Record:   Prev Next
書名 Basic methods in protein purification and analysis : a laboratory manual / edited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis
出版項 Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2009
國際標準書號 9780879698683 (hardcover : alk. paper)
0879698683 (hardcover : alk. paper)
9780879698676 (pbk. : alk. paper)
0879698675 (pbk. : alk. paper)
book jacket
館藏地 索書號 處理狀態 OPAC 訊息 條碼
 生命科學圖書館  QU25 B3115 2008    在架上  -  30150100306221
 生命科學圖書館  QU25 B3115 2008 c.2  在架上  -  30150100306247
說明 vii, 436 p. : ill. ; 28 cm
附註 Includes bibliographical references and index
Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized pH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools : analysis for creating focused interaction networks
Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized PH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest
主題 Proteins -- Purification -- Laboratory manuals
Proteins -- Analysis -- Laboratory manuals
Proteins -- isolation & purification -- Laboratory Manuals
Chromatography -- methods -- Laboratory Manuals
Electrophoresis -- methods -- Laboratory Manuals
Immunoprecipitation -- methods -- Laboratory Manuals
Proteins -- analysis -- Laboratory Manuals
Alt Author Simpson, Richard J
Adams, Peter D. (Peter David)
Golemis, Erica
Record:   Prev Next