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書名 Amyloid proteins : methods and protocols / edited by Einar M. Sigurdsson
出版項 Totowa, N.J. : Humana Press, c2005
國際標準書號 1588293378 (hardcover : alk. paper)
1592598749 (pbk. : alk. paper)
book jacket
館藏地 索書號 處理狀態 OPAC 訊息 條碼
 生命科學圖書館  QU25 A531 2005    在架上    30150100267662
說明 xiv, 392 p. : ill. ; 24 cm
系列 Methods in molecular biology ; 299
Methods in molecular biology ; 299
附註 PART I. IN VITRO ASSAYS -- 1 Preparation of Aggregate-Free, Low Molecular Weight Amyloid-β for Assembly and Toxicity Assays -- 2 Determination of Peptide Oligomerization State Using Rapid Photochemical Crosslinking -- 3 In Vitro Preparation of Prefibrillar Intermediates of Amyloid-β and α-Synuclein -- 4 Purification of Recombinant Tau Protein and Preparation of Alzheimer Paired Helical Filaments In Vitro -- 5 Cyclic Amplification of Protein Misfolding and Aggregation -- 6 X-Ray Diffraction Studies of Amyloid Structure -- 7 Molecular Electron Microscopy Approaches to Elucidating the Mechanisms of Protein Fibrillogenesis -- 8 Time-Lapse Atomic Force Microscopy in the Characterization -- 9 Fourier Transform Infrared- and Circular Dichroism Spectroscopies for Amyloid Studies -- 10 Quasielastic Light Scattering for Protein Assembly Studies -- 11 Intrinsic Fluorescent Detection of Tau Conformation and Aggregation -- 12 Quantitative Measurement of Fibrillogenesis by Mass Spectrometry -- PART II. CELL CULTURE ASSAYS -- 13 Isolation and Culturing of Human Vascular Smooth Muscle Cells -- 14 Murine Cerebrovascular Cells as a Cell Culture Model for Cerebral Amyloid Angiopathy: Isolation of Smooth Muscle and Endothelial Cells From Mouse Brain -- 15 Purification of Human Wild Type or Variant Cystatin C From Conditioned Media of Transfected Cells -- 16 Prion Propagation in Cell Culture -- PART III. IN VIVO-RELATED ASSAYS -- 17 Preparation and Propagation of Amyloid-Enhancing Factor -- 18 Purification of Protein AA Subspecies From Amyloid-Rich Human Tissues -- 19 Purification of Transthyretin and Transthyretin Fragments From Amyloid-Rich Human Tissues -- 20 Extraction and Chemical Characterization of Tissue-Deposited Proteins From Minute Diagnostic Biopsy Specimens -- 21 Tissue Extraction Prior to Protein Analysis and A_ Quantitation -- 22 ELISA Method for Measurement of A_ Levels -- 23 Histological Staining of Amyloid-_ in Mouse Brains -- 24 The Mouse Model for Scrapie: Inoculation, Clinical Scoring, and Histopathological Techniques -- 26 In Vivo Imaging of Amyloid-_ Deposits in Mouse Brain With Multiphoton Microscopy -- 27 Magnetic Resonance Imaging of Amyloid Plaques in Transgenic Mice
Includes bibliographical references and index
In vitro assays -- Cell culture assays -- In vivo-related assays
主題 Amyloid -- Laboratory manuals
Amyloid beta-protein -- Laboratory manuals
Alt Author Sigurdsson, Einar M
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