acidic fractions obtained from RSB, Sphingomonas echinoides, Sphingomonas paucimobilis, Sphingopyxis macrogoltabida and Novosphingobium capsulata spent growth medium showed Rf values close to those for the standards indolelactic acid (ILA) and indoleacetic acid (IAA)
Based on all these tests, RSB most closely resembles bacteria of the Sphingomonas group and the rooting ability observed for RSB is characteristic of some of the Sphingomonas and related bacteria
School code: 0226
DDC
Host Item Dissertation Abstracts International 64-10B
主題 Biology, Botany
Biology, Plant Physiology
Biology, Microbiology
0309
0817
0410
Alt Author The University of Tennessee
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