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書名 Functional organization of the nucleus : a laboratory guide / edited by Barbara A. Hamkalo and Sarah C. R. Elgin
出版項 San Diego : Academic Press, c1991
國際標準書號 0125641354
0123219205 (pbk.)
book jacket
館藏地 索書號 處理狀態 OPAC 訊息 條碼
 生命科學圖書館  QH585 M592 1991  v.35    在架上    30120100044957
說明 xviii, 670 p., 2 p. of plates : ill. (some col.) ; 24 cm
系列 Methods in cell biology ; 35
Methods in cell biology ; 35
附註 Includes bibliographical references and index
Part 1: DNA sequence localization in metaphase and interphase cells by fluorescence in situ hybridization -- Localization of mRNAs by in situ hybridization -- Fluorescent detection of nuclear RNA and DNA: implications for genome organization -- Visualization of DNA sequences in meiotic chromosomes -- Nucleic acid sequence localization by electron microscopic in situ hybridization
Part 2: The use of autoantibodies in the study of nuclear and chromosomal organization -- Meiotic chromosome preparation and protein labeling -- Distribution of chromosomal proteins in polytene chromosomes of drosophila -- The use of monoclonal antibody libraries -- Optical sectioning and three-dimensional reconstruction of diploid and polytene nuclei
Part 3: Yeast minichromosomes -- Nucleosomes of transcriptionally active chromatin: isolation of template-active nucleosomes by affinity chromatography -- The nucleoprotein hybridization method for isolating active and inactive genes as chromatin -- Protein-DNA cross-linking as a means to determine the distribution of proteins on DNA in vivo -- Protein-DNA interactions in vivo - examining genes in saccharomyces cerevisiae and drosophila melanogaster by chromatin footprinting
Part 4: Control of class II gene transcription during in vitro nucleosome assembly -- Systems for the study of nuclear assembly, DNA replication, and nuclear breakdown in xenopus laevis egg extracts -- In vitro nuclear protein import using permeabilized mammalian cells
Part 5: Mutations that affect chromosomal proteins in yeast -- Mutations affecting cell division in drosophila -- Position-effect variegation - an assay for nonhistone chromosomal proteins and chromatin assembly and modifying factors
主題 Cell Nuclei
Cell Nucleus
DNA
Mutation
Alt Author Elgin, Sarah C. R
Hamkalo, Barbara A
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